Antibiotic Susceptibility of Escherichia coli Strains Isolated in Community Acquired Urinary Tract Infections (original) (raw)
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2019
In Constanta during the summer hospital admission of children especially with diarrheal diseases is increased because of the influx of tourists. We determine retrospectively the antibiotic susceptibility of E. coli strains isolated from a pediatric population hospitalized during 2012 at Constanta Clinical Infectious Diseases Hospital. Microbiology samples were inoculated on selective media (Blood Agar and Drigalscki Lactose Agar) and incubated at 37 o C, for 16 h, and subsequently on average multitest media (MIU, TSI, and Simmons). For the final identification we used API 20E Biomerieux tests. During 2012 we isolated 112 strains of E. coli-85 strains from stool, 16 from urine, 10 from gastric fluid, 8 from throat, and 3 from other secretions. All E. coli strains isolated from our patients were E. coli enteropathogens. Male: Female ratio was 1:1.33. Median age was 5.5 years (range from 3 months-17.4 years). The antibiotic sensitivity of E. coli revealed a low sensitivity to ampicillin (19.6%), tetracycline (29.5%), and amoxicillin (37.5%). The highest sensitivity was to Carbapenems (93%). Among diseases caused by E. coli in children hospitalized at Constanta Clinical Infectious Diseases Hospital during 2012 intestinal tract infections were the most frequent, followed by urinary tract infections. Antibiotic susceptibility of E. coli was low to ampicillin, and tetracycline, while the majority of E. coli strains were sensitive to carbapenems.
African Journal of Microbiology Research, 2014
In Constanta during the summer hospital admission of children especially with diarrheal diseases is increased because of the influx of tourists. We determine retrospectively the antibiotic susceptibility of E. coli strains isolated from a pediatric population hospitalized during 2012 at Constanta Clinical Infectious Diseases Hospital. Microbiology samples were inoculated on selective media (Blood Agar and Drigalscki Lactose Agar) and incubated at 37 o C, for 16 h, and subsequently on average multitest media (MIU, TSI, and Simmons). For the final identification we used API 20E Biomerieux tests. During 2012 we isolated 112 strains of E. coli-85 strains from stool, 16 from urine, 10 from gastric fluid, 8 from throat, and 3 from other secretions. All E. coli strains isolated from our patients were E. coli enteropathogens. Male: Female ratio was 1:1.33. Median age was 5.5 years (range from 3 months-17.4 years). The antibiotic sensitivity of E. coli revealed a low sensitivity to ampicillin (19.6%), tetracycline (29.5%), and amoxicillin (37.5%). The highest sensitivity was to Carbapenems (93%). Among diseases caused by E. coli in children hospitalized at Constanta Clinical Infectious Diseases Hospital during 2012 intestinal tract infections were the most frequent, followed by urinary tract infections. Antibiotic susceptibility of E. coli was low to ampicillin, and tetracycline, while the majority of E. coli strains were sensitive to carbapenems.
Escherichia coli is an emerging pathogen of the greatest concern as it is the leading cause of various severe infections of stomach, urinary tract, ear, wound etc. in humans. Increasing rates of antimicrobial resistance among E. coli is another furthermost fret worldwide. This problem is more traumatic when water bodies are getting contaminated with faecal pollution and inappropriate use of antibiotics that led to emergence of multi-drug resistant strains of this normal microbiota of human intestine. The current study dealt with the isolation of around twenty Escherichia coli strains from water samples collected from different prominent hilly locations of Uttarakhand region of India. These bacteria were evaluated for their resistance and susceptibility patterns against seven commonly prescribed clinically-significant antibiotics viz. Azithromycin, Cefixime, Ciprofloxacin, Doxycycline, Gentamycin, Streptomycin and Tetracycline. E. coli strains were shown to have variable susceptibilities during the study. Doxycycline was found to be most inert antibiotic as it was not effective against any of E. coli isolates. Around 85% isolates were shown to be sensitive against Cefixime making this antibiotic more promising. More evident finding of the study was that none of antibiotic used in the study was 100% effective. Cefixime was found to be the most effective antimicrobials against E. coli isolates followed by Streptomycin, Tetracycline, Gentamycin, Azithromycin and Ciprofloxacin. Multiple antimicrobial resistances (MAR) index for all the isolates were calculated on the basis of resistance patterns. It has been observed that only 10% strains showed the MAR Index less than 0.1 while rest of 90% isolates were having MAR index between 0.2 to 0.9 showing the multi-drug resistance.
African Journal of Health Sciences, 2016
Background: Diarrheagenic E. coli (DEC) are associated with outbreaks of severe diarrhea and multiple drug resistance. We characterize DEC among diarrhoeal patients attending Thika Hospital and determine their antimicrobial susceptibility patterns. Methods: A cross-sectional study was conducted among patients of all ages seeking diarrhea treatment. Stool samples were collected, inoculated on bacterial differential media for growth of enteric pathogens, characterized and antimicrobial susceptibility of DEC isolates determined. Results: A total of 402 stool samples were cultured. E. coli was isolated from 269, of which 72 (27%) were DEC; 60 (83.3%) enteroaggregative E. coli (EAEC), 6 (8.3%) enteropathogenic E. coli (EPEC) and 6 (8.3%) enterotoxigenic E. coli (ETEC). Of the 72, 58% were female, median age was 8 (IQR: 2-28) years, 75% did not boil water and 100% did not treat water. Twenty five (35%) patients with DEC were under-five years. Drinking unboiled water (OR: 2.51, 95% CI: 1.36-4.61) was associated with having DEC. All DEC isolates were sensitive to cefoxitin, meropenem, amikacin, gentamicin and ciprofloxacin. They were resistance to ampicillin (92%), trimethoprim-sulfamethoxazole (92%) and amoxicillin-clavulanic acid (85%). Conclusion: The predominant DEC strain was EAEC. High resistant to ampicillin, trimethoprim-sulfamethoxazole and amoxicillin-clavulanic acid were observed. All isolates were sensitive to ciprofloxacin and gentamicin.
Journal of Basic Research in Medical Sciences, 2017
Introduction: Escherichia coli (E.coli) as a main cause of both nosocomial and communityacquired infections in humans have a relative potential to develop resistance. Nowadays, most infections caused by ESBL-producing E.coli (ESBLEC) had mostly been described as nosocomial acquired or nursing home related. In this study, we employed E-test assay to detect antibiotic resistance of E.coli strains and determine MIC of antibiotics. Materials and methods: Thirty E.coli strains gathered from Imam Khomeini hospital of Ilam, and cultured on TSB and bacterial suspension prepared by 0.5 µF concentration for Etest. Mueller Hinton agar and E-test strips of Amikacin,
IN VITRO ANTIMICROBIAL SENSITIVITY OF ESCHERICHIA COLI ISOLATED FROM CLINICAL SOURCES
A study of antimicrobial sensitivity of Escherichia coli (E. coli) isolated from clinical sources of different diagnostic centers of Dhaka, Bangladesh was carried out to facilitate the choice of drug in the management of E. coli induced symptoms. Very low sensitivity of E. coli towards ampicillin (4%), aztreonam (4%), cloxacillin (5%), nalidixic acid (5%), ciprofloxacin (7.5%), ceftriaxone (12.5%), doxycycline (12.5%), ceftazidime (16.25%), co-trimoxazole (20%), chloramphenicol (22.51%), tetracycline (25%), and netilmicin (35%) was observed. Higher sensitivity pattern was observed for gentamicin (56%) and only imipenem (95%) showed sensitivity pattern possibly susceptible enough to consider for the management of E. coli induced cases in the area under study. The low sensitivity to different antimicrobial could be attributed to their prevailing usage and abuse in the area under study.
Antimicrobial susceptibility of Escherichia coli isolated from humans and animals
Veterinarija Ir Zootechnika, 2009
The prospective study was conducted in IBN SINA Diagnostics and Imaging center, Dhaka, Bangladesh during May 2007 to May 2008. To ensure appropriate treatment, knowledge of the organisms that cause specific infection and their antibiotic susceptibility is mandatory. In the present study, 40 isolates of E.coli were analyzed in term of antibiotic susceptibility. Result of antimicrobial susceptibility test reveals that all of the isolates were sensitive to Cotrimoxazole (30.0%), Ciprofloxacin (20.0%), Nalidixic acid (10.0%), Ceftriaxone (30.0%), Chloramphenicol (70.0%), Cefuroxime (10.0%), Imepenem (100.0%), Cefixime (70.0%). Multi-drug resistance pattern was observed in 90% of the isolates. 30% isolates were resistant to 8 antibiotics, 40% isolates were resistant to 6 antibiotics, 10% isolates were found to resist 4 antibiotics and 10% isolates were found resistant to 3 antibiotics. Our study concludes that there is a trend towards an increase in resistance to fluoroquinolones in the common gram-negative bacteria. Imipenem is the most active agent against gramnegative bacteria (Escherichia coli).The results of the susceptibility of Escherichia coli causing bacteremia provide valuable information for implementing the appropriate chemotherapy for bacteremia.
Antimicrobial Susceptibility Patterns of Escherichia Coli From Various Clinical Sources
Journal of Chitwan Medical College, 2013
Escherichia coli is the major organism causing the urinary tract infection, wound infection and respiratory tract infection. A total of 2376 samples of urine, wound swab and sputum were analyzed for identification of bacterial isolates and their antimicrobial susceptibility pattern. Escherichia coli (E. coli) was isolated from 357 [15%] samples. Majority of the E.coli, 307 [85.9%], were obtained from the urine samples, followed by wound swab [8.4%] and sputum [5.6%]. High degree of resistance was observed for nalidixic acid [92.8%] followed by ceftriaxone [65.7%] and cotrimoxazole [64.6%]. The isolates were highly sensitive to imipenem (100%) followed by nitrofurantoin [90.3%] and amikacin [82.1%].
In Vitro Antimicrobial Sensitivity of Escherichia coli Isolated from Clinical Sources
Journal of Bangladesh Academy of Sciences, 2010
A study of antimicrobial sensitivity of Escherichia coli (E. coli) isolated from clinical sources of different diagnostic centers of Dhaka, Bangladesh was carried out to facilitate the choice of drug in the management of E. coli induced symptoms. Very low sensitivity of E. coli towards ampicillin (4%), aztreonam (4%), cloxacillin (5%), nalidixic acid (5%), ciprofloxacin (7.5%), ceftriaxone (12.5%), doxycycline (12.5%), ceftazidime (16.25%), co-trimoxazole (20%), chloramphenicol (22.51%), tetracycline (25%), and netilmicin (35%) was observed. Higher sensitivity pattern was observed for gentamicin (56%) and only imipenem (95%) showed sensitivity pattern possibly susceptible enough to consider for the management of E. coli induced cases in the area under study. The low sensitivity to different antimicrobial could be attributed to their prevailing usage and abuse in the area under study.