Influence of Lyophilized Rumen Fluid Challenge on Egyptian Buffalo Calves affected with Simple Indigestion (original) (raw)

Fresh rumen fluid was collected from slaughtered house in Ismailia governorate and lyophilized; then administered for Egyptian buffalo calves suffered from simple indigestion. Ten buffalo calves were divided into 2 groups (Five calves for each group); the 1 st group suffered from simple indigestion and were challenged with lyophilized rumen fluid (LRF) for 7 consecutive days, and a 2 nd control group. Ruminal fluid samples were collected at zero time before challenge; and at 1, 2, and 3 weeks post challenge. The study revealed that buffalo calves suffered from simple indigestion showed a highly significant reduction in ruminal pH, protozoal motility, total protozoal; total bacterial ; lactobacillus and ruminococcus counts in addition to ammonia, SCFA's and glucose level in ruminal fluid. Also, the study revealed a significant increase in the total bacterial; total protozoal counts, and count of lactobacillus and ruminococcus. Furthermore, there were a significant increase of ruminal lactate, ammonia and glucose level after treatment of indigestion with LRF compared with its level at zero time before challenge. Environmental temperature and relative humidity recording in order revealed strong positive; strong positive, strong negative; strong negative, strong positive; very strong positive, weak positive; strong negative, weak negative; positive, weak negative; weak positive, very strong positive; weak positive, weak negative; strong negative, and weak negative; weak positive correlations to ruminal pH; protozoal motility; log TPC; log TBC; log Lactobacillus count; log Ruminococcus count; levels of glucose; lactate; ammonia, respectively.