Dynamics of galanthamine and lycorine contents in long-term in vitro cultures of Leucojum aestivum L. (Amaryllidaceae) (original) (raw)

In vitro clones of Leucojum aestivum cultured 4 years under equal conditions on agar MS medium supplemented with BAP and NAA kept their inherent alkaloid profiles. However, the contents of the main alkaloids Gal and Lyc varied in the course of time.The dynamics were studied in 12 clones for at least 24 months (HPLC analysis every 3 months). Clones differed in the way of the changes of their alkaloid content. Different correlations were proved, using R, between the fluctuations of Gal, Lyc and cultures' dry matter of some clones and between clones.

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