Enzymes: Biochemistry, Biotechnology, Clinical Chemistry, second edition, Horwood, Chichester, 2007, reprinted by Woodhead Publishing, 2011. (original) (raw)

Abstract

Chapters: 1) An introduction to enzymes; 2) The structure of proteins; 3) The biosynthesis and properties of proteins; 4) Specificity of enzyme action; 5) Monomeric and oligomeric enzymes; 6) An introduction to bioenergetics, catalysis and kinetics; 7) Kinetics of single-substrate enzyme-catalysed reactions; 8) Enzyme inhibition; 9) Kinetics of multi-substrate enzyme-catalysed reactions; 10) The investigation of active site structure; 11) The chemical nature of enzyme catalysis; 12) The binding of ligands to proteins; 13) Sigmoidal kinetics and allosteric enzymes; 14) The significance of sigmoidal behaviour; 15) Investigation of enzymes in biological preparations; 16) Extraction and purification of enzymes: 17) Enzymes as analytical reagents; 18) Instrumental techniques available for use in enzymatic analysis; 19) Applications of enzymatic analysis in medicine, forensic science and industry; 20) Biotechnological applications of enzymes; 21) Genomics, proteomics and bioinformatics.

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