Silver-containing calcium phosphate materials of marine origin with antibacterial activity (original) (raw)
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Study of Antimicrobial Activity of Silver-Doped Hydroxyapatite
Asian Journal of Pharmaceutical and Clinical Research, 2019
Objective: Hydroxyapatite (Hap) is an important material used for implants because of its structural similarity with calcium phosphate of the teeth and bones. Hap is mostly used in the situations where load-bearing implants are needed, such as in dental and orthopedic applications. Dental plaque is caused due to the bacteria which induces secondary caries through microleakage between the restoration and the tooth structure. Methods: An attempt has been made to provide materials with antibacterial activity by incorporating an antibacterial agent into the chemical mixture (Hap). This study delineates the process of synthesizing silver-doped Hap (Ca10Agx(PO4)6(OH)2) by wet chemical precipitation method and by varying the silver concentration (0.3%–0.5%). The synthesized silver-doped Hap was further characterized using Fourier transform infrared spectroscopy and X-ray diffraction (XRD) analysis. The antimicrobial activities were tested against Gram-negative bacteria Escherichia coli. Re...
Development of Antibiotics Impregnated Nanosized Silver Phosphate-Doped Hydroxyapatite Bone Graft
Journal of Nanomaterials, 2013
Nanosized Ag3PO4loaded hydroxyapatite which was prepared by a novel low temperature phosphorization of 3D printed calcium sulfate dihydrate at the nominal silver concentration of 0.001 M and 0.005 M was impregnated by two antibiotics including gentamicin and vancomycin. Phase composition, microstructure, antibiotics loading, silver content, antimicrobial performance, and cytotoxic potential of the prepared samples were characterized. It was found that the fabricated sample consisted of hydroxyapatite as a main phase and spherical-shaped silver phosphate nanoparticles distributing within the cluster of hydroxyapatite crystals. Antibacterial activity of the samples against two bacterial strains (gram negativeP. aeruginosaand gram positiveS. aureus) was carried out. It was found that the combination of antibiotics and nanosized Ag3PO4in hydroxyapatite could enhance the antibacterial performance of the samples by increasing the duration in which the materials exhibited antibacterial pro...
Journal of Materials Science: Materials in Medicine, 2021
Combination of bioactive material such as hydroxyapatite (HAp) with antibacterial agents would have great potential to be used as bone implant materials to avert possible bacterial infection that can lead to implant-associated diseases. The present study aimed to develop an antibacterial silver nanoparticle-decorated hydroxyapatite (HAp/AgNPs) nanocomposite using chemical reduction and thermal calcination approaches. In this work, natural HAp that was extracted from chicken bone wastes is used as support matrix for the deposition of silver nanoparticles (AgNPs) to produce HAp/AgNPs nanocomposite. XRD, FESEM-EDX, HRTEM, and XPS analyses confirmed that spherical AgNPs were successfully synthesized and deposited on the surface of HAp particles, and the amount of AgNPs adhered on the HAp surface increased with increasing AgNO3 concentration used. The synthesized HAp/AgNPs nanocomposites demonstrated strong antibacterial activity against Staphylococcus aureus bacteria, where the antibact...
Antibacterial nanosized silver substituted hydroxyapatite: Synthesis and characterization
The silver (0.5–3 at %) substituted nanosize hydroxyapatites (AgHAs) were synthesized by microwave processing. The X-ray diffraction (XRD) peaks are very broad, indicating that the AgHAs were of nanosize (30 nm). Transmission electron microscopy analysis shows needle-like morphology of AgHA, having length 60–70 nm and width 15–20 nm. The AgHA phase was stable up to 7008C without any secondary phases. The antibacterial effect of AgHA against Escherichia coli and Staphylococcus aureus was observed by spread plate method, even for low concentration of silver ions (0.5%) with 1 Â 10 5 cells/ mL of respective bacterial culture, after a 48 h incubation period. However, some colonies of E. coli were seen with a high dose of 1 Â 10 8 cells/mL after 24 h. The zone of inhibition by disc diffusion test method was found to vary with the amount of silver in the sintered AgHA pellets , for both the bacteria, after 24 h of inoculation. Osteo-blast cell attachment in varying density was noticed on AgHA samples with 0.5, 1.0, and 1.5% silver substitution. However, osteoblast spreading was significantly greater on 0.5% AgHA compared to 1.0 or 1.5% substituted AgHA samples. Thus, the low amount of AgHA has a potential of minimizing the risk of bacterial contamination , without compromising the bioactivity, and is expected to display greater biological efficacy in terms of osseointegration.