Sphingomonas alaskensis sp. nov., a dominant bacterium from a marine oligotrophic environment (original) (raw)
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Sphingomonas daechungensis sp. nov., isolated from sediment of a eutrophic reservoir
International Journal of Systematic and Evolutionary Microbiology, 2014
Strain CH15-11T, isolated from a sediment sample taken from Daechung Reservoir, South Korea, during the late-blooming period of cyanobacteria, was found to be a Gram-stain-negative, non-motile, non-spore-forming, rod-shaped and aerobic bacterium. Strain CH15-11T grew optimally at pH 7 and 28–30 °C. According to a phylogenetic tree based on 16S rRNA gene sequences, strain CH15-11T belonged to the genus Sphingomonas and clustered with Sphingomonas sediminicola Dae 20T, with which it shared the highest 16S rRNA gene sequence similarity (97.6 %). Chemotaxonomic analysis showed that strain CH15-11T had characteristics typical of members of the genus Sphingomonas , such as the presence of sphingoglycolipid, ubiquinone Q-10 and sym-homospermidine. Plus, strain CH15-11T included summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0 as the major fatty acids. The genomic DNA G+C content was 65.6 mol%. Sequence data showed that strain CH15-11T was most closely related to Sphingomonas sed...
Sphingomonas aquatica sp. nov., isolated from tap water
International journal of systematic and evolutionary microbiology, 2016
A gram-reaction-negative, strictly aerobic, rod-shaped bacterium, designated W1-2-1T, was isolated from tap water of South Korea. The strain was characterized by a polyphasic approach to clarify its taxonomic position. Strain W1-2-1T grew at 18-42 °C and at pH 6.0-10.0 on R2A medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Sphingomonas and is most closely related to the Sphingomonas oligophenolica JCM 12082T (97.2 % similarity), Sphingomonas asaccharolytica IFO 15499T (96.8%), Sphingomonas desiccabilis CP1DT (96.8 %), Sphingomonas pruni IFO 15498T (96.8%), Sphingomonas hankookensis ODN7T (96.4 %), and Sphingomonas yabuuchiae DSM 14562T (95.8%). Chemotaxonomic data [major ubiquinone-Q10, major polyamine-homospermidine, major fatty acids- summed feature 8 (C18:1 ω7c/ω9c), C16:0, and C14:0 2OH and presence of sphingoglycolipid] supported the affiliation of the strain to the genus Sphingomonas. The G+C content of genomic DNA...
Sphingomonas agri sp. nov., a bacterium isolated from soil
International Journal of Systematic and Evolutionary Microbiology
A Gram-reaction-negative, aerobic, non-motile, white (translucent) and rod-shaped bacterium (designated HKS-06 T) isolated from soil was characterized by a polyphasic approach to clarify its taxonomic position. Strain HKS-06 T was observed to grow optimally at 30 C and at pH 6.5-7.0 on R2A agar medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HKS-06 T belongs to the genus Sphingomonas and is most closely related to Sphingomonas lutea JS5 T (97.4 % similarity). The G+C content of the genomic DNA was 64.1 mol%. Chemotaxonomic data [major quinone (Q-10), major polar lipids (phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine, unknown polar lipid) and major fatty acids (summed feature 8, comprising C 18 : 1 !7c/!6c and/or C 18 : 1 !6c, C 18 : 0 3-OH and C 16 : 0)] supported the affiliation of strain HKS-06 T to the genus Sphingomonas. Moreover, the physiological and biochemical results and low level of DNA-DNA relatedness [between strain HKS06 T and S. lutea JS5 T (20.24±1.2 %)] allowed the phenotypic and genotypic differentiation of strain HKS-06 T from recognized species of the genus Sphingomonas. The new isolate therefore represents a novel species, for which the name Sphingomonas agri sp. nov. is proposed. The type strain is HKS-06 T (=KACC 18880 T =LMG 29563 T).
Sphingomonas aestuarii sp. nov., isolated from tidal flat sediment
International journal of systematic and evolutionary microbiology, 2009
A novel bacterium (strain K4 T ) belonging to the genus Sphingomonas was isolated from tidal flat sediment in Korea. Its morphology, physiology, biochemical features and 16S rRNA gene sequence were characterized. Colonies of this strain are yellow in colour and the cells are rodshaped, exhibiting negative Gram staining. The strain grows at 0-5 % (w/v) NaCl and 20-35 6C, with optimal growth occurring at 0 % (w/v) NaCl and 30 6C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K4 T is associated with the genus Sphingomonas. Within the phylogenetic tree, this novel strain shares a branching point with Sphingomonas asaccharolytica Y-345 T , with which it shares 97.3 % 16S rRNA gene sequence similarity. The polyamine pattern predominantly contains the Sphingomonas-specific triamine symhomospermidine. Combined analysis of 16S rRNA gene sequences, DNA-DNA relatedness, physiological and biochemical test results identified genotypic and phenotypic differences between strain K4 T and other Sphingomonas species. On the basis of these differentiating features, it is concluded that strain K4 T (5KCTC 22050 T 5DSM 19475 T ) represents a novel species of the genus Sphingomonas, for which the name Sphingomonas aestuarii sp. nov. is proposed.
Applied and Environmental Microbiology, 2001
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Sphingomonads from marine environments
Journal of Industrial Microbiology and Biotechnology, 1999
Sphingomonas species play an important role in the ecology of a range of marine habitats. Isolates and 16S-rRNA clones have been obtained from corals, natural and artificial sources of marine hydrocarbons and eutrophic and oligotrophic waters, and have been isolated as hosts for marine phages. In addition they are found in oceans spanning temperature ranges from polar to temperate waters. While less is known about marine sphingomonads in comparison to their terrestrial counterparts, their importance in microbial ecology is evident. This is illustrated by, for example, the numerical dominance of strain RB2256 in oligotrophic waters. Furthermore, the known marine sphingomonads represent a phylogenetic cross-section of the Sphingomonas genus. This review focuses on our present knowledge of cultured isolates and 16S-rDNA clones from marine environments.
International journal of systematic bacteriology, 1997
Pseudomonus azotocolligans" ATCC 12417T (T = type strain), which was described as a diazotrophic bacterium, was reinvestigated to clarify its taxonomic position. 16s ribosomal DNA sequence comparisons demonstrated that this strain clusters phylogenetically with species of the genus Sphingornonas and represents a new species. The results of investigations of the fatty acid patterns, polar lipid profiles, and quinone system supported this placement. The substrate utilization profile and biochemical characteristics displayed no obvious similarity to the characteristics of any previously described species of the genus Sphingomonas. The new name Sphingomonus trueperi is proposed on the basis of these results and previously published data for the G+C content of the genomic DNA and the polyamine pattern.
Sphingomonas cynarae sp. nov., a proteobacterium that produces an unusual type of sphingan
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2013
Strain SPC-1 T was isolated from the phyllosphere of Cynara cardunculus L. var. sylvestris (Lamk) Fiori (wild cardoon), a Mediterranean native plant considered to be the wild ancestor of the globe artichoke and cultivated cardoon. This Gram-stain-negative, catalase-positive, oxidase-negative, non-spore-forming, rod-shaped and non-motile strain secreted copious amounts of an exopolysaccharide, formed slimy, viscous, orange-pigmented colonies and grew optimally at around pH 6.0-6.5 and 26-30 6C in the presence of 0-0.5 % NaCl. Phylogenetic analysis based on comparisons of 16S rRNA gene sequences demonstrated that SPC-1 T clustered together with species of the genus Sphingomonas sensu stricto. The G+C content of the DNA (66.1 mol%), the presence of Q-10 as the predominant ubiquinone, sym-homospermidine as the predominant polyamine, 2-hydroxymyristic acid (C 14 : 0 2-OH) as the major hydroxylated fatty acid, the absence of 3-hydroxy fatty acids and the presence of sphingoglycolipid supported this taxonomic position. 16S rRNA gene sequence analysis showed that SPC-1 T was most closely related to Sphingomonas hankookensis ODN7 T , Sphingomonas insulae DS-28 T and Sphingomonas panni C52 T (98.19, 97.91 and 97.11 % sequence similarities, respectively). However, DNA-DNA hybridization analysis did not reveal any relatedness at the species level. Further differences were apparent in biochemical traits, and fatty acid, quinone and polyamine profiles leading us to conclude that strain SPC-1 T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas cynarae sp. nov. is proposed; the type strain is SPC-1 T (5JCM 17498 T 5ITEM 13494 T ). A component analysis of the exopolysaccharide suggested that it represents a novel type of sphingan containing glucose, rhamnose, mannose and galactose, while glucuronic acid, which is commonly found in sphingans, was not detected.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2007
Sphingomonas fennica sp. nov. and Sphingomonas haloaromaticamans sp. nov., outliers of the genus Sphingomonas Bacterial isolates obtained from polychlorophenol-contaminated sites in Finland (strain K101 T ) and from a Dutch drinking water well (strain A175 T ) were characterized taxonomically. 16S rRNA gene sequence analysis, determination of DNA G+C content, physiological characterization, estimation of the ubiquinone and polar lipid patterns and fatty acid content revealed that strains K101 T and A175 T were similar to Sphingomonas wittichii RW1 T but also showed pronounced differences. The DNA G+C contents of the two novel strains were 63.6 and 66.1 mol%, respectively. On the basis of these results, two novel species of the genus Sphingomonas are described, for which the names Sphingomonas haloaromaticamans sp. nov. [type strain A175 T (5DSM 13477 T 5CCUG 53463 T )] and Sphingomonas fennica sp. nov. [type strain K101 T (5DSM 13665 T 5CCUG 53462 T )] are proposed.