Characterization of Salmonella enterica serovar Typhimurium isolates associated with septicaemia in swine (original) (raw)

In th1s study we characterized, usmg genotyping and phenotypmg methods, ISolates {n=33) from septicaemia outbreaks in swine herds as well as isolates {n=33) recovered from healthy ammals at slaughter. We determined the antimicrobial agents resistance profiles using 24 different an timicrobial agents by the d1sk d1ffus1on on agar method, the phage type, the plasmid profiles and the PFGE profiles usmg Xbal and Spel as restriction enzymes for each isolates. Resistance to as much as 10 antimicrobial agents was found in both categories of isolates. A greater number of PFGE genotypes was observed in isolates from septicaemia. Various phage types were 1dent1fied in both groups of isolates. Among the DT1 04 phage type, many genetic clusters were 1dent1fied Analysis of plasmid profiles indicated that septicemic strains possess higher molecular we1ght plasmids than asymptomatic 1solates. These results Indicated that strams associated w1th sept1caem1a belong to various genet1c lineages and suggest that VIrulence tra1ts are assoc1ated w1th plasmid profiles of stra1ns. Our results also suggest that the genetic d1vers1ty of Salmonella DT 104 m1ght be higher m North Amenca if we cons1der results of s1milar studies m Europe