Endogenous Hydrogen Peroxide Plays a Positive Role in the Upregulation of Heme Oxygenase and Acclimation to Oxidative Stress in Wheat Seedling Leaves (original) (raw)

2009, Journal of Integrative Plant Biology

1 2 Pretreatment of lower H 2 O 2 doses (0.05, 0.5 and 5 mM) for 24 h was able to 3 dose-dependently attenuate lipid peroxidation in wheat seedling leaves mediated by 4 further oxidative damage elicited by higher dose of H 2 O 2 (150 mM) for 6 h, also 0.5 5 mM H 2 O 2 being the most effective concentrations. Further results illustrated that 0.5 6 mM H 2 O 2 pretreatment triggered the biphasic production of H 2 O 2 during 24-h period. 7 We also noticed that only peak I (0.25 h) rather than peak II ) was approximately 8 consistent with the enhancement of heme oxygenase (HO) activity, HO-1 gene 9 expression. Meanwhile, enhanced SOD activity, Mn-SOD and Cu,Zn-SOD transcripts 10 might be a potential source of peak I of endogenous H 2 O 2 . Further results confirmed 11 that 0.5 mM H 2 O 2 treatment for 0.5 h was able to up-regulate HO gene expression, 12 which was detected by enzyme activity determination, semi-quantitative RT-PCR and 13 western blotting. Meanwhile, the application of DMTU, a trap for endogenous H 2 O 2 , 14 not only blocked the up-regulation of HO, but also reversed the corresponding 15 oxidation attenuation. Together, above results suggested that endogenous H 2 O 2 16 production (peak I) plays a positive role in the induction of HO by enhancing its 17 mRNA level and protein expression, thus leading to the acclimation to oxidative 18 stress. 19 20

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