Toxin-types of Clostridium perfringens strains isolated from sheep, cattle and paddock soils in Nigeria (original) (raw)
Related papers
2018
In the present study, prevalence of Clostridium perfringens in the healthy and diseased sheep of Kashmir valley was investigated along with the detection of the various toxinotypes present. A total of One hundred (100) faecal samples of ovine population comprising of those taken from healthy flock (50) and from diarrheic cases (50) of the organized and unorganized sectors of Kashmir valley were collected for screening of Clostridium perfringens. On complete isolation and identification, a total of 61 samples were found to be positive for the presence of C. perfringens with a prevalence of 42% and 80% from healthy and diarrheic cases respectively. Molecular typing of the isolates by multiplex PCR employing cpa, cpb, etx, and iap genes revealed 37(60.65%) isolates were Type A, 2 (3.27%) were Type B, 1(1.63%) Type C, and 21(34.42%) were Type D respectively, while no isolate of C. perfringens Type E was recovered from any of the samples collected.
International Journal of Enteric Pathogens
Background: Clostridium perfringens is an important anaerobic bacterium found in the intestine of some livestock. It is concerned with the etiology of some diseases including enterotoxaemia. Various diseases are caused by different types of C. perfringens. Nonetheless, there is no published research on molecular typing and distribution of this pathogenic microorganism in Fars province. Objectives: Accordingly, our study focused on the isolation and toxin typing of C. perfringens from sheep, cattle, and goats in different parts of Fars province by the culture and the polymerase chain reaction (PCR) method. Materials and Methods: Approximately 459 fecal samples were collected and cultured on defined media for the isolation of C. perfringens. The confirmed isolates were genotyped by the PCR method using specific primers. Results: C. perfringens was isolated from 30.93% of the total samples. The results of toxin typing showed a total of 76 (54%), 13 (9%), 30 (21%), and 23 (16%) isolates...
Occurrence of Clostridium perfringens β2 toxin in healthy sheep from Kashmir valley
Journal of entomology and zoology studies, 2020
Clostridium perfringensis a rod shaped, Gram-positive, anaerobic spore forming bacterium, able to produce various toxins which are responsible for various lesions and clinical symptoms in livestock. Clostridium perfringens has the ability to produce many toxins viz., alpha, beta, theta, iota, epsilon and kappa. In addition to the above mentioned toxins, C. perfringens can also produce a novel beta2 (β2) toxin. In the present study 415 samples were collected from healthy sheep from Kashmir valley. The isolates were confirmed by 16S rRNA specific PCR that revealed, 265 (63.85%) samples positive for C. perfringens. These 265 isolates were further screened for four toxin genes viz., cpa, cpb, etx and cbp2 using a multiplex PCR. All C. perfringens isolates were positive for α toxin gene, but not forβ, or, therefore, all strains were identified as type A C. perfringens.72.17% of the isolates harboured both α &β2 toxin gene. The high (100%) occurrence of C. perfringens was observed, even ...