Evaluation of diagnostic methods for the detection of Helicobacter pylori in gastric biopsy specimens of dyspeptic patients (original) (raw)
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Comparison of 4 laboratory methods for detection of Helicobacter pylori
Saudi Medical Journal, 2008
H elicobacter pylori (H. pylori) a global human pathogen, is a major cause of gastritis and gastritisrelated diseases and a precursor to the development of gastric carcinoma. 1-3 Multiple diagnostic methods are available for the detection of H. pylori infection, and these are put into 2 categories: invasive and non-invasive. The invasive tests include culture, histology, campylobacter-530
Diagnostic methods for Helicobacter pylori infection: ideals, options, and limitations
European Journal of Clinical Microbiology & Infectious Diseases, 2018
Helicobacter pylori (H. pylori) resides in the stomach, colonizes gastric epithelium, and causes several digestive system diseases. Several diagnostic methods utilizing invasive or non-invasive techniques with varying levels of sensitivity and specificity are developed to detect H. pylori infection. Selection of one or more diagnostic tests will depend on the clinical conditions, the experience of the clinician, cost, sensitivity, and specificity. Invasive methods require endoscopy with biopsies of gastric tissues for the histology, culture, and rapid urease test. Among non-invasive tests, urea breath test and fecal antigen tests are a quick diagnostic procedure with comparable accuracy to biopsy-based techniques and are methods of choice in the test and treatment setting. Other techniques such as serological methods to detect immunoglobulin G antibodies to H. pylori can show high accuracy as other non-invasive and invasive biopsies, but do not differentiate between current or past H. pylori infections. Polymerase chain reaction (PCR) is an emerging option that can be categorized as invasive and non-invasive tests. PCR method is beneficial to detect H. pylori from gastric biopsies without the need for the cultures. There is no other chronic gastrointestinal infection such as H. pylori with a set of comparable diagnostic methodologies. Despite the availability of multiple diagnostic methods, it remains unclear on the choice of any one method as the gold standard for detecting H. pylori infection, especially in epidemiological studies. In this work, we review the principal diagnostic methods used to detect H. pylori infection and their advantages and disadvantages, and applications in clinical practice.
Istanbul Medical Journal, 2018
Helicobacter pylori is a common pathogen that colonizes the gastric epithelium with a high infection prevalence (1). H. pylori infection risk increases due to insufficiency of socioeconomic conditions and inability to create healthy living conditions. Many gastrointestinal illnesses are associated with H. pylori such as gastritis, gastric and duodenal ulcer, and malignancies (2). Gastroduodenal ulcers can cause gastrointestinal bleeding. H. pylori is detected in more than 70% of cases of gastric ulcer and 90% of cases of duodenal ulcer (3). Its eradication leads to a significant reduction in the incidence of recurrent upper gastrointestinal bleeding (4). Many conventional diagnostic methods fail to identify H. pylori especially in patients with upper gastrointestinal bleeding. However, some studies have shown that polymerase chain reaction (PCR)-based methods are more reliable than the other techniques for the diagnosis of cases with upper gastrointestinal bleeding (5). The accurate detection of H. pylori is essential for the management and eradication of bacteria in related cases. The diagnosis is based on both invasive and non-invasive methods. Invasive diagnostic tests include endoscopy followed by histopathological examination of biopsy specimens, fast urease test, and direct identification of the microorganism using culture. Non-invasive methods comprise urea breath test, antibody detection using serology, and stool antigen test (6). The aim of the current study was to compare invasive and non-invasive tests for the detection of H. pylori in patients with gastroduodenal disease. Antibiotic susceptibility test results were also evaluated for H. pylori strains. Methods Patients The present study was planned retrospectively. Data included 87 patients who had been evaluated
Background: Helicobacter pylori are well recognized as a major cause of gastrointestinal illnesses and gastric cancers. Therefore, the current study aimed to assess different methods for detection of H. pylori in the oral cavity (saliva and dental plaque) and in gastric biopsy among patients with gastric affection, as well as, detection of H. pylori antigen in stool, moreover, to evaluate the antibiotic susceptibility testing of the isolated strains. Methods: Specimens were obtained from . Thirty patients were subjected to detailed history and different sampling; gastric biopsy, oral and stool samples. The oral and gastric samples were processed and cultured. Thereafter, microscopic examination and rapid urease tests (RUTs) were conducted. H. pylori antigen detection was carried out in the stool samples, as well as, susceptibility testing to several antibiotics for all isolates identified. Results: The selected patients had a mean age of 36.23 + 6.317 years. They included 17 males (56.7%) and 13 females (43.3%). 90% of the cases were found positive by culture of the gastric biopsies, while, 96.7% were positive in oral cultures. 92.5% of the gastric samples showed positive results by microscopic examination, however, RUTs were positive in 63.3% of the gastric samples and in 73.3% of the oral samples, meanwhile, 66.7% of patients were found positive by testing their stool for H. pylori antigens. The prevalence of resistance among gastric and oral isolates to Amoxycillin, Amoxycillin/Clavulinic acid, Ampicillin/Sulbactam, Clarithromycin , Tetracycillin and Metronidazole were; (3.7 and 17.2 %), (11.1 and 24.1 %), (11.1 and 20.7 %), (11.1 and 24.1 %), (25.9 and 37.9 %) and (96.3 and 100 %) respectively. Conclusion: There is an evidenced association between gastric affection and oral H. pylori recognition that, even exceeds stool detection of H. pylori antigen. Moreover, continuous evaluation of antibiotic susceptibility should be carried out and clinicians should be aware about it to select the appropriate empiric regimen for H. pylori eradication. [Nermin H. Ibrahim , Azza Abdulazim Gomaa, Mohamed Ahmed Abu-Sief, Tamer M. Hifnawy and Mervat Abd El-Baseer Tohamy The use of different laboratory methods in diagnosis of Helicobacter pylori infection; a comparative study Life Sci J 2012; 9(4):249-259]. (ISSN: 1097-8135).
Evaluation of Invasive and Noninvasive Methods for the Diagnosis of Helicobacter Pylori Infection
PubMed, 2016
Objective: The present study was conducted to evaluate invasive and noninvasive diagnostic methods for detection of Helicobacter pylori (H. pylori) in patients admitted with dyspeptic complaints and to compare sensitivities and specificities. Method: Sets of four gastric biopsy specimens were obtained from a total of 126 patients included in the study. The presence of H. pylori was determined by invasive tests including culture, rapid urease test, polymerase chain reaction (PCR) and histopathology. Among noninvasive tests, urea breath test, serological tests and enzyme-linked immunosorbent assay (ELISA) were performed. Results: H. pylori was isolated in 79 (62.7%) gastric biopsy cultures, whereas positivity was concluded for 105 (83.3%) patients by rapid urease test, for 106 (84.1%) by PCR, for 110 (87.3%) by histopathology, for 119 (94.4%) by urea breath test, and for 107 (84.9%) by ELISA. In the present study, the culture findings and histopathological examination findings were accepted as gold standard. According to the gold standard, urea breath test had the highest sensitivity (96.5%) and the lowest specificity (30%), whereas culture and histopathology had the highest specificities (100%). Conclusion: The use of PCR invasively with gastric biopsy samples yielded parallel results with the gold standard. PCR can be recommended for routine use in the diagnosis of H. pylori.
Contemporary Diagnostic Strategies for the Detection of Helicobacter pylori Infection
Gastroenterology & hepatology, 2012
Helicobacter pylori infection is highly prevalent, affecting approximately half of the world's population. While the majority of infected individuals are asymptomatic, H. pylori infection is associated with certain diseases, including peptic ulcers (either duodenal or gastric), gastritis, and 2 malignancies-gastric cancer and gastric mucosa-associated lymphoid tissue lymphoma. Many of the epidemiologic associations between these diseases and H. pylori infection have been further validated by treatment studies, which show that effective eradication therapy correlates with a decreased risk of disease. A variety of testing strategies are used to detect H. pylori infection. Serologic techniques are widely available and inexpensive, but they are no longer preferred as they have low sensitivities and specificities, and they may show a positive result for a long period following effective therapy. The remaining testing methods are divided into 2 categories: invasive tests (which requir...
Efficacy of Different Laboratory Tests to Diagnose Helicobacter pylori Infection
Faridpur Medical College Journal, 2013
Helicobacter pylori is a Gram negative bacteria which causes chronic gastritis, peptic ulcer disease, primary B-cell gastric lymphoma, and adenocarcinoma of the stomach. There are a set of laboratory tests to diagnose H. pylori infection with a variable accuracy, they are divided into non-invasive tests and invasive tests. Non-invasive tests include serology, urea breath test (UBT) and stool antigen test (SAT). Invasive tests include rapid urease test (RUT), histology and culture. This cross sectional study was carried out in the Department of Gastroenterology, Bangabandhu Sheikh Mujib Medical University (BSMMU) and H. pylori laboratory of International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B) from July 2008 to September 2009 to evaluate the efficacy of RUT, SAT and Culture as a diagnostic tool for H. pylori. Dyspeptic patients were collected from outpatient department of BSMMU. Out of 224 dyspeptic patients 149 patients had ulcers or erosions in the stomach or d...
The present study was conducted to evaluate invasive and noninvasive diagnostic methods for detection of Helicobacter pylori (H. pylori) in patients admitted with dyspeptic complaints and to compare sensitivities and specificities. Method: Sets of four gastric biopsy specimens were obtained from a total of 126 patients included in the study. The presence of H. pylori was determined by invasive tests including culture, rapid urease test, polymerase chain reaction (PCR) and histopathology. Among noninvasive tests, urea breath test, serological tests and enzyme-linked immunosorbent assay (ELISA) were performed. Results: H. pylori was isolated in 79 (62.7%) gastric biopsy cultures, whereas positivity was concluded for 105 (83.3%) patients by rapid urease test, for 106 (84.1%) by PCR, for 110 (87.3%) by histopathology, for 119 (94.4%) by urea breath test, and for 107 (84.9%) by ELISA. In the present study, the culture findings and histopathological examination findings were accepted as gold standard. According to the gold standard, urea breath test had the highest sensitivity (96.5%) and the lowest specificity (30%), whereas culture and histopathology had the highest specificities (100%). Conclusion: The use of PCR invasively with gastric biopsy samples yielded parallel results with the gold standard. PCR can be recommended for routine use in the diagnosis of H. pylori.
Background: Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium it is one of the most common human-specific pathogens which exclusively inhabits the gastric mucosa. Infection with H. pylori is always associated with chronic gastric inflammation, gastritis and peptic ulceration which can lead to gastric cancers such as adenocarcinoma. The aim of this study was to compare between three non invasive methods used for diagnosing Helicobacter pylori infection. Methodology: this study was conducted in Khartoum state from February to May 2017. Stool and serum samples were collected from 105 patients with gastric disorders. The presence of H.pylori infection was evaluated by urea breath test, stool antigen detection test and antibody detection test. Results: A total number of 45 patients gave positive result in urea breath test while 60 patients' showed negative result.44 patients gave positive result in stool antigen test while 61 patients showed negative result. A total number of 40 patients gave positive result in antibody detection test while 65 patients' showed negative result. The result showed that the similarity between urea breath test, stool antigen test, urea breath test, antibody detection test and stool antigen test, antibody detection test was 99%, 72,4% and 71,4 % respectively.