Use ofK L a as a criterion for scaling up the inulinase fermentation process (original) (raw)
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Comparative study for obtaining inulinase and invertase by yeasts
Romanian Biotechnological Letters, 2021
The aim of this comparative study was to obtain a model for production of inulinase and invertase by species Saccharomyces, Candida and Hansenula, strains from culture collection of INCDCF-ICCF, using submerged fermentation in a medium containing inulin as source of C. This model explained the data variation and the actual relationships between the parameters and responses. The dry biomass content as well as the production of inulinase and invertase in the bioprocess medium was influenced by inulin concentration and microelement composition. The main parameters for bioprocesses were: inoculum size 2% (v/v), pH 6, temperature 280 C and 220 rpm agitation speed. Following comparative study for production of extracellular inulinase (exo and endo inulinase) and invertase were obtained for Candida arborea the best results, invertase production having significantly higher concentrations than inulinase (35.92 U/mL invertase activity vs. 8.01 U/mL inulinase activity), on M5 medium. These res...
Parameters Optimization for Increased Intracellular Inulinase Activity of a Yeast Strain
Ecological Engineering and Environment Protection
This study reveals the selection of a yeast strain, possessing inulinase activity and finding the optimal conditions of cultivation. Intra- and extracellular activity assay was performed after cultivation on media, containing inulin as a sole source of carbon. Optimization of the cultivation conditions was carried out for establishing the favorable conditions for biosynthesis of inulinase. Modifying the physicochemical and nutritional parameters of a cultivation process lead to major improvement of the enzyme activity. Highest intra- and extracellular inulinase activity was registered when 1.5% inulin was used, 5 % inoculum, temperature 28°C, pH=6.5 and agitation of 200rpm. The selected strain Kluyveromyces sp. C showed higher values for the intracellular inulinase activity, making it suitable for immobilization and further use. Key words: Kluyveromyces sp., inulinase activity, parameters optimization
Optimisation of inulinase production by Kluyveromyces bulgaricus
Web Science, 2002
Present work is based on observation of effects of pH and temperature of fermentation on the production of microbial enzyme inulinase by Kluyveromyces bulgaricus (former Kluyveromyces marxianus). Inulinase hydrolyses inulin, an oligosaccharide which can be isolated from plants such as Jerusalem artichoke, chicory or dahlia, into pure fructose (1). Fructooligosaccharides have great potential in food industry because they can be used as calorie-reduced and noncariogenic sweeteners. Fructose formation from inulin is a single step enzymatic reaction and yields are up to 95 % fructose. On contrary, conventional fructose production from starch needs at least three enzymatic steps, yielding only 45 % fructose (2). Process of inulinase production was optimised by using experimental design method. pH value of the cultivation medium showed to be the most significant variable and it should be maintained at optimum value, 3.6. The effect of temperature was slightly lower and optimal values are between 30 and 33 ºC. At a low pH value of the cultivation medium, the microorganism was not able to produce enough enzyme and enzyme activities were low. Similar effect was caused by high temperature. Highest values of enzyme activities were achieved at optimal fermentation conditions and the values were: 100.16-124.36 IU/ml (with sucrose as substrate for determination of enzyme activity) or 8.6-11.6 IU/ml (with inulin as substrate), respectively. The method of factorial design and response surface analysis makes it possible to study several factors simultaneously, to quantify the individual effect of each factor and to investigate their possible interactions (3). The model based on physiological assumptions is also applied. Assumed is a single enzyme rate determing growth (Monod kinetics) with proportional inulinase production rate. Applied are the models of reversible temperature and acidity inhibition based on thermodynamic equilibrium between active and inhibited enzyme states. Predictions by the two models are compared by ANOVA.
Inulinase Production by Kluyveromyces marxianus NRRL Y-7571 Using Solid State Fermentation
Applied Biochemistry and Biotechnology, 2006
Inulinase is an enzyme relevant to fructose production by enzymatic hydrolysis of inulin. This enzyme is also applied in the production of fructooligosaccharides that may be used as a new food functional ingredient. Commercial inulinase is currently obtained using inulin as substrate, which is a relatively expensive raw material. In Brazil, the production of this enzyme using residues of sugarcane and corn industry (sugarcane bagasse, molasses, and corn steep liquor) is economically attractive, owing to the high amount and low cost of such residues. In this context, the aim of this work was the assessment of inulinase production by solid state fermentation using by Kluyveromyces marxianus NRRL Y-7571. The solid medium consisted of sugar cane bagasse supplemented with molasses and corn steep liquor. The production of inulinase was carried out using experimental design technique. The effect of temperature, moisture, and supplements content were investigated. The enzymatic activity reached a maximum of 445 units of inulinase per gram of dry substrate.
Inulinase production by Kluyveromyces marxianus NRRL Y-7571 using solid state fermentation
Applied Biochemistry and Biotechnology, 2006
Inulinase is an enzyme relevant to fructose production by enzymatic hydrolysis of inulin. This enzyme is also applied in the production of fructo-oligosaccharides that may be used as a new food functional ingredient. Commercial inulinase is currently obtained using inulin as substrate, which is a relatively expensive raw material. In Brazil, the production of this enzyme using residues of sugarcane and corn industry (sugarcane bagasse, molasses, and corn steep liquor) is economically attractive, owing to the high amount and low cost of such residues. In this context, the aim of this work was the assessment of inulinase production by solid state fermentation using by Kluyveromyces marxianus NRRL Y-7571. The solid medium consisted of sugar cane bagasse supplemented with molasses and corn steep liquor. The production of inulinase was carried out using experimental design technique. The effect of temperature, moisture, and supplements content were investigated. The enzymatic activity reached a maximum of 445 units of inulinase per gram of dry substrate.
Microbial Inulinases: Fermentation Process, Properties, and Applications
Advances in Applied Microbiology, 1983
which can be used to synthesize useful compounds; others which can stereospecifically carry out important bioconversion reactions; and some which are able to hydrolyze polymers into interesting monomers. All these reactions can be performed under mild operating conditions with renewable resources as substrates (Rose, 1980). The microbial enzyme, inulinase, hydrolyzes the plant polymer inulin into practically pure fructose. Inulin accumulates as a reserve polymer in several interesting agricultural crops such as Jerusalem artichoke, chicory, scorzonera, dahlia, and several others. As such, inulinase offers interesting perspectives in view of the growing need for production of pure fructose syrups or may present an alternative way to produce the so-called "Ultra High Fructose Glucose Syrups" (UHFGS)not from starch, but from inulin. Conventional fructose production from starch needs at least three enzymatic steps, including a-amylase, amyloglucosidase, and glucose isomerase action, yielding only ? 45% fructose solutions at the best. Fructose formation from inulin is a single enzymatic step reaction and yields are up to 95% fructose. Indeed, fructose emerges more and more as a safe and alternative sweetener to sucrose, which causes problems related to corpulence, cariogenicity, atherosclerosis, and diabetes. Furthermore, fructose is more soluble than sucrose, displays a greater sweetening power, and can mask the bitter aftertaste of saccharin. Chemical acid hydrolysis of inulin to fructose displays several drawbacks; this has also forced interest toward the microbial inulinase enzyme and its applications. Another timely application of inulinase consists of the direct fermentation of inulin into ethanol with inulinase-producing yeasts. 11. History Lindner had already observed by 1900 that the yeast Saccharomyces mumianus, as well as a few unidentified yeast strains, were able to utilize inulin. Elaborations on these observations were reported by Grafe and Vouk (1913) and by Kluyver (1914), and Sacchetti (1933) unequivocally proved that Kluyveromyces fragilis grows at the expense of inulin. As for the molds, Aspergillus niger inulinase was first described in 1924 by Pringsheim and Kohn. Weidenhagen (1932) defended the opinion that a single enzyme-a pfructofuranosidase from yeast-hydrolyzed sucrose, inulin, rainose, irisine, gentianose, and stachyose. It was further claimed that only autolyzed yeast cells displayed inulinase activity. Until then, the existence of a true inulinase enzyme was strongly in doubt. Some years later, Adams et al. (1943) showed that even purified invertases from different yeast strains displayed varying inulinase activity depending upon the strain involved; it also became apparent that inulinase activity displayed a pH and temperature optimum quite
Inulinase Production from Plant Materials by some Local Yeast Strains
Journal of Agricultural Chemistry and Biotechnology, 2020
Four inulinolytic yeast isolates were isolated and identified by molecular methods. They were belonged to Candida catenulata, Sarocladium kiliense, Galactomyces candidum and Scopulariopsis brevicaulis. The production of inulinase by the previous isolates through, 72h was done on two media using jerusalem artichoke tubers, garlic bulbs, dahlia tubers and chicory roots as natural sources of inulin. The highest values of the produced inulinase were obtained on Bharathi et al. (2011) medium after 48h of incubation. Jerusalem artichoke proved to be the best inulin source for enzyme production by Scopulariopsis brevicaulis (38.22 µg/ml), followed by dahlia (32.45 µg/ml). The best substrate for inulinase production by Sarocladium kiliense was dahlia (29.21 µg/ml) followed by garlic (28.65 µg/ml). As for Galactomyces candidum the maximum enzyme production was obtained using jerusalem artichoke (34.96 µg/ml) followed by garlic (31.84 µg/ml). The best material for inulinase production by Candida catenulate (34.15 µg/ml) was dahlia, followed by garlic (31.40 µg/ml). It can be concluded that inulin containing plant materials could be employed as a carbon source for inulinase production, it offer advantage in comparison to purified substrate because it have low cost and high productivity.
Bioscience Journal, 2015
The optimization of growth conditions for the production of inulinase by Penicillium funiculosum cells were studied as well as the continuous production of the enzyme using immobilized cells. The highest amount of enzyme (163.5U/mL) was obtained when the producing cells were incubated for 96 hours at 27 o C and 200 rpm in a fermentation medium containing both inulin and peptone as sole carbon and nitrogen sources respectively. However, when the cells of the tested microorganism were adsorbed on different carriers, especially linen fibers, their production ability was also successfully maintained, to different extends, for seven successive batches. Moreover, commercially pure inulin is very expensive in only small quantities, this fermentation medium was later substituted by a crude inulin solution obtained from Jerusalem artichoke tubers ( Helianthus tuberosus) . The crude inulin juice was able to sustain inulinase production during the second batch cultivation of the P. funiculosum...
Optimization of inulinase production by solid-state fermentation in a packed-bed bioreactor
Journal of Chemical Technology & Biotechnology, 2010
BACKGROUND: This work is focused on inulinase production by solid-sate fermentation (SSF) using sugarcane bagasse, corn steep liquor (CSL), pre-treated cane molasses, and soybean bran as substrates in a 3-kg (dry basis) packed-bed bioreactor. SSF was carried out by the yeast Kluyveromyces marxianus NRRL Y-7571 and response surface methodology was used to optimize the temperature, air flow rate and initial mass of cells.
Review of inulinase production using solid-state fermentation
Annals of Microbiology, 2019
The purpose of the present study is to critically analyze the recent literature covering the production of inulinase enzyme from various sources by solid-state fermentation and discuss various approaches to increase its production in solid-state fermentation, purification, and its properties. The review deals with the solid-state fermentative production of inulinase production. Inulinases have many applications in industries, such as for the production of ultra-high fructose syrup, biofuels, lactic acid, citric acid, and single-cell oil. Solid-state fermentation (SSF) is more economic, requires smaller vessels, lowers water intake, reduces wastewater treatments, higher product yield, lesser chance of bacterial contamination, and lowers energy consumption. Furthermore, the crude products obtained from SSF can be directly used as the source of enzyme for biotransformation. Although many reports are available on a wide range of microbes which produces inulinases by SSF, it is important to isolate novel microbes for its production. Also, extensive research is going on to exploit unexplored sources for SSF. Higher yield of inulinases can be achieved by bioreactor modeling and proper monitoring of physical and chemical parameters in SSF.