Quantitation of alpha-fetoprotein and albumin messenger RNA in human hepatocellular carcinoma (original) (raw)

To analyze gene expression of a-fetoprotein (AFP) and AFP gene expression is shown during massive liver albumin in hepatocellular carcinoma (HCC), messenger necrosis 7 and in hepatocarcinogenesis. On the other RNAs (mRNAs) of these proteins in six human hepatoma hand, albumin gene expression begins with the initiacell lines and in 30 cases of HCC were quantitatively tion of liver organogenesis and increases gradually analyzed by competitive reverse transcription (RT) folwith development. Albumin transcription is known lowed by polymerase chain reaction (PCR). The tranto be modulated by various factors such as hormones, scriptional levels of both AFP and albumin genes in the acute phase reaction, extracellular matrix, heat, HepG2 and Huh 7 cell lines were 10 10 copies/mg RNA, in and serum colloid osmolarity. 12 Previous studies recontrast to approximately 10 5 copies/mg RNA in HLE and vealed the modulation of AFP gene transcription in HLF cell lines. AFP and albumin mRNA levels in three several hepatoma cell lines of rodents. 13,14 However, normal livers were 10 5 and 10 10 transcripts/mg RNA, respectively. In 30 cases with HCC AFP mRNA level in transcriptional levels of AFP and albumin genes in huneoplasm was 10 to 10 5 -fold enhanced as compared with man hepatocellular carcinoma (HCC) and the biological that of nonneoplastic portion, and correlated with sesignificance of the varied AFP transcription have not rum AFP level and tumor size (P õ .01). In contrast, been clarified yet.

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