Antigen Presentation in Extracellular MatrixInteractions of T Cells with Dendritic Cells Are Dynamic, Short Lived, and Sequential (original) (raw)

the minimum contact time between TCR and surfacebound cognate peptide-MHC complexes was established in the range of 20 hr (Iezzi et al., 1998). The minimum duration of TCR signaling required by naive T cells is lowered to 6 hr in the presence of mature DC (Lanzarzburg vecchia et al., 1999) or to 2 hr for preactivated T hybridoma cells in the presence of macrophages (Underhill et Josef-Schneider Str. 2 al., 1999). It has also been shown that interaction of the TCR with plate-bound cognate MHC molecules induces 97080 Wü rzburg Germany migratory arrest in T lymphocytes provided by LFA-1/ ICAM-1-mediated adhesion (Dustin et al., 1997). The † Institute of Immunology University of Witten/Herdecke transition from a migratory to a sessile state is a prerequisite for the formation of a tight and highly organized ‡ Department of Dermatology University of Mü nster interaction and signaling zone between T cell and APC, termed supramolecular activation cluster (SMAC) (Monks Germany et al., 1998) or "immunological synapse" (Grakoui et al., 1999). A central portion of the interaction plane contains TCR, CD3, CD4 or CD8, CD2, and CD28, as well as signal-Summary ing molecules (e.g., Lck, Fyn, Zap70, PKC, MEKK2), whereas an outer ring-like zone is enriched for adhesion Cognate interactions of naive T cells with antigenpresenting dendritic cells require physical cell-cell molecules LFA-1 and ICAM-1 (Monks et al., 1997, 1998; Shaw and Dustin, 1997; Dustin and Shaw, 1999; Pen-contacts leading to signal induction and T cell activation. Using a three-dimensional collagen matrix video-ninger and Crabtree, 1999; Schaefer et al., 1999).