Calibration of the HemoCue point-of-care analyser for determining haemoglobin concentration in a lizard and a fish (original) (raw)

Haemoglobin concentration ([Hb]) is measured for a wide variety of animal studies. The use of point-of-care devices, such as the HemoCue, is becoming increasingly common because of their portability, relative ease of use and low cost. In this study, we aimed to determine whether the [Hb] of blue-tongued skink (Tiliqua nigrolutea) blood can be determined accurately using the HemoCue and whether the HemoCue overestimates the [Hb] of reptile blood in a similar manner to fish blood. Additionally, we aimed to test whether ploidy affected [Hb] determined by the HemoCue using blood from diploid and triploid Atlantic salmon (Salmo salar). The HemoCue Hb 201 + systematically overestimated [Hb] in both blue-tongued skinks and Atlantic salmon, and there was no difference between calibration equations determined for diploid or triploid salmon. The overesti-mation was systematic in both species and, as such, [Hb] determined by the HemoCue can be corrected using appropriate calibration equations. Cite as: Andrewartha SJ, Munns SL, Edwards A (2016). Calibration of the HemoCue point-of-care analyser for determining haemoglobin concentration in a lizard and a fish. Conserv Physiol 4: doi:10.1093/conphys/cow006.