Nylon polyethyleneimine microcapsules for immobilizing multienzymes with soluble dextran-NAD for the continuous recycling of the microencapsulated dextran-NAD (original) (raw)

Biochemical and Biophysical Research Communications, 1978

Abstract

Semipermeable nylon polyethyleneimine microcapsules were prepared to contain a multi-enzyme system of yeast alcohol dehydrogenase (EC 1.1.1.1) and malic dehydrogenase (EC 1.1.1.37) together with a soluble immobilized coenzyme, dextran-NAD+. In the presence of the substrates ethanol and oxaloacetic acid dextran-NAD+ was successfully recycled within the microcapsules by the sequential reaction of the included enzymes. Recycling rates of 72 cycles per hour were achieved. The microcapsules were also used in a continuous flow shunt where the rate of recycling of dextran-NAD+ reaches a peak within 45 min. and remains at this maximal level throughout 4 hours of continuous reaction. The microcapsules have shown good storage stability, 50% of the activity was retained after 18 days of storage.

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