Human infection by Brucella melitensis: an outbreak attributed to contact with infected goats (original) (raw)
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American Journal of Infectious Diseases and Microbiology, 2016
Brucellosis is major zoonosis that affects health and economy in many parts of the world. Brucellosis remains an uncontrolled problem especially in several regions of high endemicity such as the Mediterranean, Middle East. Transmition of Brucellosis in humans is strongly related to contact with infected animals. Diagnosis of brucellosis is based on microbiological and serological laboratory test. Methodology: A cross-sectional study was conducted between January 2016 to June 2016 to estimate the seroprevalence of brucellosis in the small ruminants. A total of 315 blood samples collected from sheep, goats and human contacts with animals in the age from 15 to 58 yearswere tested by Rose Bengal test. Also, bacteriological examination was done for 171 available milk samples small ruminants (134 sheep and 37 goats), one sample of stomach and visceral content from aborted fetus of goat and five blood samples of human contact with a current history of fever. PCR was employed. Results: The ...
Isolation and Identification of Brucella Melitensis from Naturally Infected Goats
2008
Background: Infection with Brucella melitensis (B. melitensis) is one of the most important causes of abortion in goats and sheep, and also causes severe systemic disease in exposed humans. In Ethiopia, based on seroepidemiological studies, brucellosis is known to be endemic. However, there is little information on the isolation and molecular detection of Brucella species in small ruminants. Therefore, the present study was conducted in the Amibara district of Afar Region of Ethiopia to isolate and molecularly detect Brucella infection in small ruminants. Results: Out of the total 64 samples cultured, eight samples (five vaginal swabs and three milk) were positive for Brucella species based on colony morphology, growth characteristics, modified acid fast staining and biochemical tests results. Further identification using Brucella-ladder PCR method showed that four of the isolates (three from vaginal swabs and one from milk) from goats amplified fragments of 1071 bp, 794 bp, 587 bp, 450 bp and 152 bp in band size. The molecular result combined with the microbiological and biochemical characteristics of the isolates indicated that the isolates were strains of B. melitensis. Conclusion: The finding of this study could suggest economic and zoonotic significance of B. melitensis and warrants for the need for control strategies in livestock and creation of awareness in the pastoral communities on the safe consumption of foods of animal origin and avoidance of physical contact with aborted materials.
Current Science, 2021
Brucellosis is an occupational infectious disease affecting both animals and humans. It causes abortions, retention of placenta, stillbirth and infertility in small and large ruminants. It is considered as a disease of economic importance to the society engaged in animal husbandry. The present study was aimed to estimate the prevalence of brucellosis in goats exhibiting abortions, retention of placenta and still births and in occupationally exposed individuals, to type the circulating Brucella species in Central India and risk factors for its spillage to human host. Seroprevalence of 34.72% (RBPT), 33.33% (SAT and S-LPS ELISA) was recorded in goats and 2.38% (RBPT, SAT and S-LPS ELISA) in occupationally exposed individuals. The clinical samples revealed three Brucella melitensis isolates as confirmed by bcsp31, IS711 and AMOS PCR. Early diagnosis, general awareness, restricted animal movement, hygiene, secure handling and disposal of infectious waste is warranted.
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2009
Brucellosis is a zoonosis and an important bacterial disease of domestic animals and humans. The disease causes economic losses, and potentially threatens human health. Brucella can be transmitted to human from infected animals, especially consumption of contaminated milk or milk products more less contact with their carcasses 1. Brucella genus, six species of Brucella recognized currently, B. abortus and B. melitensis are responsible for diseases characterized by placentitis, abortion and infertility in cattle and sheep, respectively 2. Human infections are often due to B. melitensis, with only a few instances of B. abortus infection 3. B. melitensis is considered a major risk to human health due to the high virulence of the agent to man and traditional consumption of raw
Current Science, 2021
Brucellosis is an occupational infectious disease affecting both animals and humans. It causes abortions, retention of placenta, stillbirth and infertility in small and large ruminants. It is considered as a disease of economic importance to the society engaged in animal husbandry. The present study was aimed to estimate the prevalence of brucellosis in goats exhibiting abortions, retention of placenta and still births and in occupationally exposed individuals, to type the circulating Brucella species in Central India and risk factors for its spillage to human host. Seroprevalence of 34.72% (RBPT), 33.33% (SAT and S-LPS ELISA) was recorded in goats and 2.38% (RBPT, SAT and S-LPS ELISA) in occupationally exposed individuals. The clinical samples revealed three Brucella melitensis isolates as confirmed by bcsp31, IS711 and AMOS PCR. Early diagnosis, general awareness, restricted animal movement, hygiene, secure handling and disposal of infectious waste is warranted.
Seroprevalence of brucellosis among dairy farm workers in Mexico
Salud Pública de México, 2016
Objective. To describe the seroprevalence and associated factors for brucellosis among dairy farm workers. Materials and methods. We performed a secondary analysis of a data set and sera from a previous cross-sectional study in a dairy farm. Sera were tested for Brucella spp. antibodies by the slide agglutination test. Seropositivity was defined as a titer ≥1:40; recent infection was titers ≥1:160. Results. We tested 331 human sera. Seroprevalence of brucellosis was 18.1% (60/331; 95% CI 14.1-22.7); 13.3% of them (8/60; 95% CI 5.9-24.5) corresponded to recent infection. Highexposure occupation (calf caretaker; OR 3.3; 95%CI 1.1-9.7), daily hours in contact with cows (OR 1.1; 95%CI 1.03-1.2), and living on-site (OR 2.2; 95% CI 1.1-4.4) remained independently associated with seropositivity. Conclusions. We found a high seroprevalence of brucellosis among dairy farm workers, as well as a significant association among those with prolonged and close contact with cattle.
Seroprevalence of Brucella abortus and Brucella melitensis among
2013
Brucellosis is a widespread zoonosis mainly transmitted from cattle, sheep, goats, pigs and camels through direct contact with blood, placenta, fetuses or uterine secretions, or through consumption of contaminated raw animal products (especially unpasteurized milk and soft cheese). In endemic areas, human brucellosis has serious public health consequences. Worldwide, with an estimated 500,000 new human cases each year. Brucella melitensis the most prevalent species causing human brucellosis, owing in part to difficulties in immunizing free-ranging goats and sheep.Our research estimated the prevalence of brucellosis among butchers and determined the risk factor that increase the exposure toB.abortus and B.melitensisamong this study group in Thamar city from May to December 2012 . The present study is Cross-sectional study target approximately all butchers that work in butchery and shops of meat selling (64 butchers). Samples were examined by rose Bengal test (SAT) in General Thamar H...
Detection of Brucella melitensis in seropositive goats
Plumeriastuti H, Zamri-Saad M., Detection of Brucella melitensis in seropositive goats, Online J Vet Res, 16(1): 1-7, 2012. This study reports a relationship of Brucella melitensis with pathological changes in sero-positive goats. 1,054 serum samples were subjected to the Rose Bengal Precipitation Test (RBPT) of which 27 were positive. Serum samples were recollected from the 27 sero-positive goats 2 weeks later and subjected to RBPT for antibody titres. Uterii, mammary glands and supra-mammary lymph nodes were examined for pathological changes, immuno-peroxidase (IP) staining, bacterial isolation and PCR to detect B. melitensis. Results revealed that of the 27 goats tested, 41% were Category I (antibody titre = 2), 33% Category 2 (titre = 4) and 26% Category 3 (titre = >4). Goats in Categories 2 and 3 revealed more severe pathological changes with the latter being most severe. All goats in Category 3 showed positive reaction to IP compared with 56 and 18% in Categories 2 and 1, respectively. Three (33%) goats in Category 2 and 3 (43%) goats of Category 3 were B. melitensis positive either by isolation or PCR. There was strong correlation between the antibody titre, pathological lesions and presence of B. melitensis.