Resistin induces a disintegrin and metalloproteinase with thrombospondin motifs-4 gene expression in human chondrosarcoma cell line (original) (raw)

European Journal of Cancer

The irreversible destruction of extracellular matrix (ECM) components is key process for inflammatory and autoimmune diseases. A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS), a family of metallopeptidase involve in several physiological and pathological processes including degradation of matrix components. Resistin, an adipokine was characterized in 2001. The involvement of resistin in several biological processes was established before. However, the putative catabolic role of resistin on the components of ECM was not elucidated in detail. The aim of study was investigate to ADAMTS-4 gene expression level in SW1353 chondrosarcoma cell line after resistin induction. The SW1353 chondrosarcoma cell line was plated at 37 oC in a humidified atmosphere of 5% CO2. After confluent, the cell was stimulated by resistin at 100 ng/mL dose, and incubated for 6, 12, 24, and 48h. At the end of the stimulation, the total RNA was isolated, and reverse transcribed into cDNA u...

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