QTL analysis of resistance to sharka disease in the apricot (Prunus armeniaca L.) ‘Polonais’ × ‘Stark Early Orange’ F1 progeny (original) (raw)
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Evaluation of resistance of apricot cultivars to a Spanish isolate of plum pox potyvirus (PPV)
Plant Breeding, 2000
The resistance of 01 apricot cultivars to the Dideron type Spanish isolate RB2[29 of plum pox potyvirus was evaluated in controlled conditions in an insect!proof greenhouse[ The results of the evaluation demonstrated the resistance of the cultivars {Stella|\ {Stark Early Orange|\ {Goldrich|\ {Harcot|\ {NJA1|\ {Pandora| and {Avilara| to the isolate\ and the susceptibility of the cultivars {A1453|\ {Bu lida|\ {Currot|\ {Real Fino| and {Canino|
Genetics of resistance to Plum pox virus in apricot
Plant Protection Science
Plum pox virus (PPV) causes serious damage in apricots grown in the Czech Republic and other countries where it is<br />present. The virus spreads in orchards from infected trees by aphids to healthy trees of susceptible cultivars. Chemical<br />control is ineffective from epidemiological point of view. For this reason growing of resistant apricot cultivars is the only<br />way how to solve one of the most significant phytopathological problem. To study PPV resistance in apricot, three crosses<br />between an apricot cultivars or a selection resistant to PPV and an apricot cultivars or a selection susceptible to PPV<br />(LE-3218 × Stark Early Orange, LE-3241 × Vestar and LE-3246 × Vestar) were performed at Faculty of Horticulture<br />of Mendel University of Agriculture and Forestry in Lednice na Moravě in 1999. The BC1 seeds were stratified and the<br />subsequent seedlings were grown in a greenhouse. The seedlings were repeatedly inoculat...
2012
Sharka disease, caused by this virus (PPV) is one of the most serious viral diseases of stone-fruit crops, including peach (Prunus persica L.), apricot (P. armeniaca L.), plums (P. domestica L. and P. salicina Lindl.) as well as sweet and sour cherries (P. avium L. and P. cerasus L.) that may be systemically infected by a few unique PPV strains. The goal of this work is to evaluate a large number of local apricot varieties concerninhg the resitance to PPV, and using them on the valuable breeding programs, is an interesting perspective in limiting the spread of th is virus. In support of this idea we studied a large number of genotypes grafted on the mirobolan rootstocks and GF305 (considered indicator to PPV), that were previously artificial infected with PPV by chip budding. The rootstocks and the apricot varieties were tested by Elisa and RT-PCR.
Inheritance of resistance to Plum pox virus in apricot (Prunus armeniaca L.)
Tree Genetics & Genomes, 2007
The inheritance of resistance to Plum pox virus (PPV) has been studied in 1,178 apricot hybrids. Seven hundred and eighteen F1 hybrids, obtained from controlled crosses between the susceptible Greek cultivar "Bebecou" and the resistant PPV cultivars of American origin ("Stark Early Orange," 'NJA2," 'Veecot," "Sunglo," "Harlayne," and "Orangered") were evaluated for resistance to the PPV-M (Marcus) strain, 8 years after artificial inoculation. The inheritance of resistance to PPV has been additionally studied for the first time in a BC 1 population of 95 apricot hybrids for four vegetative periods. Reaction of each hybrid to PPV-M was scored through visual symptoms, indexing onto GF-305 and double-antibody sandwich enzyme-linked immunosorbent assay tests. Segregation within the hybrids, determined by Chisquared analysis, fits a 1:1 ratio (P≤0.05) of the resistant vs susceptible, indicating that resistance to PPV is controlled by a single dominant gene locus and that the above six resistant cultivars are heterozygous for the trait. Plants carrying this gene may initially develop disease symptoms on leaves but eventually recover and no virus can be detected in leaves. Susceptible plants show similar symptoms initially but remain symptomatic. Inheritance of resistance to PPV also has been studied in 365 F1 hybrids by crossing the resistant cultivar "Stella" with the susceptible "Bebecou" and the resistant cultivars "Sunglo" and "NJA2," for 8 years after inoculation. The segregation ratio was 1:0 (resistant/susceptible) suggesting that "Stella" is homozygous for the resistance trait. The purpose of this work was the enhancement of the knowledge of inheritance of resistance to PPV for the selection of new cultivars.
2005
Results of three years evaluation of resistance of apricot cultivars ‘Harlayne’ and ‘Betinka’ to the six different isolates of Plum pox virus (PPV) are presented. No symptoms appeared in the leaves of apricot cultivar ‘Harlayne’ in course of three years after inoculation of different strains of PPV. No symptoms appeared on the leaves of apricot cultivar ‘Betinka’ after inoculation of PPV-D (original), PPV-D (Prunus insititia), PPV-M (apricot), PPV-M (peach) and PPV-recombinant (PPV-M × PPV-D) strains. ELISA did not prove the presence of PPV strains in leaves of both cultivars with polyclonal PPV antibodies. Mild diffuse rings and spots appeared occasionally in the leaves of one tree out of three tested trees of apricot cultivar ‘Betinka’ after inoculation of PPV-M (original) in the third year of evaluation (2004). The presence of PPV was confirmed by ELISA. Symptoms of PPV, diffuse spots and rings, appeared on leaves of control cvs ‘Karola’ and ‘Velkopavlovická’. ELISA confirmed the...
2011
Breeding for fruit resistant to pests and diseases has become a major objective for many research laboratories. Excessive use of pesticides is increasingly denounced by consumers and the rules controlling their use (particularly with respect to toxic residues) are increasingly restricting. The use of resistant cultivars reduces production costs and increases workers safety. Prospecting through Romanian apricot collections has leaded us to the discovery of several sources of resistance to Sharka. The different Romanian hybrids or local apricot varieties was chosen among the different sources of resistance as it could also be used to develop a weeping variety in the some breeding program. To start with, the resistance mode of heritability was studied by creating F1 generations from the resistant parent crossed with a sensitive one, Mari de Cenad The resistance character is dominant and monogenic (symbols Rm1/m1). Breeding was continued by creating the F2 generations using by resistanc...
Testing genetic control hypotheses for Plum pox virus (sharka) resistance in apricot
Scientia Horticulturae, 2007
The resistance/susceptibility level of 213 descendants from three different crosses between the French apricot cultivar 'Polonais' (susceptible to Plum pox virus, sharka), and the North American cultivar 'Stark Early Orange' (resistant) was evaluated during four cycles of study under controlled greenhouse conditions. Resistant:susceptible ratios were 83:17 in the case of the 'Stark Early Orange' open-pollination descendants, 62:38 in 'Polonais' Â 'Stark Early Orange' descendants, and 28:72 in 'Polonais' Â 'Polonais'. These ratios were checked against the expected ratios of different genetic control hypotheses, combining one, two or three genes involved in the expression of this trait and with the resistance being a dominant (Hypothesis A) or recessive trait (Hypothesis B). In addition, two other, more complex hypotheses were proposed: that the resistance is controlled by 2 independent genes, the resistance being recessive for one and dominant for the other (Hypothesis C), and another hypothesis considering the resistance to be controlled by one dominant gene and admitting a 25% error in the evaluation process (Hypothesis D). The x 2 -test was applied to compare the goodness-of-fit of the proposed hypotheses. Hypothesis D seems to be the most consistent in the three apricot crosses studied. #
Agronomie, 2000
Information concerning the evaluation of apricot resistance to sharka (plum pox potyvirus) has in many cases been published in journals of limited circulation. In an attempt to assess the actual status of this resistance, a global review has been made, including 222 cultivars, 175 of which can be considered as susceptible, 27 as resistant and 20 as cultivars of uncertain classification. Such a doubtful classification could be due to differences in evaluation methods, the isolates used, or to the authenticity of the plant material. The results seem to indicate that the main origin of resistance to sharka could be North American cultivars, although other resistant sources have been found in Central Europe. Prunus armeniaca L. / apricot / plum pox potyvirus / sharka / resistance Résumé-Comportement des cultivars d'abricotier (Prunus armenica L.) à la sharka (plum pox potyvirus) : une revue. Les informations concernant l'évaluation du comportement de l'abricotier à la sharka sont fréquemment publiées dans des journaux à faible audience. La présente revue se propose de synthétiser ces informations dispersées : 222 cultivars sont pris en compte, 175 d'entre eux pouvant être considérés comme sensibles, 27 comme résistants et 20 pour lesquels la classification est incertaine. Cette classification douteuse peut être due à des différences dans les méthodes d'évaluation, aux isolats utilisés ou à l'authenticité du matériel végétal. Les résultats semblent indiquer que la principale source de résistance à la sharka aurait pour origine les cultivars nord-américains bien que d'autres sources de résistance ont été trouvées en Europe centrale.
Inheritance of resistance to plum pox potyvirus (PPV) in apricot, Prunus armeniaca
Plant Breeding, 2000
The results from the evaluation of apricot seedlings for resistance to the plum pox potyvirus "sharka# are presented[ The susceptibility of 180 seedlings from 19 di}erent crosses\ most of them between resistant and susceptible cultivars\ was determined[ The results obtained seem to indicate that the resistance to plum pox potyvirus in apricot is controlled by a single gene\ where resistance would be a dominant trait and the resistant parents used would be heterozygous for this trait[ Since the resistance appeared to behave as a quantitative trait during the evalu! ation process\ the hypothesis of a monogenic control is discussed[