Bradykinin b2 receptor expression in the bronchial mucosa of allergic asthmatics: the role of NF-kB (original) (raw)

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 2015

Abstract

Bradykinin (BK) mediates acute allergic asthma and airway remodeling. Nuclear factor-kappa B (NF-kB) is potentially involved in BK B2 receptor (B2R) regulation. In this observational cross-sectional study B2R and NF-kB expression was evaluated in bronchial biopsies from mild asthmatics (after diluent/allergen challenge) and healthy controls examining the role of NF-kB in B2R expression in primary human fibroblasts from normal and asthmatic subjects (HNBFb and HABFb). B2R and NF-kB (total and nuclear) expression was analyzed by immunohistochemistry in biopsies from 10 mild intermittent asthmatics (48 hours after diluent/allergen challenge) and 10 controls undergoing bronchoscopy. B2R co-localization in 5B5(+) and αSMA(+) mesenchymal cells was studied by immunofluorescence/confocal microscopy, and B2R expression in HABFb/HNBFb incubated with interleukin (IL)-4/IL-13 with/without BK, and after NF-kB inhibitor, by Western blotting. Bronchial mucosa B2R and nuclear NF-kB expression was h...

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