The morphological changes of adult mouse testes after 60Co gamma-Radiation (original) (raw)
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The morphological changes of adult mouse testes after 60Co γ-radiation
Iranian Biomedical …, 2008
Background: Cytotoxic therapy can lead to prolonged azoospermia or even sterility. In the present study, we investigated the morphological changes of mouse testes after γ-Radiation. Methods: After anaesthetizing of NMRI mice, testes and their surrounding tissues were irradiated using a cobalt therapy machine. Four experimental groups were irradiated with fractionated doses of: 1.5+8, 1.5+12 and 1.5+16 Gy (with an interval of 24 h) and single dose of 14 Gy. Non-irradiated mice were considered as control group. Testes were removed 4, 6 and 8 weeks following irradiation, weighed and processed for light microscopic study. Diameters of seminiferous tubules and their lumens, epithelium thickness, percentage of different types of tubules and number of spermatogenic cell were measured. Moreover, sperm count motility and viability rates were evaluated in epididymis. Results: Number of normal tubules, epithelium thickness, tubules diameter and lumen diameter were significantly reduced with high dose irradiation in comparison with control testes. The recovery was observed after 8 weeks. Epididymal sperm count, motility and viability rates were significantly decreased in the irradiated mice comparing non-irradiated ones. These parameters were increased after 8 weeks. Conclusion: According to the results, irradiation can cause temporary azoospermia in mouse and this effect is reversible after 8 weeks.
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1995
Radiation-induced perturbations in the steady-state spermatogenesis of mouse exposed to 0.05 to 2 Gy of 6"Co gamma-radiation were studied at 7 to 70 days post-irradiation flow cytometrically. Five quantifiable populations viz: elongated spermatids (HC), round spermatids (lC), spermatogonia and other diploid cells (2C), spermatogonial cells synthesizing DNA (S-phase) and primary spermatocytes (4C) were identified in the sham-irradiated controls. Exposure of mice to different doses of radiation resulted in a significant decline in the total germ-cell transformation ratio (I C:2C) at 2 1 and 28 days post-irradiation as compared to the control group, except for the animals exposed to 0.05 Gy. The lC:2C ratio is subdivided into two components viz. 4C:2C and lC:4C. The 4C:2C ratio decreased significantly on day 14 post-irradiation, except for 0.05 Gy where it was non-significant. Consequently, meiotic transformation (lC:4C) showed a significant increase on day 14 post-irradiation compared to the sham-irradiated control barring 0.05 Gy where the difference between the two groups was non-significant. The ratio of HC: IC (cell transformation during spermiogenesis) increased significantly at day 21 post-irradiation 0.2 to 2 Gy and between day 7 and 14 for 0.05 Gy as compared to the control group. It appears that a dose as low as 0.05 Gy radiation was able to cause the perturbations in the steady-state spermatogenesis of mouse and normalcy was not restored even up to 70 days post-irradiation at all exposure doses.
Current Issues in Molecular Biology
Background and purpose: With the emergence of linear accelerators in radiotherapy, it becomes necessary to accurately select new dosing regimens. The purpose of this study was to assess the morphological changes of spermatogenesis after radiation exposure. Materials and methods: Male Wistar rats (n = 40) were subjected to targeted ionizing radiation on a pulsed electron accelerator “NOVAC-11” with doses of 2, 8 and 12 Gy. Spermatogenesis was assessed a week later using light microscopy and immunohistochemical method (antibodies to Ki-67, Bcl-2, p53, Caspase 3). Results: A decrease in the number of normal germ cells was seen in all experimental groups, while radioresistant Sertoli and Leydig cells were barely affected. The most serious damage to the tubules and germ cells was observed in 8 and 12 Gy irradiation groups. IHC analysis of testes after irradiation showed a shift in the proliferative-apoptotic balance toward apoptosis of germ cells: a decrease in the expression levels of K...
Original Contribution EFFECT OF EXTERNAL GAMMA IRRADIATION ON RABBIT SPERMATOGENESIS
2006
Adult male rabbits were subjected to whole-body external gamma irradiation at 0.5 Gy, 1.5 Gy and 2.5 Gy in order to evaluate its effect on spermatogenesis. The quantitative and qualitative parameters (concentration of spermatozoa and percentage of pathological forms) of semen were determined between post irradiation days 10 and 60. The highest degree of spermatogenesis impairment was observed in rabbits irradiated at 2.5 Gy. Following increase of the challenge dose, a reduction in spermatozoa concentration in semen and increased percentage of pathological spermatozoa were observed. The cytogenetic analysis of irradiated spermatogonia showed a dose-dependent increase in the frequency of radiation-induced reciprocal translocations, manifested primarily as ring and chain configurations.
Quantitative analysis of radiation-induced changes in sperm morphology
PubMed, 1982
When developing spermatogenic cells are exposed to radiation, chemical carcinogens or mutagens, the transformation in the morphology of the mature sperm can be used to determine the severity of the exposure. In this study five groups of mice with three mice per group received testicular doses of X irradiation at dosage levels ranging from 0 rad to 120 rad. A random sample of 100 mature sperm per mouse was analyzed five weeks later for the quantitative morphologic transformation as a function of dosage level. The cells were stained with gallocyanin chrome alum (GCA) so that only the DNA in the sperm head was visible. The ACUity quantitative microscopy system at Lawrence Livermore National Laboratory was used to scan the sperm at a sampling density of 16 points per linear micrometer and with 256 brightness levels per point. The contour of each cell was extracted using conventional thresholding techniques on the high-contrast images. For each contour a variety of shape features was then computed to characterize the morphology of that cell. Using the control group and the distribution of their shape features to establish the variability of a normal sperm population, the 95% limits on normal morphology were established. Using only four shape features, a doubling dose of approximately 39 rad was determined. That is, at 39 rad exposure the percentage of abnormal cells was twice that occurring in the control population. This compared to a doubling dose of approximately 70 rad obtained from a concurrent visual procedure.
Abnormalities in the shape of murine sperm after acute testicular X-irradiation
Mutation Research: Fundamental And Molecular Mechanisms Of Mutagenesis, 1974
The fraction of murine epididymal sperm with shape abnormalities ranged from 1.o-15.o% in 14 inbred stlains and hybrids studied. The effect of X-rays on this fraction was examined in mice with markedly different natural levels of abnormalities, C57 BL (9.4%) and (C57BL × C3H)F1 (1.o%). Dosages of 30, IOO, and 300 tad produced a significant increase in the number of abnormal forms, especially from 3-8 weeks following irradiation. The fraction of abnormal sperm at 5 weeks following graded doses of radiation from 0-300 rad increased from control values to 30%. The data for the hybrid fitted a curve which increased as the 3/2 power of the dose and which gave a doubling in abnormalities compared with control at 28 tad. Sperm abnormalities may permit a simple quantitative assay for damage to the genetic material of the male germ line.
Reproductive Toxicology, 1998
The effect of teniposide (VM-26) 0.05 mg/kg body weight treatment on spermatogenesis of mice exposed to 0, 0.5, 1, 2, and 3 Gy gamma-radiation was evaluated flow cytometrically. Whole body irradiation with 1 to 3 Gy resulted in a significant decline in testis weight from Day 14 to 35 post-irradiation depending on the exposure dose. Treatment of mice with teniposide before irradiation advanced the decline in testicular weight by several days, especially at 3 Gy, where a significant decline in testicular weight was observed at Day 7 post-irradiation when compared with the double distilled water (DDW)؉irradiation group. The relative percentage of the 2C population declined significantly in the VM-26؉irradiation group in comparison with the DDW؉irradiation group at various post-irradiation time periods depending on the exposure dose. A significant depletion in the relative percentage of S-phase cells was observed as early as Day 1 post-irradiation in the VM-26؉irradiation group when compared with the DDW؉irradiation group after exposure to 1 to 3 Gy. This decline continued up to Day 21 post-irradiation after exposure to 2 Gy. The relative percentage of primary spermatocytes showed a consistent decline after exposure to various doses of gamma-radiation in the VM-26؉irradiation group when compared with the DDW؉irradiation group at different time periods, with a few exceptions, especially at higher doses. The pattern of decline in the relative percentage of round spermatids was similar to that of primary spermatocytes, where a significant decline was observed at various post-irradiation time periods in the VM-26؉irradiation group in comparison with the DDW؉irradiation group. These changes in the relative germ cell percentages are manifested as alterations in the ratios of various germ cell populations. The 4C:2C ratio declined consistently from Day 1 to Day 70 post-irradiation in the VM-26؉irradiation group at all exposure doses. Similarly, the 4C:S-phase ratio in the VM-26؉irradiation group also showed a significant decline at different post-irradiation time periods when compared with the DDW؉irradiation group depending on the exposure dose. The reduction observed in the relative percentages of various cell populations was higher in the combination group when compared with the DDW؉irradiation controls, indicating potentiation of damage to male germ cells by teniposide treatment before irradiation.
Gamma ray radiation effects on some sperm factors of male rats
2020
Introduction : This work studied the effect of Soft Gamma radiation with doses rate 9.1 16.2 and 36.4 mGy/h of exposure on male reproductive system of s albino rats. Materials and Methods :Twenty rats were used in the experiment ,they were randomly assigned into 4 groups of 5 animals for each group. White rats were used in this study albino rats Blub/c which range between 2-3 months of age and 170 -200 gm of weight . Healthy of these mice were obtained from the collage of veterinary in Mosul University.rats were exposed to 9.1,16.2 and 36.4 mGy/h with period of 7h/day for 90 days ,then rats were divided into 4 groups each of group contain 5 animals . group I : considered as control group were placed in cages with out exposure. group Π :Rats group were exposed to gamma ray with 9.1 mGy/h at period 7day/h for 90 days. group Ш : Rats of this group received 16.2 mGy/h at period 7day/h for 90 days. group IV : Rats of this group received 36.4 mGy/h at 7day/h for 90 days . After exposure t...
Effect of Gamma Radiation on Some Sperm Factors of Male Rats
Iranian Journal of Medical Physics, 2021
Introduction: The present study investigated the risks of ionizing radiation on sperm counts in chronic doses and compared the findings with previous results in similar and different conditions to minimize oxidant stress on sperm parameters rather than using black seed oil. Material and Methods: Twenty rats were used in experimental designs 1 and allocated unordered to four groups. Each group included five. The ranges of 2-3 months and 170 -200 g, respectively .The healthy rats were obtained from the University of Mosul ,Iraq .Experimental design 2, was conducted on 50 rats .The rats were exposed to three different doses for 30 days similar to those of experimental design 1. Oral black seed oil was administrated a dose of 20 mg/kg in group 2 . Results: In experimental design 1, there was a significant decrease in sperm count, live sperm percentage and normal sperm percentages respectively. However a significant increase was observed in dead sperm and abnormal sperm percentages in experimental design 1.The administration of black seed oil in excremental design 2 improved all the parameters with reducing abnormal and dead sperm counts rather than increasing normal and live sperm counts at all doses . Conclusion: The use of black seed reduce the oxidative stress caused by low dose gamma radiation .Therefore , this substance can be used as a therapeutic option for the treatment of several type of cancer especially those under the treatment of low dose gamma radiation through enhancement of protection for a long time.
Biodiversitas Journal of Biological Diversity
60 Co gamma ray exposure to the reproductive systems of rat (Rattus argentiventer) as sterile male technique. Biodiversitas 21: 3805-3810. Various strategies have been implemented to control pests, however, these do not able to reduce rat attacks on rice plants. Therefore, the sterile male technique (SMT) using 60 Co gamma radiation was introduced. A group of rats (Rattus argentiventer) of 2 months age were irradiated with 1-6 Gy doses (dose rate of 99.5631 cGy/min) and the quantity, morphology, and viability of spermatozoa of rats were assessed with standard procedures. The results showed that the concentration of spermatozoa was decreased with increasing radiation doses where for lower than 4 Gy were categorized as normal (>20 million/mL). The morphology of spermatozoa also decreased with increasing radiation dose where under 3 Gy were in normal (> 50%). Spermatozoa viability was also decreased where for doses 1 and 2 Gy were categorized as normal. Statistical analysis showed a significant difference (p<0.05) between the exposed group to control in spermatozoa quantity, morphology, and viability. This finding is quite similar with other experiments on the reproductive appearance of rat based on spermatozoa after irradiation. It is concluded that dose of 3 Gy that caused changes in spermatozoa with its viability still above 50% is the most appropriate dose for SMT of rice field rats.