Protective Effects of Fermented Citrus Unshiu Peel Extract against Ultraviolet-A-induced Photoageing in Human Dermal Fibrobolasts (original) (raw)
Related papers
2014
The aqueous extracts of Citrus unshiu peel containing flavonoid glycosides was used as co-substrate with Schizophyllum commune mycelia producing b-glucosidase and its biological activities were studied. b-glucosidaseproduced S. commune mycelia converted the glycosides (narirutin and hesperidin) into aglycones (naringenin and hesperetin). The photoprotective potential of fermented C. unshiu peel extract with S. commune (S-CPE) was tested in human dermal fibroblasts (HDFs) exposed to UVA. It was revealed that S-CPE had an inhibitory effect on human interstitial collagenase (matrix metalloproteinase, MMP-1) expression in UVA-irradiated HDFs. The treatment of UVA-irradiated HDFs with S-CPE resulted in a dose-dependent decrease in the expression level of MMP-1 mRNA. The UVA irradiation raised the proportion of senescence-associated b-galactosidase (SA-b-gal) positive cells in comparison with the normal control group. The treatment of UVA-irradiated HDFs with S-CPE was shown to decrease the level of SA-b-gal (by approximately 45% at an S-CPE concentration 0.1%, w/v) compared with the UVA-irradiated HDFs. It was found that S-CPE containing hesperetin has notable collagen biosynthetic activity for fibroblasts, indicating that S-CPE can be promising cosmetic ingredients.
The Effects of Helichrysum italicum Extract on the Extracellular Matrix of the Skin
Acta Medica Academica, 2023
Objective. An in-vitro study was performed to investigate the molecular basis of the wound healing and skin protective features of Helichrysum italicum (HI), a medicinal plant from the Mediterranean basin. Materials and Methods. A dermal fibroblast cell line culture was treated with HI hydro-alcoholic extract to detect the gene expression levels of three selected primers: FGF-2, HAS-2 and MMP-9. Cell proliferation assay was performed using a XTT reagent. RNA isolations were carried out from both the extract treated study cell group and the control cell group using a TRI reagent. GAPDH was used as the reference gene. Gene expressions were determined by real time RT-qPCR. The results were represented as 'Target/GAPDH Fold Change'. Statistical evaluation was performed by Student's t test. Results. HI extract caused statistically significant upregulation of FGF-2 (P=0.0473) and HAS-2 (P=0.0335) gene expressions compared to the untreated control cells. The treatment ended with 1.74 and 3.10 fold changes for FGF-2 and HAS-2, respectively. Conclusion. In general, it may be considered that HI has certain anabolic effects on the extracellular matrix of the skin because of the significant increases it causes in FGF-2 and HAS-2. Therefore, it may have a promising future in anti-aging studies and cosmetic dermatology. The results obtained in this study may also partially explain the molecular basis of the health benefits of HI on skin, including improvement in wound healing, and protection against the detrimental effects of ultraviolet exposure.
Pharmaceutical Biology, 2017
Context: The search for bioactive compounds from botanical sources is attracting much interest. However, differences in chemical composition may occur within the same species depending on different geographical origins. Objectives: We evaluated the properties on skin enzymes and cells of extracts from sulla legume crop Hedysarum coronarium L. (Fabaceae), collected at two Italian sites near Pisa and Ventimiglia, for possible dermatological and cosmetic applications. Material and methods: Plant aerial portions were extracted in MTBE/ethyl acetate/acetone, obtaining two extracts named Pisa sulla extract (PSE) and Ventimiglia sulla extract (VSE). Extracts were subjected to chemical characterization, LC-MS/MS analysis and biological assays. Results: PSE showed stronger antiradical scavenging and higher phenolic and flavonoid contents with respect to VSE. LC-MS/MS analysis revealed similar composition for the two extracts, but PSE was richer in condensed tannins and flavonoids, principally rhoifolin, quercetin, naringenin and derivatives. PSE induced stronger inhibition on collagenase and elastase by in vitro enzyme assays, possibly due to higher levels of condensed tannins and quercetin. ELISA bioassay on human dermal fibroblasts revealed stronger PSE induction of collagen production. Determination of glycerol release from adipocytes disclosed stronger stimulation of lipolysis by PSE, allegedly ascribed to higher charge of quercetin and derivatives. In summary, the higher richness in phenolics of PSE is strictly related to stronger bioactivity. Discussion and conclusions: Data indicate that aerial H. coronarium material is suitable for the development of dermatological and cosmeceutical products, but the geographical origin is an important factor for maximally exploiting the biological properties of this species.
Antioxidants and Skin Protection
Antioxidants, 2020
Natural products have a long history of use for skincare and the improvement of the appearance and function of aged and/or damaged skin. Among them, bioactive peptides, oligosaccharides, plant polyphenols, carotenoids, vitamins, and polyunsaturated fatty acids are the most widely used ingredients. In recent decades, natural products have undergone rigorous testing, resulting in the identification of phytochemical compounds such as antioxidants with important potentials for the development of cosmetics, cosmeceuticals, and nutraceuticals. Supplementation with these products has been shown to have an effect on the signs of ageing in several human trials. In this Special Issue, up to 12 original manuscripts and a review are included, covering most of the topics related to the use of natural compounds for skincare and protection. This editorial aims to highlight the most relevant contributions of each single manuscript to the current "state of the art" and provide global significance for the use of antioxidants in skincare. Agulló-Chazarra and co-workers [1] have investigated the use of an agriculture by-product to obtain potential ingredients for the cosmetic industry. They have used sweet cherry stems, a novel by-product that has hardly been explored until now. In addition, they have developed an interesting approach, using a multistep procedure covering several aspects of antioxidant and related bioactive properties to select the best extract and propose it as an interesting ingredient for the cosmetic industry. Benincasa et al. [2] have used another raw material to develop their study; in this case, they have studied the use of olive oil by-products to obtain a pure compound, hydroxytyrosyl oleate (HtyOle), and then study its antioxidant and regenerative properties on human keratinocytes. The use of by-products from agriculture is really interesting, because it aligns with the Sustainable Development Goals of the United Nations for 2030 [3] and promotes the use and valorisation of non-previously or low-used materials for high-value applications. The photoprotective properties of natural products has been addressed by five of the original articles of this Special Issue as their central topic [4-8]. These articles show up the relevance of photoprotection not only in cosmetics but also in cancer research, as UV radiation is the main cause of some melanoma subtypes. In the first of these articles, Kuo et al. [4] focused on the role of N-(4-bromophenethyl)-caffeamide (K36H), a propolis derivative, as a protective compound against UVA-induced apoptosis. This compound has showed its antioxidant activity by reducing reactive oxygen species (ROS) generation and decreasing metalloproteinase expression, DNA damage, and inflammation. The authors has also identified the main molecular mediators of all these actions and provided a global mechanism of how K36H acts inside human keratinocyte cells. A similar approach has been used by Wu et al. in their contribution to this Special Issue [8], testing the photoprotective and anti-inflammatory properties of a sesamin derivative on human fibroblasts. Tavares and colleagues has used mouse fibroblasts, human keratinocytes, and full-thickness reconstructed human skin to demonstrate the photoprotective actions of fucoxanthin [7]. Their results show that this compound is able to reduce ROS even when used in a prototype of final formulation
Journal of cosmetic science
As a part of ongoing research for novel natural cosmeceutical actives from plant extracts, this study demonstrates that Phyllanthus emblica fruit extract has shown its efficacy in protection against ultraviolet-B (UVB) irradiation-induced reactive oxygen species (ROS) and collagen damage in normal human dermal fibroblasts. At a concentration of 0.5 mg/ml, emblica extract showed a significant response of 9.5 ± 0.28-fold protection from UVB induced-collagen damage as compared to untreated cells. A known active, ascorbic acid, at a concentration of 0.5 mg/ml, showed 3.7 ± 0.07-fold protection from UVB-induced collagen damage. While the untreated cells showed 84 ± 1.4% induction in ROS on UVB irradiation as compared to the non-irradiated cells, emblica extract treatment inhibited the induction of ROS to 15 ± 4% at a concentration of 0.5 mg/ml. Ascorbic acid inhibited the induction in ROS to 64 ± 2% at a concentration of 0.5 mg/ml. Emblica extract is a significantly better natural active...
Anti-photoaging effect of fermented rice bran extract on UV-induced normal skin fibroblasts
European Food Research and Technology, 2010
Rice bran contains various polyphenolic compounds with anti-oxidative activities, and it has long been known to inhibit melanogenesis. Cofermentation of rice bran with Lactobacillus rhamnosus and Saccharomyces cerevisiae significantly reduced the melanin synthesis of the resulting extract to B16F1 melanoma cells. However, anti-aging and anti-inflammatory effects of these fermented rice bran extracts (FRBEs) were not investigated. The objective of this work was to investigate the ability of FRBE to protect fibroblast cultures against ultraviolet B (UV-B)-induced damage in vitro. To study the effect of FRBE on collagen synthesis, human fibroblasts were treated with 0.1, 0.5, or 1% of FRBE or 25 lM of As-2p over 3 days. The morphological change of fibroblasts, secretion of IL-1a, and pro-MMP-1 levels were evaluated. The rate of cell growth was reduced upon exposure of fibroblasts to UV-B radiation (30 mJ/cm 2 ) and did not recover after FRBE treatment. FRBE did not induce morphological changes and necrosis in a dose-dependent manner.
Potentials of plant derived products for the treatment of skin disorders
German Journal of Pharmaceuticals and Biomaterials
The skin is the body's largest organ. The epidermis and dermis make up the skin, and their primary purpose is to defend the body from adverse environmental factors like chemicals, allergens, toxins, and bacteria. Many different types of natural products have shown promise in the treatment of skin disorders. Multiple synthetic chemicals and environmental pollution have an impact on modern human existence. Therefore, nature has provided several essential ingredients for boosting skin health and shielding skin from environmental damage. The most significant in vivo and in vitro studies on the use of different natural products in inflammatory, cancerous, and skin infection disorders and their mechanisms of action were summarised in this review. The study also highlights the potential photoprotective effects of numerous herbal ingredients. Sunburn is caused primarily by the sun's ultraviolet rays, which can also cause cancer of the skin. Herbal ingredients with sunblocking properties can prevent the penetration of harmful ultraviolet radiation. Compared to their synthetic counterparts, herbal remedies have fewer adverse effects and are just as effective in treating chronic conditions. Flavonoids, polyphenols, carotenoids (Lycopene, carotene), and phenolic acids found in herbs rich in vitamins (A, C, and E) have antioxidant properties that increase photoprotection.
Malaysian Journal of Science
Carrageenans, the polysaccharide from red seaweeds is widely used in food, medicine and as an excipient in cosmetics and skincare products. Carrageenans have shown prospective photoprotective effect against ultraviolet B (UVB) irradiation on immortalised normal human keratinocyte (HaCaT) cells. The aim of this research was to evaluate the photoprotective effect of iota (ι), kappa (κ)-carrageenans and their combination with vitamin E against UVB-induced extracellular matrix (ECM) damage in HaCaT cells. The study also assessed the superoxide dismutase (SOD) and catalase (CAT) antioxidant enzymes in UVB exposed pre-treated and non-pre-treated cells. The parameters assessed were biological mediators important for the structural integrity of ECM, comprising of epidermal growth factor receptor (EGFR), mitogen-activated protein kinase (MAPK), activating protein- (AP-1) and matrix metalloproteinases (MMPs), liberated by the UVB exposed cells. Enzyme linked immunosorbent assay was used to ev...
Biotechnology and Bioprocess Engineering, 2012
Sea buckthorn (Hippophae rhamnoides L.) seed, which has very high nutraceutical, cosmetoceutical and therapeutic properties, has been widely used to treat diseases in Tibetan and Mongolian traditional medicines. In this study, we measured the antioxidant activities of the solvent-dependent SBS (Sea buckthorn seed) extracts using the DPPH and Folin-Ciocalteu assays and we investigated the efficacy of SBS in protecting skin against UVB-induced damage using cultured human dermal fibroblasts. In addition, the inhibitory effects of SBS on UVB-induced skin photoaging were examined by determining the level of Metalloproteinase (MMP)-1 and procollagen after UVB-irradiation. The antioxidant capacity of the SBSE (alcoholic) extract was significantly higher than the SBSH (hexane) and SBSW (water) extracts, as measured based on the free-radical scavenging activity and total phenolic content. SBSE was chosen as the most suitable candidate antioxidant. In human dermal fibroblasts, the cell viability of SBSE extract at doses of 2.5, 5, and 10 µg/mL was higher than the UVB-treated control. By RT-PCR and Western blot, SBSE treatment inhibited UVBinduced IL-1β expression in cultured cells. In addition, SBSE restrained UVB-induced IL-6 and COX-2 gene expression in cultured fibroblasts in a dose-dependent manner. Treatment with the SBSE extract after UVB irradiation in human dermal fibroblasts significantly reduced MMP-1 expression and increased procollagen synthesis when compared with UVB-irradiation only. In this study, SBSE was shown to increase the synthesis of procollagen, decrease the expression of MMP-1, and inhibit the production of IL-1β, IL-6, and COX-2 in UVB-irradiated human fibroblasts. These findings suggest that the SBSE extract may be a potential therapeutic agent for preventing and treating skin photoaging.
Anatomical Sciences Journal, 2018
Introduction: Hedera helix is an evergreen plant used in treatment of wounds in the Iranian folk medicine. One of the main stages in wound healing process is, the proliferative phase in which fibroblasts proliferate within the wounds and produce extracellular matrix. Collagen is the main constituent of extracellular matrix. So the aim of this study was to evaluate the effect of Hedera helix alcoholic extract on dermal collagen bundles in wound healing process. Methods: 7 adult Oryctolagus Caniculus rabbits were enrolled in this experiment. Four wounds were created on each rabbit. Alcoholic extract of hedera helix dried leaves were prepared. Wounds were treated with eucerin, phenytoin, hedera helix 10% and 20% eucerin-based creams for 20 days. On day 21 (after the completion of the experiment), skin samples were taken and the collagen bundles in reticular and papillary layers of the dermis were evaluated histologically. Results: Histological assessment of dermal collagen bundles in the reticular layer showed that both 10% and 20% hedera helix creams, increased collagen bundle thickness significantly, compared to eucerin treated wounds (P<0.001 and P<0.01). In papillary layer there was no significant effect in both groups. Compared to eucerin treated wounds, collagen bundles were more organized in phenytoin and hedera helix 10% and 20% cream treated wounds, in a Histomorphological point of view. Conclusion: Our results showed that hedera helix contains phytochemicals which may act on the proliferative phase of wound healing process.