Cellular and Viral Markers in Human Papillomavirus Related Cervical Lesions Progression (original) (raw)
The etiological role of high-risk human papillomavirus (HPV) in invasive cervical cancer has been demonstrated by epidemiological and molecular studies. In HPV-associated cervical carcinomas, the expression/ function of p16 is impaired by the action of viral E6 and E7 oncoproteins. In order to evaluate the potential of p16INK4a and E4 HPV16 mRNA as clinical markers for progression and development of cervical malignant lesions we analyzed cervical specimens from patients with different cytology (collected in liquid cytology medium from 89 women, (22-72 years old) which were used to isolate genomic DNA (High Pure PCR Template, Roche Diagnostics) and RNA (RNAeasy kit-Qiagen). E4 HPV16 mRNA gene expression was performed using E4 HPV16 PCR and the results obtained were confirmed by Southern Blot Hybridization. We evaluated p16INK4a expression using TaqMan Real Time PCR (Applied Biosystems). The molecular detection of HPV DNA and E4 HPV16 expression were corroborated with p16INK4a express...
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