Immunogenicity of Salmonella pullorum killed vaccine in selected breeder flock (original) (raw)
Related papers
Investigation on the Efficacy of a Killed Salmonella pullorum Vaccine
Microbes and Health, 2013
This study was carried out to determine the efficacy of a formalin-killed alum-precipitated Salmonella pullorum vaccine prepared by the Livestock and Poultry Vaccine Research and Production Centre (LPVRPC), BAU, Mymensingh, Bangladesh. Immunization with this vaccine induced serum antibody titers that peaked in 2-week following both primary and booster vaccination (P<0.05), and started to decline following 4-week of both vaccinations. Both primary and booster vaccination induced detectable antibody responses that were able to react with whole cells S. pullorum as determined by passive haemagglutination test (PHA). Both vaccinated chicken and mice showed maximal resistance following challenge with a virulent isolate of S. pullorum (P<0.01). In differential leukocyte counts in mice, a significant increase of lymphocytes was observed after primary immunization (P<0.01). Sera from vaccinated chickens conferred superior protection over naive chickens from lethal challenge with S....
Journal of Advanced Veterinary and Animal Research
Objective: The research work was conducted to determine the duration of protective efficacy and lowest immune protective titer of Salmonella bivalent vaccine containing Salmonella gallinarum and Salmonella pullorum prepared at the Livestock and Poultry Vaccine Research and Production Centre (LPVRPC) of Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh. Materials and methods: The experimental chickens were subdivided into four main groups (A, B, C and D). Group A and B were vaccinated with BAU-Salmonella bivalent vaccine with dosed 0.5 mL intramuscularly at the age of seven weeks followed by a booster vaccination at 12 weeks of age while group C and D served as unvaccinated control. The sera samples were obtained at 7, 12, 15, 18, 23, 27, 30, 32, 34, 36 and 41 weeks of age of birds. Results: Significantly elevated level of immune response in terms of antibody production resulted from booster vaccination. Vaccinated chicken showed protective resistance following virulent challenge with isolates of S. gallinarum and S. pullorum (**P<0.01) till 41 weeks, whereas unvaccinated control birds failed to resist the virulent challenge infection. Conclusion: BAU-Salmonella bivalent vaccine showed lowest immune protective titer up to seven months following booster vaccination.
Immunogenicity studies of various experimental vaccines in chickens
2020
In this paper, the main objective was to raise chickens' antibodies against three crucial public health microorganisms: the human immunodeficiency virus-1, Salmonella spp, and Staphylococcus aureus. Immunogens were prepared from the said microorganisms. Chickens were vaccinated either orally or intramuscularly. After a booster immunization, mostly eggs were collected and assess for the presence of specific antibodies. The most important results were the production of a large amount of anti-HIV antibodies in chicken's eggs, and also the synthesis of anti-protein A antibodies with the ability to inhibit the growth of S. aureus in vitro and to serve as anti-anti-idiotypic antibodies with the capacity of neutralizing the original antigen. Enzymelinked immune absorbent assays detected the presence of these antibodies as anti-Salmonella antibodies that were critical in reducing the bacterial load in the stomach and caeca compared with a control group. The vaccines were effective and safe, but more laboratory work, and economics have to be carried out to start a human trial.
Preparation and Evaluation of Chemically Inactivated Salmonella Enteritidis Vaccine in Chickens
Asian Journal of Pharmaceutical and Clinical Research, 2017
  Objective: Salmonella enteritidis ghosts (SEGs) is a non-living empty bacterial cell envelopes which were generated using a different concentration of sodium hydroxide (NaOH) 6.4 mg/mL and evaluated as a vaccine candidate in specific pathogen-free (SPF) chicken. SEGs have been produced by chemical-mediated lysis and evaluated the potential efficacy of chemically induced SEG vaccine and its ability to induce protective immune responses against virulent S. enteritidis challenge in SPF chickens.Methods: SPF chickens were divided into three groups: Group A (non-vaccinated control), Group B (vaccinated with prepared vaccine), and Group C (vaccinated with commercial vaccine).Results: Vaccination of SPF chicken with SEGs induced higher immune responses before and after virulent challenge. SPF chicken vaccinated with SEGs showed increasing in serum enzyme-linked immunosorbent assay (ELISA) antibodies. During the vaccination period, Groups B and C showed higher serum antibody titer compa...
Avian Pathology, 2010
An autologous killed trivalent vaccine (3)10 8 colony-forming units [CFU]), based on three Salmonella serovars (Typhimurium Á serogroup B, Mbandaka Á serogroup C, and Orion Á serogroup E) prevalent in the flocks of Australian poultry companies, was developed using Salenvac † techniques. At 20 weeks, hens vaccinated at 12 and 17 weeks as well as non-vaccinated hens were challenged (250 ml of 10 7 CFU) with autologous and heterologous serovars belonging to serogroup B (Typhimurium and Agona), serogroup C (Mbandaka and Infantis) and serogroup E (Orion and Zanzibar). Overall, vaccination resulted in a significant difference in carriage of Salmonella between non-vaccinated and vaccinated commercial Cobb hens (PB0.05) for serogroups B and C. However, due to low colonization rates in the non-vaccinated birds, no significant difference (P 0.05) could be determined for serogroup E. All vaccinated flocks produced a significant antibody response (PB0.001) to the S. Typhimurium vaccine strain, measured using a S. Typhimurium enzyme-linked immunosorbent assay (Guildhay), which peaked at 20 weeks of age, with 39% of the hens positive. Maternal antibodies were detected in 16% of the yolks from eggs produced by these flocks. There was a significant difference after challenge with Salmonella (PB0.05) among 1-day-old chicks from vaccinated versus non-vaccinated parents, when challenged using 10 4 CFU but not when challenged with 10 8 CFU. The success of this trial resulted in the incorporation of this vaccine into a Salmonella control system in commercial broiler breeder production.
In vitro Studies of Chicken Egg Yolk Antibodies Generated against Salmonella pullorum
Abstract: The present investigation is focused to generate chicken Egg yolk antibodies against Salmonella pullorum and their in-vitro characterization. Pullorum disease is leading cause of morbidity and mortality in poultry and highly responsible for significant economic loss. Mortality in such outbreaks may approach 90% if untreated. Treatment primarily is a salvage operation and does not prevent from becoming a carrier. Therefore, the prevention of this disease in breeder level through vaccination is more convenient for the control of vertical transmission. One of the recent researches has revealed that the combination of IBDV vaccine and chicken IgY generated against IBDV was superior in preventing IBDV infection in Broiler chickens rather than using them alone. Based on this recent finding, the chicken egg yolk antibody (IgY) raised against Salmonella pullorum. IgY antibodies were purified by (Polson et al., 1980) method and Water dilution method followed by DEAE cellulose ion exchange column chromatography. The total IgY concentration was relatively constant, average IgY concentration was 6.62 mg/mL during the immunization period. Titre of IgY antibodies was 1:10000 on 120th day after first immunization determined by ELISA. The agglutination was observed in both Rapid Slide Agglutination and Micro-titre plate (up to 1:2048 dilutions). It indicated the presence of IgY against S. pullorum. Present study concluded that the generated IgY was specific against S. pullorum whole cell antigen and it could effectively bind with that. The raised antibodies could be used for the passive immunotherapy to protect the young chicks from horizontal transmission of Pullorum disease by improving the immunological strength against infectious disease.
Efficacy of locally prepared Salmonella Kentucky vaccine in chicken
A B S T R A C T In the present study, efficacy of a locally prepared Salmonella Kentucky killed vaccine had been studied. A total of 120, two weeks old specific pathogen free (SPF) chicks were divided into two groups; 60 chicks each. First group was vaccinated with the prepared vaccine at the age of two weeks and boostered at four weeks, the second group was kept unvaccinated as a control group. The two groups were challenged orally with 1 ml of Salmonella Kentucky (5x10 7 CFU/ml), 3 weeks post boostering of the vaccine. The degree of protection was assessed according to the severity of the clinical signs, the mortality and fecal shedding of the challenged organisms. Blood samples were collected weekly after first vaccination till fourth week after challenge and humoral immune response was measured against Salmonella strains using ELISA and microagglutination test. The prepared vaccine induced 80% protection rate in challenge test with reduced fecal shedding. (http://www.bvmj.bu.edu.eg) (BVMJ‐29(2): 153‐160, 2015)
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2008
The experiment was performed in order to stimulate anti immunoglobulins (Ig) Salmonella gallinarum-pullorum production in Rhode Island laying hens. 4 types of antigen obtained by ultrasounds treatment with and without adjuvant were used for stimulation. The hens were inoculed following an hyperimmunization protocol and after this, the total Ig and anti Salmonella gallinarum-pullorum antibody in serum and vitelus was determined. Total Ig in serum raised from 5 to 6 Vernes degrees by the end of the experiment, and the vitellin ones reached a maximum level at 28 days, decreasing after. Serum and vitelus specific antibodies raised with the innoculum number by the end of the experiment. The highest values were obtained in groups hyperimmunized with ultrasound treated antigens. High specific Ig release by vitelus is considered an aspect to be used in salmonelosis prophylaxis in hens and other species.
Brazilian Journal of Microbiology, 2008
Salmonella Enteritidis is one of the agents that is responsible for outbreaks of human foodborne salmonellosis caused by Salmonella Enteritidis and is generally associated with the consumption of poultry products. Inactivated Salmonella Enteritidis cell vaccine is one of the available methods to control Salmonella Enteritidis in breeders and laying hens, however results in terms of efficacy vary. This vaccine has never been tested in Brazil, therefore, the present work was carried out to assess three commercial inactivated Salmonella Enteritidis vaccines allowed in Brazil. Four hundred white light variety commercial laying hens were obtained at one-day-of age. At eight weeks old, the birds were divided into four groups with one hundred animals each. Birds from three groups (V1, V2 and V3) received different intramuscular vaccines, followed by a booster dose at 16 weeks of age. Birds from another group (CG) were not vaccinated. When the laying hens were 20, 25 and 31 weeks old, 13 from each group were transferred to another room and were challenged by inoculating 2 mL neat culture of Salmonella Enteritidis. On the second day after each challenge, the caecal contents, spleen, liver and ovary of three birds from each group were analyzed for the presence of Salmonella Enteritidis. Twice a week a cloacal swab of each bird was taken and all eggs laid were examined for the presence of Salmonella Enteritidis. After four consecutive negative cloacal swabs in all the groups, the birds were sacrificed so as to examine the liver, caecal contents and ovaries. Overall, the inactivated vaccine used in group V3 reduced Salmonella Enteritidis in the feces and eggs. A very small amount of Salmonella was found in the spleen, liver, ovary and caeca of the birds in the four groups during the whole experiment. In general, inactivated Salmonella Enteritidis vaccines was able to decrease the presence of Salmonella Enteritidis in the birds and in the eggs as well. Nevertheless, they must be associated with general hygiene and disinfection practices in poultry husbandry.
Journal of Bacteriology & Mycology: Open Access
Background: Infection with Salmonella species is a major health concern for human and animals on a global scale. Most cases of Salmonellosis results in complicated diarrhea, elderly and immune-compromised persons can be at risk for more severe invasive infections which can be life threatening. Control of Salmonellosis in poultry by vaccination is a possible means of controlling the problems. Material and method: Two different inactivated trivalent S. Enteritidis, S. Typhimurium and S. Kentucky vaccine batches of 2 different origins were used to vaccinate salmonella free chickens with either single dose or single then booster dose vaccination programs. These chickens were reared in clean separated pens and later on were challenged with virulent S. Enteritidis, S. Typhimurium and S. Kentucky virulent strains 3 weeks post single or booster doses. Then protective indices was estimated as mean of vaccine evaluation. Results: Vaccinated birds showed varied protection according to challenge strains, vaccination program and origin of vaccine. Protective indices was estimated as 71% and 66.2% for local and commercial inactivated trivalent salmonella vaccine respectively when chicken challenged 3 weeks post single dose vaccination. Protective indices raised up to 82.5% and 79.5% respectively when birds challenged 3 weeks post booster vaccination. Conclusion: Evaluation of the combined salmonella vaccine depending on protective indices is more obvious and the picture more better than evaluation depending on either measurement of humoral response or mortalities post challenge because of protective indices depends on several parameters reflecting the immune status of the birds including mortalities , clinical signs and post mortem lesions.