Perspective Platelet-rich plasma: a healing virtuoso (original) (raw)
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Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 2009
Objective. To investigate the potentially useful of platelet-rich plasma (PRP) on mRNA expression of angiogenesis. Study design. Adjunct assay and reverse-transcription polymerase chain reaction (RT-PCR) analysis of type I collagen, vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGF) in rat bone marrow stromal cells differentiation in 14 days' culture. Results . The PRP significantly elevated alkaline phosphatase activity after day 5 (P Ͻ .05), and DNA and protein content increased at culture days 1, 3, and 5 (P Ͻ .01) with PRP compared with control. The RT-PCR demonstrated that type I collagen was expressed in all subtrates and remained high with PRP during 14 days of culture, and that mRNA expression of VEGF and PDGF were higher over time.
Platelet‐Rich Plasma: Growth Factors and Pro‐ and Anti‐Inflammatory Properties
Journal of Periodontology, 2007
Background: Platelet‐rich plasma (PRP) promotes regeneration of bone, presumably through the action of concentrated growth factors. However, it is not clear how PRP affects the inflammatory response. The purpose of this study was to analyze the growth factors in PRP and to study the effects of PRP on monocyte cytokine release and lipoxin A4 (LXA4) generation.Methods: PRP was prepared from healthy donors. Platelet‐derived growth factor (PDGF)‐AB, PDGF‐BB, transforming growth factor‐β1, insulin‐like growth factor‐I, fibroblast growth factor‐basic (FGF‐b), epidermal growth factor (EGF), vascular endothelial growth factor, interleukin‐12 (p40/70), and regulated on activation, normal T‐cell expressed and secreted (RANTES) levels were evaluated by enzyme‐linked immunosorbent assay and bead‐based multiplexing. Peripheral blood monocytes were isolated and cultured with or without PRP. Cytokine, chemokine, and LXA4 levels as well as monocyte chemotactic migration were analyzed.Results: Growt...
Role of platelet-derived growth factor in wound healing
Journal of Cellular Biochemistry, 1991
Platelet-derived growth factor (PDGF) in vitro stimulates DNA synthesis and chemotaxis of fibroblasts and smooth muscle cells and stimulates collagen, glycosaminoglycan, and collagenase production by fibroblasts. These in vitro properties suggest that PDGF, delivered by platelets to the site of injury in vivo, may play an important role in the
Growth-promoting action and growth factor release by different platelet derivatives
Platelets, 2013
Platelet derivatives are commonly used in wound healing and tissue regeneration. Different procedures of platelet preparation may differentially affect growth factor release and cell growth. Preparation of platelet-rich fibrin (PRF) is accompanied by release of growth factors, including platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF) and transforming growth factor b1 (TGFb1), and several cytokines. When compared with the standard procedure for platelet-rich plasma (PRP), PRF released 2-fold less PDGF, but 415-fold and 42-fold VEGF and TGFb1, respectively. Also, the release of several cytokines (IL-4, IL-6, IL-8, IL-10, IFNg, MIP-1a, MIP-1b and TNFa) was significantly increased in PRF-conditioned medium (CM), compared to PRP-CM. Incubation of both human skin fibroblasts and human umbilical vein endothelial cells (HUVECs) with PRF-derived membrane (mPRF) or with PRF-CM enhanced cell proliferation by 42-fold (p50.05). Interestingly, PRP elicited fibroblast growth at a higher extent compared to PRF. At variance, PRF effect on HUVEC growth was significantly greater than that of PRP, consistent with a higher concentration of VEGF in the PRF-CM. Thus, the procedure of PRP preparation leads to a larger release of PDGF, as a possible result of platelet degranulation, while PRF enhances the release of proangiogenic factors.
The therapeutic basis of platelet-rich plasma use in medicine is derived from the growth factor content and provisional matrix provided by the platelets themselves. This chapter briefly reviews the platelet research which led to the conceptual development of PRP as a treatment and also the early history of its use. An overview of platelet structure and function is provided to enhance the clini-cian's understanding of the cell biology behind PRP therapy. The 2 major growth factors in PRP (PDGF and TGFb) are also discussed. Finally, a review of the experimental PRP literature (in vitro and animal studies) is presented, which describes the evidence for use of PRP in tendon/ligament, bone, and joints. Standardization of PRP use remains a challenging prospect due to the number of variables involved in its preparation and administration. It may be that individually tailored PRP protocols are actually more beneficial for our patients—only time and further research will bear this out. Origins and Overview of PRP Use in Medicine As recently as forty years ago, platelets were considered to be exclusively hemostatic cells. Today we know that platelets actually perform myriad diverse functions. The conventional paradigm of limited platelet function began to shift in 1974, as the pathogenesis of atherosclerosis was beginning to be unraveled. Researchers studying the proliferation of smooth muscle cells in the vascular intima knew that 10 % serum was crucial to support cell growth in culture, but did not know which component of serum was responsible for the observed anabolic
International journal of implant dentistry, 2016
The development of platelet-rich fibrin (PRF) drastically simplified the preparation procedure of platelet-concentrated biomaterials, such as platelet-rich plasma (PRP), and facilitated their clinical application. PRF's clinical effectiveness has often been demonstrated in pre-clinical and clinical studies; however, it is still controversial whether growth factors are significantly concentrated in PRF preparations to facilitate wound healing and tissue regeneration. To address this matter, we performed a comparative study of growth factor contents in PRP and its derivatives, such as advanced PRF (A-PRF) and concentrated growth factors (CGF). PRP and its derivatives were prepared from the same peripheral blood samples collected from healthy donors. A-PRF and CGF preparations were homogenized and centrifuged to produce extracts. Platelet and white blood cell counts in A-PRF and CGF preparations were determined by subtracting those counts in red blood cell fractions, supernatant ac...
Orthopedic Research and Reviews
Platelet-rich plasma (PRP) contains many growth factors, such as FGF, which induces the production of type I collagen, and VEGF, which induces neovascularization, all of which are important in bone healing. This study aimed to evaluate the effect of PRP administration on type I collagen production, VEGF expression, and neovascularization in rat models following femoral bone implants using K-wire. Methods: An experimental randomized control study was conducted on 24 white male rats (Rattus norvegicus) in the Wistar strain that underwent K-wire implantation, where PRP was administered to the treatment groups. The amount of type I collagen was measured by immunohistochemistry VEGF expression using sandwich ELISA, and neovascularization by histopathological examination. Results: The amount of type I collagen in the treatment group (50->150/field of view) was significantly higher than the control group (0-99/field of view; p=0.003). VEGF expression in the treatment groups was significantly higher than controls: 10.90±4.47 and 2.29 ±0.92, respectively (p=0.006). Mean number of new vessels formed on fibrotic capsules in the treatment groups was significantly (p=0.007) higher than the control groups (2.69±1.03 vs 0.67±0.52). Conclusion: The use of PRP significantly increased type I collagen production, VEGF expression, and neovascularization in rat models, elucidating the potential of PRP to be used in clinical settings to enhance the bone-healing process.