Candida olivae sp. nov., a novel yeast species from 'Greek-style' black olive fermentation (original) (raw)
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Biochemical and Molecular Characterization of Some Yeast Isolates [ 27 ]
2018
Sixteen yeast isolates from various sources including molasses, foodstuffs, concentrated juices and soils were identified using simplified API 20 C AUX yeast identification system and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The isolates were found to belong to 11 species; Candida famata, C. glabrata, C. krusei / inconspicua, C. norvegensis, C. colliculosa, Cryptococcus humicolus, Rhodotorula mucilaginosa, Kloeckera spp., Geotrichum capitatum and Saccharomyces cerevisiae. API and RAPD-PCR proved to be useful and convenient taxonomic tools for rapid identification at the species level, proving that at least two techniques are needed for precise identification. RAPD-PCR procedure is faster than traditional characterization techniques; the identification of a single colony was typically achieved in less than 10 hr.
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