Generation of two biological wound dressings as a potential delivery system of human adipose derived mesenchymal stem cells (original) (raw)

ASAIO Journal, 2015

Abstract

Human adipose derived mesenchymal stem cells are thought to be potential key factors for starting the regenerative process after tissue injury. However, an efficient method of delivering these regenerative cells to an external wound site is still lacking. Human amnion and pig skin have long been used as skin wound dressings for the treatment of burns and other skin lesions. Here we present the generation of two constructs using these two biomaterials as effective scaffolds for the culture of human adipose derived mesenchymal stem cells(hADMSCs). It was found that hADMSCsseeded onto radio-sterilized human amnion (RHA) and pig skin (RPS) are viable and proliferate. These cells are able to migrate over these scaffoldsas demonstrated by using time-lapse microscopy. Additionally, the scaffolds induce hADMSCs to secrete interleukin-10, an important negative regulator of inflammation; and interleukin-1β, a pro-inflammatory protein.The interplay between these two proteins has been proven to be vital for a balanced restoration of all necessary tissues. Thus, radio-sterilized human amnion and pig skin are likely suitable scaffolds for delivery ofhuman adipose derived mesenchymal stem cellstransplants that could promote tissue regeneration in skin injuries like burned patients.

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