Determination of paternity in dragonflies by Random Amplified Polymorphic DNA fingerprinting (original) (raw)
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This study explores the application of Random Amplified Polymorphic DNA (RAPD) fingerprinting to determine paternity in dragonflies. By utilizing molecular techniques, the research aims to elucidate genetic relationships and improve understanding of mating patterns among these insects. The findings contribute to the field of evolutionary biology and provide insights into the genetic diversity and reproductive behaviors of dragonflies.
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Genetic variation of a migratory dragonfly characterized with random DNA markers
Polymorphism among individuals of dragonflies belonging to same genus and species was studied using molecular technique RAPD-PCR analysis. The RAPD banding pattern reflected the genetic diversity among Pantala flavescens. Reproducible and distinct polymorphic bands ranging approximately 200 bp to 2500 bp were generated with 5 RAPD primers. Operon c series primers OPC 7 and OPC 10 yielded unique bands of 1000 bp, 650 bp and 1100 bp which can be utilized for developing molecular markers for species identification specific for locations. The scoring pattern generated was utilized to construct the distance matrix using POPGENE 32 v1.31.
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