Sex differences in creatine kinase after acute heavy resistance exercise on circulating granulocyte estradiol receptors (original) (raw)

Abstract

Previous research has shown reduced tissue disruption and inflammatory responses in women as compared to men following acute strenuous exercise. While the mechanism of this action is not known, estrogen may reduce the inflammatory response through its interaction with granulocytes. The purpose of this study was to determine if estrogen receptor expression on granulocytes is related to sex diVerences in tissue disruption in response to an acute heavy resistance exercise protocol. Seven healthy, resistance-trained, eumenorrheic women (23 § 3 years, 169 § 9.1 cm, 66.4 § 10.5 kg) and 8 healthy, resistance-trained men (25 § 5 years, 178 § 6.7 cm, 82.3 § 9.33 kg) volunteered to participate in the study. Subjects performed an acute resistance exercise test consisting of six sets of Wve squats at 90% of the subject's one repetition maximum. Blood samples were obtained pre-, mid-, post-, and 1-, 6-, and 24-h postexercise. Blood samples were analyzed for 17-estradiol by ELISA, creatine kinase by colorimetric enzyme immunoassay, and estradiol receptors on circulating granulocytes through Xow cytometry. Men had higher CK concentrations than women at baseline/control. Men had signiWcantly higher CK concentrations at 24-h postex-ercise than women. No signiWcant changes in estradiol receptors were expressed on granulocytes after exercise or between sexes. While sex diVerences occur in CK activity in response to strenuous eccentric exercise, they may not be related to estradiol receptor expression on granulocytes. Thus, although there are sex diVerences in CK expression following acute resistance exercise, the diVerences may not be attributable to estrogen receptor expression on granu-locytes.

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