Separation of acidic proteins by capillary zone electrophoresis and size-exclusion high-performance liquid chromatography: a comparison (original) (raw)

Successful separations of alcohol dehydrogenase (pZ= 5.4), /l-amylase (pZ= 5.2) and albumin (pZ= 4.7) by capillary electrophoresis in uncoated fused-silica capillaries are reported. Different electrophoretic conditions, consisting in variation of temperature, applied voltage and ionic strength of the buffer used as electrolyte, were tested in order to compare the separation efficiency, resolution and selectivity of the acidic proteins. The results were compared with those obtained by size-exclusion chromatography. Rinsing of the capillary between runs, in order to eliminate adsorbed proteins, can shorten its useful lifetime.