Effects of Tepary Bean ( Phaseolus acutifolius ) Protease Inhibitor and Semipure Lectin Fractions on Cancer Cells (original) (raw)
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For many years, several studies have been employing lectin from vegetables in order to prove its toxic effect on various cell lines. In this work, we analyzed the cytotoxic, antiproliferative, and post-incubatory effect of pure tepary bean lectins on four lines of malignant cells: C33-A; MCF-7; SKNSH, and SW480. The tests were carried out employing MTT and 3 [H]-thymidine assays. The results showed that after 24 h of lectin exposure, the cells lines showed a dose-dependent cytotoxic effect, the effect being higher on MCF-7, while C33-A showed the highest resistance. Cell proliferation studies showed that the toxic effect induced by lectins is higher even when lectins are removed, and in fact, the inhibition of proliferation continues after 48 h. Due to the use of two techniques to analyze the cytotoxic and antiproliferative effect, differences were observed in the results, which OPEN ACCESS Molecules 2014, 19 9611
Toxicology in Vitro, 2002
Some protease inhibitors (PI), such as the soybean Bowman-Birk protease inhibitor (SBBI), have been described as anticarcinogenic agents. Although PI are ubiquitous compounds in living organisms, the anticarcinogenic potential of PIs other than SBBI remain poorly explored. We evaluated the antiproliferative effect of a protein fraction from tepary bean (Phaseolus acutifolius) seeds with protease inhibitor activity (TPIF), on normal and on malignant cells. TPIF was obtained after precipitation with ammonium sulfate and gel filtration, and its bioactivity was assayed in vitro on HeLa cells, normal 3T3 fibroblasts and 3T3/v-mos transformed fibroblasts. TPIF showed antiproliferative and cytotoxic effects on 3T3/v-mos transformed fibroblasts in a dose_dependent way. On the contrary, TPIF was only cytostatic for normal 3T3 cells at the highest doses assayed, and had no effect on epithelial HeLa cells proliferation. Sublethal TPIF doses also stimulated cell adhesion of poorly adherent 3T3/v-mos cell line. #
Plant lectins in cancer prevention and treatment
2015
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A Tepary bean lectin fraction (TBLF) has been studied because it exhibits differential cytotoxic and anticancer effects on colon cancer. The present work focuses on the evaluation of the apoptotic mechanism of action on colon cancer cells. Initially, lethal concentrations (LC50) were obtained for the three studied cell lines (HT-29, RKO and SW-480). HT-29 showed the highest LC50, 10 and 100 times higher than that of RKO and SW-480 cells, respectively. Apoptosis was evaluated by flow cytometry, where HT-29 cells showed the highest levels of early and total apoptosis, caspases activity was confirmed and necrosis was discarded. The effect on cell cycle arrest was shown in the G0/G1 phase. Specific apoptosis-related gene expression was determined, where an increase in p53 and a decrease in Bcl-2 were observed. Expression of p53 gene showed the maximum level at 8 h with an important decrease at 12 and 24 h, also the phosphorylated p53(ser46) increased at 8 h. Our results show that TBLF i...
Roles of Plant and Fungal Lectins in Cancer Diagnosis and Treatment: A Scoping Review
DergiPark (Istanbul University), 2022
Article Info Lectins are proteins that possess the carbohydrate-binding property that makes the lectins can bind and recognize carbohydrate moieties on cancer cells. Lectins trigger various cell death forms such as apoptosis, autophagy, and necrosis in various cancer cell lines. These abilities of lectins are making the lectins a potential and convenient tool for cancer treatment and diagnosis. According to carbohydrate specificities and affinities, sequence similarities, the number of carbohydrate-binding domains, etc., lectins are classified into many groups. Therefore, the different lectins in each other have distinct affinities in various cancer cell lines. The researches and reviews of the potential use in cancer treatment and diagnosis of plant and fungal lectins have been aimed to document in this review.
Journal of Food Biochemistry, 2018
Legume comprises approximately 650 genera and 20,000 species including cultivated and wild (Doyle, 1994). Seeds of legume holds substantial amount of protein that possess certain bioactive compounds (Sharon and Lis, 2002). The current knowledge regarding plant proteins with immune-modulatory properties is predominantly based on cultivated species. Studies on wild legumes are desirable and needs to be experimented for their bioactive properties. Lectins are proteins which agglutinate red blood cells with diversity in structure and carbohydrate specificity. Literature perusal suggests putative positive effects of legumes as one of the potential therapeutic candidates on human health. Lectins influence lymphocyte proliferation through cell activity inhibitions vis-à-vis antibody synthesis and cytokine regulation (Nasia, Picariellob, & Ferranti, 2009). Lectins from cultivated species demonstrating various biological activities like mitogenic, antimicrobial and insecticidal activities have been reported (Gautam, Gupta, Narvekar, Bhadkariya, & Bhagyawant, 2018). Data on effects of wild seed lectin are scanty. Published reports entail little information about activities of the wild seed legume showing characteristic features. The example includes Mucuna pruriens seed proteins in treating Parkinson's disease (Shaw & Bera, 1993; Prakash & Tewan, 1999). The seeds of Mucuna birdwoodiana have been employed as curative principles against irregular menstruation and joint pains (Ding, Kinjo, Yang, & Nohara, 1991). Plant lectins have attracted the attention of scientific community because of their remarkable biomedical potential that includes anti-cancerous property as well. Canavalia ensiformis, a wild legume retard the growth of cancer cells (Morris, 1999) while cell proliferation of colon cancer cell lines was repressed by Agaricus bisporus devoid of toxic effects (Parslew,
Cytotoxicity of tuber lectins from two potato cultivars was assessed and their anti-tumor potential against experimentally induced Ehrlich ascites carcinoma in Swiss albino mice was evaluated. Twenty (20) kDa chitin-binding lectins from Solanum tuberosum tubers, STL-S and STL-D were purified through ion-exchange and affinity chromatographic methods, hemagglutinating activity and blood group specificity of the lectins were checked whereas the cytotoxicity was determined using brine shrimp (Artemia salina L.) nauplii lethality assay. The lectins showed no specificity to animal and human erythrocytes. LC 50 values for STL-S and STL-D were found to be 75 and 90 μg/ml, respectively with a dose-dependent intermediary toxic effect. After inducing ascites by intraperitoneal propagation, the Swiss albino mice were treated by administering the lectins at a dose of 1.38 mg/kg/day for five consecutive days. STL-S and STL-D showed 79.84 and 83.04% of growth inhibition of EAC cells, respectively. Additionally, hemoglobin and RBC levels became considerably increased with a drop off in the WBC levels in the treated mice group indicating moderate anticancer activities exhibited by the potato lectins.