Unique communities of anoxygenic phototrophic bacteria in saline lakes of Salar de Atacama (Chile): evidence for a new phylogenetic lineage of phototrophic Gammaproteobacteriafrom pufLMgene analyses (original) (raw)

An oval to rod-shaped, phototrophic, purple non-sulfur bacterium, strain JA192 T , was isolated from an enrichment culture of a pasteurized rhizosphere soil sample from a field cultivated with jowar (sorghum) collected from Godumakunta village near Hyderabad, India. Strain JA192 T is Gram-negative, motile and produces endospores. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that the strain JA192 T is closely related to Rhodobacter sphaeroides 2.4.1 T (99.9 % sequence similarity), Rba. megalophilus JA194 T (99.8 %) and Rba. azotoformans KA25 T (98.1 %) and clusters with other species of the genus Rhodobacter of the family Rhodobacteraceae. However, DNA-DNA hybridization with Rba. sphaeroides DSM 158 T , Rba. megalophilus JA194 T and Rba. azotoformans JCM 9340 T showed relatedness of only 38-57 % with respect to strain JA192 T . On the basis of 16S rRNA gene sequence analysis, DNA-DNA hybridization data and morphological, physiological and chemotaxonomic characters, strain JA192 T represents a novel species of the genus Rhodobacter, for which the name Rhodobacter johrii sp. nov. is proposed. The type strain is JA192 T (5DSM 18678 T 5JCM 14543 T 5MTCC 8172 T ).