Hardion et al. 2013. IAPT/IOPB Chromosome data 15, edited by K. Marhold (original) (raw)
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Paspalum is one of the most important genera of the Poaceae family due to its large number of species and diversity. The subgenus Anachyris comprises six species mainly from South America grouped together by sharing rare spikelet characteristics. A genetic analysis using ISSR markers, compared with the morphological and phenotypic variation observed in each one species, was used to establish genetic relationships among 40 accessions with several ploidy levels, belonging to 5 species of the subgenus Anachyris. Fourteen accessions of Paspalum malacophyllum (2x and 4x), 12 of P. simplex (2x, 3x, 4x and 6x), 4 of P. procurrens (2x and 4x), 4 of P. usterii (4x) and 6 of P. volcanensis (4x) were analysed. A total of 227 ISSR loci (98.7% polymorphic) were detected among all accessions, with variable loci number and percentages of polymorphism according to species delimitations. Six main groups were identified by cluster analysis based on Jaccard's genetic distance and UPGMA, four of which matched all the respective accessions of P. simplex, P. procurrens, P. usterii and P. volcanensis, while the other two were consistent with two different groups of accessions of P. malacophyllum, one involving most tetraploid accessions, and the other one grouping together a tetraploid and two diploid accessions. The distinctive morphological characteristics and the separate clustering of these tetraploid and diploid cytotypes suggest to consider a new multiploid species complex inside the subgenus Anachyris. Both cytotypes of P. procurrens, and the four co-specific cytotypes of P. simplex consistently clustered together forming two specific groups for the two multiploid taxons. This is in agreement with the existence of high phenotypic similarities between diploid and tetraploid cytotypes of P. procurrens, and among diploid, triploid, tetraploid and hexaploid cytotypes of P. simplex. Since the polyploid cytotypes of these species are reproduced by apomixis, the specific genetic clustering by ISSR markers and morphological and cytological results support the hypothesis that the two multiploid species were originated by autopolyploidy. Our results confirm previous studies suggesting a monophyletic origin for the subgenus Anachyris and are concordant with previous data regarding genomic homologies and phylogenetic analyses in the genus.
Stellaria. In. MARHOLD, K., (ed.), IAPT/IOPB chromosome data 11
Taxon
Internal standard (Petunia hybrida PxPC6, 2C = 2.85 pg) was used to determine DNA content by flow cytometry (Partec CyFlow 532 nm laser cytometer). This study was supported by the town community of Fréjus SaintRaphaël and the Société Botanique de France. Authors are grateful to colleagues that helped us in plant localisation and sampling.
Bouchal_et_al_2018_Supplementary_MAterial_S2_genus level climscore.xlsx
Pollen morphology and taxonomy of Eurasiatic species of the genus Buxus (Buxaceae). Grana, 31, 65-78. Caudullo G, Welk E, San-Miguel-Ayanz J (2017) Chorological maps for the main European woody species. Data in Brief, 12, 662-666. Christenhusz MJM (2013) Twins are not alone: a recircumscription of Linnaea (Caprifoliaceae). Phytotaxa, 125, 25-32. Clement WL, Donoghue MJ (2012) Barcoding success as a function of phylogenetic relatedness in Viburnum, a clade of woody angiosperms.
International Journal of Plant Sciences, 2015
Premise of research. Riparian plants are highly dependent on water sources; consequently, general climatic conditions are less important to these taxa relative to woodland and shrubland species. This leads to interesting research questions regarding riparian plant taxa. Research on phylogeography of Mediterranean riparian tree and shrub species is scarce. In this article, we investigated the plastidial genetic diversity in Celtis australis L. (hackberry) and Nerium oleander L. (oleander) throughout the Mediterranean Basin. Both species are distributed in gullies, rivers, and stream banks under warm temperate climates. Methodology. Eighteen cpSSR loci and three noncoding cpDNA regions (rps16, rpl32-trnL, and trnQ-5′-rps16) were examined to assess the levels and geographic distribution of sequence variation in 41 hackberry and 56 oleander populations. The rpl32-trnL intergenic region was used for molecular dating analysis. Pivotal results. The respective 2762-and 3134-bp noncoding cpDNA regions sequenced in C. australis and N. oleander, as well as the 22 cpSSR analyzed fragments, exhibited the absence of variability in natural populations throughout the Mediterranean Basin. In N. oleander, two regions (rps16, rpl32-trnL) exhibited variability in three positions, and four cpSSR microsatellite motifs were polymorphic. The polymorphisms were geographically structured, and three haplotypes were characterized, two from Saharan populations and one from Mediterranean populations. Phylogeny and molecular dating analyses resulted in a tree with high consistency values of posteriori probability (PP p 1) and bootstrap support (98%) from the ingroup (N. oleander). The relaxed molecular clock model applied to the calibration of the tree estimates that the diversification of these haplotypes occurred in a range of 7.2-1.2 Ma. Conclusions. The absence of plastid variability in both hackberry and oleander is explained by a low mutation rate and/or recent recolonization of the Mediterranean Basin. Low temperatures during the Last Glacial Maximum produced freezing water along riparian corridors, with the consequent drastic contraction or even disappearance of both species in the Mediterranean Basin.
Biologia, 2008
Using flow cytometry in fresh plants and herbarium vouchers, DNA ploidy levels for 411 individuals of 44 taxa of the genus Festuca, including 4 natural hybrids, originating from 237 sites in Austria, Bulgaria, Croatia, Czech Republic, Estonia, Germany, Hungary, Italy, Poland, Romania, Slovakia, Slovenia, and Switzerland were estimated. The following taxa and DNA ploidy levels are reported: F. airoides (2n ≈ 2x), F. alpestris (2n ≈ 2x), F. alpina s.l. (2n ≈ 2x), F. amethystina subsp. amethystina (2n ≈ 4x), F. bosniaca subsp. bosniaca (2n ≈ 2x), F. brevipila (2n ≈ 6x), F. bucegiensis (2n ≈ 2x), F. carnuntina (2n ≈ 6x), F. csikhegyensis (2n ≈ 4x), F. csikhegyensis × F. eggleri (2n ≈ 4x), F. dalmatica (2n ≈ 4x), F. duvalii (2n ≈ 4x), F. eggleri (2n ≈ 2x, 4x), F. filiformis (2n ≈ 2x), F. glauca (2n ≈ 6x), F. heterophylla (2n ≈ 4x), F. inops (2n ≈ 2x), F. laevigata (2n ≈ 8x), F. laxa (2n ≈ 4x), F. lemanii (2n ≈ 6x), F. norica (2n ≈ 2x), F. ovina subsp. ovina (2n ≈ 2x), F. ovina subsp. gue...
Taxon, 2007
All materials CHN, collected in France by M. Espeut and A. Aboucaya, counted by R. Verlaque; vouchers in MARS. VIOLACEAE Viola arborescens L., 2n = 52; Aboucaya 14, 15, 16, 17, 18 Viola arvensis Murray, 2n = 34; Espeut 42, 51 2n = 36; Espeut 50 Viola bubanii Timb.-Lagr., 2n = 42-44; Espeut 46 2n = 52; Espeut 49 2n = 120; Espeut 52 Viola canina L., 2n = 40; Espeut 44 Viola kitaibeliana Schult., 2n = 16; Espeut 2, 41, 37, 39 2n = 48; Espeut 38 Viola pseudomirabilis Coste, 2n = 40; Espeut 31 Viola pyrenaica DC., 2n = 20; Espeut 35 Viola reichenbachiana Boreau, 2n = 20; Espeut 32 Viola roccabrunensis Espeut, 2n = 48; Espeut 24, 33 Viola suavis M. Bieb., 2n = 40; Espeut 34, 28 Viola tricolor L. subsp. tricolor, 2n = 26; Espeut 45 Viola × wittrockiana Gams, 2n = 37-38; Espeut 43