Alveolar bone loss associated to periodontal disease in lead intoxicated rats under environmental hypoxia (original) (raw)
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The influence of lead on gingiva and periodontal tissue
Stomatološki glasnik Srbije, 2023
Introduction Exposure to lead (Pb) can have harmful effects on the general state of the body, including oral health. The aim of this study was to examine the prevalence of changes in gingiva and periodontal tissue in adult rats intoxicated with lead acetate by histological analysis. Material and methods The study was conducted on 42 Wistar rats. Intoxication of rats with lead acetate was carried out through drinking water ad libitum. The first group (A1) consisted of 16 rats that received lead-acetate in water at a concentration (1500 ppm) for 14 days, and the second group (A2) consisted of 16 rats that received lead-acetate in water at the same concentration for 30 days. Results Gingivitis and periodontitis in the first group of rats (A1) were observed in 41.7% of cases, and in the second group (A2) in 84% of cases. A statistically significant difference was found between the investigated groups (χ 2 = 6.955; p < 0.018). Conclusion Since changes in the gingiva and periodontium have been observed in rats intoxicated with lead for a long period of time, it is necessary to propose a preventive program for patients exposed to lead, which includes dental procedures such as oral hygiene training and regular visits to the dentist.
Lead intoxication under environmental hypoxia impairs oral health
Journal of toxicology and environmental health. Part A, 2014
We have reported that chronic lead intoxication under hypoxic environment induces alveolar bone loss that can lead to periodontal damage with the subsequent loss of teeth. The aim of the present study was to assess the modification of oral inflammatory parameters involved in the pathogenesis of periodontitis in the same experimental model. In gingival tissue, hypoxia increased inducible nitric oxid synthase (iNOS) activity (p < .01) and meanwhile lead decreased prostaglandin E2 (PGE2) content (p…
The effect of airborne lead on lead levels of blood, incisors and alveolar bone of rats
Archives of Oral Biology, 1991
~roups of 20 inbred rats were exposed to: (1) "clean air" (0.05 pg Pb/m3) for 70 days; (2) 77 pg Pb/m' for 70 days; (3) 249 pg Pb/m' for 28 days; and (4) 1546 pg Pb/m3 for 50 days. Half the rats in each group were then killed and the other half kept in "clean air" until the blood lead of groups 1-3 had returned to normal. That half of the incisors which had been completely renewed during the experimental period, as well as the alveolar bone of each rat, were analysed for lead by atomic absorption spectrophotometry. In the rats killed immediately after exposure, the lead levels of the incisors and alveolar bone showed significant differences (p < 0.05) among all four groups, except between groups 2 and 3, indicating the importance of the combined effect of airborne lead concentration and length of exposure. For blood, significant differences (p c 0.05) were found among all groups. For the postexposure rats, the incisors and blood showed no significant differences (p > 0.05) among groups I,2 and 3. For alveolar bone, significant differences were not found (p > 0.05) between groups 2 and 3. It can be concluded that during lead exposure the alveolar bone transformation was the highest and that of incisor tissue just lower, and that blood lead became supplemented through the process of remobilization from alveolar bone, as reported for other bones.
Lead Bone Toxicity in Growing Rats Exposed to Chronic Intermittent Hypoxia
Bulletin of Environmental Contamination and Toxicology, 2012
Lead chronic intoxication under hypoxic conditions revealed growth retardation in growing rats and damages on femoral and mandibular bones that predispose to fractures. These findings aimed us to investigate if bone material and geometric properties, bone mass in terms of histomorphometry or antioxidant capacity are also impaired in such experimental model. Combined treatments significantly reduced hemimandible cross sectional geometry and intrinsic stiffness (-16 % and-34 %); tibia and hemimandible bone volume (-45 % and-40 %) and growth plate cartilage thickness (-19 %). These results show a previously unreported toxic effect of lead on mandible however, longer studies should be necessary to evaluate if an adaptation of bone architecture to maintain structural properties may occur and if the oxidative stress can be identified as the primary contributory agent in the pathogenesis of lead poisoning.
Determination of systemically & locally induced periodontal defects in rats
The Indian journal of medical research, 2005
The role of lathyrogens on bone metabolism is unclear, therefore we undertook this study to observe periodontal and systemic alterations in experimental lathyrism in rat and compare these changes to that observed in the locally induced periodontitis group. A total of 45 male Wistar rats were equally divided in the lathyritic group (group 1), ligature-induced periodontitis group (group 2), and healthy controls (group 3). Experimental lathyrism was induced by once daily subcutaneous administration of beta-aminoproprionitrile (beta-APN), at a dose of 5 mg/0.4 ml per 100 g of body weight for 40 days. Ligature-induced periodontitis was created by tying silk ligatures on the necks of mandibular molars. After 40 days, blood samples were obtained and the animals were decapitated. Radiographic observations, extraction tests, histologic evaluations were performed, and serum ALP activity and gingival tissue IL-1beta levels were measured. Significant alveolar bone resorption around the mandibul...
2000
O presente estudo teve por objetivo avaliar radiograficamente, os efeitos provenientes da adaptação da ligadura durante a indução da doença periodontal em ratos. Para este estudo foram usados 30 ratos. No primeiro molar inferior foi adaptada uma ligadura a qual foi distribuída de forma randômica, entre o lado direito e esquerdo. O lado sem ligadura foi utilizado como controle (negativo). Logo após a adaptação da ligadura, esta foi removida. Os animais foram sacrificados no período de 0, 3 e 7 dias, tendo dez animais por período. Desta forma as mandíbulas foram removidas, separadas e fixadas em formol. As hemimandíbulas foram radiografadas e analisadas pelo sistema de imagem digital Digora, onde à distância da união cemento-esmalte à crista óssea alveolar foi mensurada em mm por um traçado linear. Os dados coletados não indicaram diferença estatisticamente significante entre os grupos e períodos experimentais analisados pelo teste T de student (p>0,05). Dentro dos limites deste estudo e da metodologia utilizada podemos concluir que a ligadura não traumatiza os tecidos periodontais e por si só não leva ao desenvolvimento da doença periodontal em ratos.
Blood Oxidative Stress Modulates Alveolar Bone Loss in Chronically Stressed Rats
International Journal of Molecular Sciences, 2020
We aimed to investigate the effects of chronic stress (CS) on experimental periodontitis (EP) in rats. For this, 28 Wistar rats were divided into four groups: control, ligature-induced experimental periodontitis (EP), chronic stress (CS; by physical restraint model) and CS+EP (association of chronic stress and ligature-induced periodontitis). The experimental period lasted 30 days, including exposure to CS every day and ligature was performed on the 15th experimental day. After 30 days, the animals were submitted to the behavioral test of the elevated plus maze (EPM). Next, rats were euthanized for blood and mandible collection in order to evaluate the oxidative biochemistry (by nitric oxide (NO), reduced-glutathione activity (GSH), and thiobarbituric acid reactive substance levels (TBARS)) and alveolar bone characterization (by morphometric, micro-CT, and immunohistochemistry), respectively. The behavioral parameters evaluated in EPM indicated higher anxiogenic activity in the CS a...