Effect of growth rate on stability and gene expression of a recombinant plasmid during continuous culture ofEscherichia coli in a non-selective medium (original) (raw)

Experimental results were obtained with Escherichia coli C600 galK(GAPDH), a genetically engineered strain that synthetizes a large quantity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), (80 % of the soluble proteins). Data concerning the stability of plasmid-containing cells and gene expression as a function of dilution rate have been obtained in continuous cultures. Contrary to other studies, our results show a clear indication that the rate of the recombinant activity was dependent on dilution rate. The results support the finding that the apparent stability of the plasmid decreases with dilution rate.