Isolation and identification of a flavone (quercetin) from Butea frondosa bark (original) (raw)
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2020
In this research work, the stem bark of ButeafrondosaRoxb., Myanmar name Pauk, was selected for chemical analysis. This samplewas collected from Amarapura Township, Mandalay Region, Myanmar. The aim of this research is to get more interesting and bioactive compounds from this versatile medicinal plant. Firstly, the preliminary phytochemical test of this selected plant was carried out. Moreover, the total phenols of selected sample were extracted with methanol and distilled water. This extract was checked for qualitative test of phenols. In addition, total phenolic content of selected sample was evaluated by the FolinCiocalteau reagent using UV spectrophotometer at 765 nm. The total phenolic content ofselected sample was found to be15.40.023 mg gallic acid equivalents (GAE) per g dry weight.Furthermore, the potent pure compound was isolated by Column Chromatography and HPLC methods atMeijo University, Nagoya, Japan. The structure of isolated compound was elucidated as flavonoid by a...
The present work attempts to evaluate the physicochemical and preliminary phytochemical studies on the leaves of Butea frondosa Koen. Ex Roxb, family Fabaceae. Methods: The herbal standardization was carried out on the basis of organoleptic properties, physical characteristics, and physico-chemical properties. Physicochemical parameters including ash values, extractive values, loss on drying, foreign matter were evaluated. Results: Macroscopically leaves were observed to be compound. Lateral leaflets were obliquely ovate, obtuse, round at apex. Its texture was fairly tough. Microscopical leaves have single layered upper and lower epidermis, covered with thick cuticle. Xylem and phloem are arranged in ring. Calcium oxylate crystal were scattered throughout the cells. The total ash, acid insoluble ash and water soluble ash were found to be 10.16%, 2.83%, and 5.16% w/w respectively. Petroleum ether, Chloroform, ethanol and water soluble extractive values (hot)were 2.94%, 3.08%, 5.06%, 10.61%w/w respectively. The pH of 1% and 10% aqueous solution was found to be 6.06 and 5.76 respectively. Preliminary phytochemical screening showed the presence of sterols, tannins, flavonoids, amino acids, glycosides, phenolic compounds, carbohydrates, saponins and alkaloids. Thin layer chromatographic studies also had been done on ethanolic and aqueous extracts. Conclusion: These studies provided referential information for correct identification and standardization of this plant material.
Two new phytosterols from the stem bark of Ficus bengalensis L
Phytochemical investigation of the methanolic extract of the stem bark of Ficus bengalensis L. (Moraceae) resulted in the isolation of two new phytoconstituents characterized as lanost-5,24-dien-3b-yl-b-D-glucopyranosid-2 0-O-yl-docos-11 00-enoate (lanostadienylglucosyl cetoleate) (1) and 3b-acetoxy-stigmast-22-en-26-oic acid (bengalensisteroic acid ester) (2) together with the known compounds heneicosanyl cis-octadec-9-enoate (heneicosanyl oleate) (3), urs-12-en-3b-yl acetate (a-amyrin acetate) (4), and lup-20(29)-en-3b-ol (lupeol) (5). The structures of these phytoconstituents have been established on the basis of spectral data analysis, chemical reactions and comparison of literature. ª 2012 Production and hosting by Elsevier B.V. on behalf of King Saud University.
Ethnomedicinal, Phytochemical and Pharmacological Aspects of Flacourtia jangomas: A Review
International Journal of Pharmacy and Pharmaceutical Sciences 10(3):9-15, 2018, 2018
Flacourtia jangomas (Lour.) Raeusch., a small tree of Willow family distributed throughout tropical regions of East Africa and Asia is well-known for its culinary and medicinal uses. The plant has received increasing interest due to its limonoid constituents. Phytochemical studies have led to the isolation and characterization of an array of bioactive compounds and pharmacological investigations have validated the traditional uses of the species as well as discovered some new bioefficacies. The aim of the review is to present an updated and comprehensive overview of traditional uses, ethnomedicinal significance, phytochemical and pharmacological aspects of F. jangomas to highlight its ethnopharmacological use and to explore its therapeutic potentials thereby providing a basis for future research. Relevant information and literature on F. jangomas from electronic databases such as Academic Journals, Google, Google Scholar, PubMed, Web of Science, and Science Direct were consulted and analyzed. Available literatures evidently demonstrate that F. jangomas possess a wide spectrum of pharmacological activities that could be explained by the presence of varied range of chemical constituents. Phytochemical and pharmacological investigations showed that extracts of different parts and major active components of F. jangomas had antimicrobial, analgesic, antidiabetic, antidiarrheal, antioxidant and cytotoxic activities. This emphasizes the need for further investigation to explore more bioactive chemical constituents and new bioefficacies of the plant and to establish a credible relationship between molecular structure and activity of compounds.
Determination of Some Flavonoids and Antimicrobial Behaviour of Some Plants' Extracts
Indian Journal of Pharmaceutical Education and Research
C. sativa, C. intybus, L. stoechas, V. officinalis and G. glabra plants were extracted by using 65% ethanol to isolate their active constituents. The antimicrobial activities of extracts were investigated against 15 microorganisms by using the disk diffusion method, MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration) and MFC (Minimal Fungicidal Concentration) tests. Furthermore, the presence of eight flavonoids were analysed by using HPLC. It was found that C. sativa is active against C. albicans, E. faecalis, S. enteritidis and S. typhimurium with MIC values of 26.02 µg/mL, 13.01 µg/mL, 416.25 µg/mL and 832.50 µg/mL respectively, where C. intybus is active against C. albicans and E. faecalis, with MIC values of 13.01 µg/mL and 6.50 µg/mL, respectively. On the other hand, L. stoechas and V. officinalis were observed to be active against only S. enteritidis with MIC values of 52.03 µg/mL and 26.02 µg/L respectively, where G. glabra was active against only E. faecalis, with a MIC value of 52.03 µg/mL. The extracts of plant samples showed antibacterial activity against tested microorganisms at different levels. But the activities against C. albicans and E. faecalis is noteworthy. The flavonoids were determined at different amounts in extracts.
Determination Of The Presence Of Flavonoids In The Leaves, Seeds And Branches Of The Matured
2015
Abstract: The extracts were concentrated to obtain a mass of 0.9g, 1.2g, 1.4g of seeds, leaves and branches respectively. After the separation of the pigment into hexane, the mass of the residue obtained were 0.7g for seeds, 0.9g for leaves and 1.0g for branches. The Cyanidin’s test for the various masses of the extracts of the plant materials after washing with hexane gave the evidence of the presence of flavonoids. The number of components could not be obtained from the eluates of the column chromatography, because there were no T.L.C materials available. But when the component of each plant material was subjected to cyanidin’s test, it gave the proof of the presence of flavonoid.
Flavonoids and other bioactive constituents from Ficus thonningii Blume (Moraceae)
Phytochemistry Letters, 2015
Continued interest in the chemistry of Ficus spp. led to the investigation of the figs and the roots of Ficus thonningii Blume. Two new flavonoids, thonningiol (1) and thonningiisoflavone (2) along with nineteen known compounds were isolated. b-Isoluteone (13) was isolated here for the first time from a natural source. Their structures were elucidated on the basis of spectroscopic evidence. Interestingly, thonningiisoflavone (2) and hydroxyalpinumisoflavone showed strong DPPH radical scavenging activity with IC 50 = 65.50 mM and 68.20 mM respectively compared to the standard BHA with IC 50 = 44.20 mM. The methanolic extract of figs, taxifolin (14), conrauiflavonol (17) and shuterin exhibited moderate antimicrobial activity against six micro-organisms with MIC below 1.5 mg/mL.