Coordinated binding of Vps4 to ESCRT-III drives membrane neck constriction during MVB vesicle formation (original) (raw)

The study investigates the role of Vps4 in the formation of intraluminal vesicles (ILVs) during multivesicular body (MVB) biogenesis, focusing on how Vps4 coordinates with ESCRT-III to facilitate membrane neck constriction, which is crucial for ILV scission. Through a combination of yeast genetics, biochemistry, and electron tomography, the authors demonstrate that while ESCRT-III assembly on endosomes is vital for neck stabilization, the functional collaboration of Vps4 is essential to complete the membrane-remodeling processes that lead to effective ILV formation.