Postsurgical Administration of Estradiol Benzoate Decreases Tensile Strength of Healing Skin Wounds in Ovariectomized Rats (original) (raw)
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Journal of Hormones, 2014
The aim of this experiment was to investigate the effect of 17β-estradiol on cutaneous wound healing in 12-week ovariectomized (OVX) female mice. Eight-week-old female mice were divided into three groups: administration of 17β-estradiol after OVX (OVX + 17β-estradiol), OVX, and sham (SHAM). Four weeks after surgery, the mice received two full-thickness cutaneous wounds. 17β-Estradiol at 0.01 g/day was administered on the backs of mice in the OVX + 17β-estradiol group every day. Plasma 17β-estradiol level in the OVX + 17β-estradiol group was thus significantly higher than in the SHAM and OVX groups, but there was no significant difference between SHAM and OVX groups. The ratio of wound area was not significantly different among the three groups. However, the period required to reach a ratio of wound area of 0.15 in the OVX + 17β-estradiol group was significantly shorter than in the SHAM and OVX groups. These results indicate that cutaneous wound healing in young OVX mice was promoted...
Effects of ovariectomy and hormone replacement on collagen and blood vessels 2003
Collagen and blood vessels of the urethral submucosa of ovariectomized rats were studied following 28 daily subcutaneous injections of 17-ß estradiol (n=6, group 1), medroxy-progesterone acetate (n=6, group 2), both drugs (n=6, group 3) or vehicle (n=6, control) and after sham surgery without castration or injection (n=6). Investigations included the immunohistochemistry of estrogen and progesterone receptors and collagen fibres, Western blot analysis of collagen types I and III and counting periurethral vessels by light microscopy. Our results showed positive immunostaining with estrogen, progesterone and collagen types I and III in all samples. Collagen type I and III levels were lower in the controls than in the sham group. The other groups showed increases (2>3>1) over the controls with a relatively higher increase in type III. The type I/III collagen ratio progressively decreased (con-trol>1>2>3) below sham levels. The mean vessel count was significantly lower in control than in sham animals (P<0.00001). However, only estrogen treatment significantly increased the vessel number compared to controls (P=0.04). Our results indicate that estrogen and progesterone, alone or in combination, have an effect on collagen types I and III, and that estrogen has an effect on blood vessels of the urethral submucosa in female rats.
Journal of Baghdad College of Dentistry, 2015
Background: Wound healing, as a normal biological process in the human body, is achieved through four precisely and highly programmed phases: hemostasis, inflammation, proliferation, and remodeling. Growth factors released in the traumatized area promote cell migration into the wound area (chemotaxis), stimulate the growth of epithelial cells and fibroblasts (mitogenesis), initiate the formulation of new blood vessels (angiogenesis), and stimulate matrix formation and remodeling of the affected region. One of factors that effects on wound healing is a sex hormones and one of these hormones is an estrogen hormone. A wide range of cutaneous cell types (eg, fibroblast, endothelial, epithelial, and inflammatory) expressed estrogen receptors, indicating potential estrogen responsiveness. Materials and methods: Thirty two female New Zealand rabbits were used in this study. All animals were ovariectomized, and incisional wounds were done on the right (experimental for estrogen hormone application) and left (control) sides of face for each animal, the control side was left to heal normally. Histological assessment regarding the count of inflammatory cells was performed for healing intervals (3, 7, 10, 14 days). Results: Topical estrogen hormone application revealed enhancement of wound healing by reducing wound size and stimulating matrix deposition in comparison to control. Conclusion: Topical estrogen cream application results in significant progress of cutaneous wound healing, leaving no scar or crust formation and can minimize the probable wound complications. Key words: Estrogen hormone, wound healing.
PLOS ONE, 2016
Estrogen promotes cutaneous wound healing in ovariectomized (OVX) female mice. However, the effects of topical estrogen application on wounds remain unclear. Therefore, the aim of this study was to compare the effects of topical estrogen application on wounds with standard treatment methods. Eight-week-old C57BL/6J female mice underwent OVX and received two full-thickness wounds four weeks later. Mice were divided into three groups: topical estradiol benzoate (EB) (0.75 μg/g/day) wound treatment, subcutaneous estradiol (E2) pellets (0.05 mg, 21 days), and topical E2 (0.01 g/day) skin application. Wound healing was observed until day 14. Wound area ratios were significantly smaller in the topical EB wound treatment group than in the subcutaneous E2 pellet group on days 1-14 (p < 0.05) and topical E2 skin application group on days 1-9 (p < 0.05). Neutrophil and macrophage numbers were significantly smaller in the topical EB wound treatment group than in the subcutaneous E2 pellet and topical E2 skin application groups on day 7 (p < 0.05). Moreover, the number of new blood vessels and ratio of myofibroblasts were significantly larger in the topical EB wound treatment group than in the subcutaneous E2 pellet and topical E2 application skin groups on day 7 (p < 0.05). These results demonstrate that the application of estrogen to wounds reduced inflammatory responses and promoted angiogenesis and wound contraction more than the two other standard treatment methods.
Journal of Hormones, 2014
Estrogen replacement promotes cutaneous wound healing in 8-10-week young ovariectomized female mice. However, research using aged ovariectomized female mice has not been reported, to the best of our knowledge. Therefore, we investigated the effect of 17 -estradiol on cutaneous wound healing using 24-week middle-aged ovariectomized female mice. Twenty-week-old female mice were divided into three groups: medication with 17 -estradiol after ovariectomy (OVX + 17 -estradiol), ovariectomy (OVX), and sham (SHAM). After 4 weeks, the mice received two full-thickness wounds. Then, the OVX + 17 -estradiol group was administered 17 -estradiol at 0.01 g/day until healing. The ratio of wound area in the OVX + 17 -estradiol group was significantly decreased compared with that in the OVX group. The numbers of neutrophils and macrophages in the OVX + 17 -estradiol group were significantly smaller than those in the OVX group. In addition, the ratio of myofibroblasts in the OVX + 17 -estradiol group was significantly higher than that in the OVX group. These data suggested that exogenous continuous 17 -estradiol administration promotes cutaneous wound healing in 24-week OVX female mice by reducing wound area, shortening inflammatory response, and promoting wound contraction. However, it is unclear whether the effect of exogenous estrogen on wound healing outweighs the delay of wound healing due to advanced age.
Investigation of biological and wound healing effects of Estrogen solution: An in vitro study
Background Repairing dermal skin defects denotes a challenging obstacle in wound healing. Wound healing activities of estrogen have been noted in many experimental models proposing their beneficial role in wound closure and treatments of impaired wound healing. To study the most significant problem in dermal defect regeneration, namely collagen formation and insufficient blood supply, this study aimed to evaluate different concentrations of estrogen in the co-culture of fibroblast and endothelial cells. Methods The human fibroblast (C163) and Human umbilical vein endothelial cells (HUVEC) were co-cultured and treated with different concentrations of estrogen solution. The cytotoxic effect of estrogen solution was evaluated by MTT assay while expression of endothelial markers (CD31) and Vimentin in treated cells was examined using Real-time PCR and Immunofluorescence analysis. Wound healing capacity in human fibroblast cells was studied by a scratch test assay. Results Estrogen has a...
PLoS ONE, 2014
Cutaneous wound healing is delayed by protein malnutrition (PM). On the other hand, estrogen promotes cutaneous wound healing by its anti-inflammatory and cell proliferation effects. Therefore, we hypothesized that estrogen administration in protein-malnourished ovariectomized (OVX) female mice might improve the inflammatory response and promote cutaneous wound healing as well as normal nutrition. To test this hypothesis, we used full-thickness excisional wounds in Control SHAM, PM SHAM, PM OVX and PM OVX+17b-estradiol mice. The Control diet included 200 g/kg protein and the PM diet included 30 g/kg protein. The ratio of wound area in the Control SHAM group was significantly smaller than those in the three PM groups. In addition, microscopic findings also showed that the ratio of collagen fibers, the ratio of myofibroblasts and the number of new blood vessels in the Control SHAM group were significantly greater than those in the three PM groups. However, the number of Ym1-positive cells as an anti-inflammatory M2-like macrophage marker in the PM OVX+17b-estradiol group was significantly higher than those in the other three groups. These results indicate that the appearance of anti-inflammatory M2-like macrophages was promoted by estrogen administration; however, it could not promote cutaneous wound healing upon a low-protein diet. Therefore, it may be confirmed that nutrition is more important for promoting cutaneous wound healing than estrogen administration.
Urological …, 2003
Collagen and blood vessels of the urethral submucosa of ovariectomized rats were studied following 28 daily subcutaneous injections of 17-ß estradiol (n=6, group 1), medroxy-progesterone acetate (n=6, group 2), both drugs (n=6, group 3) or vehicle (n=6, control) and after sham surgery without castration or injection (n=6). Investigations included the immunohistochemistry of estrogen and progesterone receptors and collagen fibres, Western blot analysis of collagen types I and III and counting periurethral vessels by light microscopy. Our results showed positive immunostaining with estrogen, progesterone and collagen types I and III in all samples. Collagen type I and III levels were lower in the controls than in the sham group. The other groups showed increases (2>3>1) over the controls with a relatively higher increase in type III. The type I/III collagen ratio progressively decreased (con-trol>1>2>3) below sham levels. The mean vessel count was significantly lower in control than in sham animals (P<0.00001). However, only estrogen treatment significantly increased the vessel number compared to controls (P=0.04). Our results indicate that estrogen and progesterone, alone or in combination, have an effect on collagen types I and III, and that estrogen has an effect on blood vessels of the urethral submucosa in female rats.