Influence of both media carbon source and strains variety on the Biofilms formation ability by Pseudomonas aeruginosa (original) (raw)
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Archives of Biological Sciences, 2014
In the present study, we have examined if there is any difference in biofilm production among different genotypes of Pseudomonas aeruginosa. The study investigated 526 non-duplicate P. aeruginosa isolated from clinical specimens and from a hospital environment. Isolates were grouped into thirty-five genotypes based on an identical ERIC2-band pattern. Biofilm formation was quantified by the microtiter plate test and all strains were classified into the following categories: no biofilm producers (0), weak (+), moderate (+), or strong (+++) biofilm producers. Only 2.45% of examined strains were not biofilm producers. Among biofilm producers, 39.26% were strong biofilm producers, 34.36% were moderate biofilm producers, while 23.93% were weak biofilm producers. Although the majority of strong biofilm producers were in genotype groups 2 and 3, the degree of in vitro biofilm formation in our study was not significantly affected by the genotype of Pseudomonas aeruginosa. In this study, we demonstrated that the degree of in vitro biofilm formation is not significantly affected by the genotype of Pseudomonas aeruginosa.
Advances in Microbiology, 2020
Biofilms are dense bacterial colonies, derived from microbially derived sessile community, networked within a polysaccharide matrix with a distinct architecture that has the attachment potential to both alive and abiotic surfaces. Pseudomonas aeruginosa is a model biofilm forming microorganism associated with remarkable morbidity and mortality rate due to emergence of antibiotic resistant pathogenic bacteria. Moreover, Pseudomonas aeruginosa originating from a biofilm is more resistant to a wide range of antibiotics than the planktonic bacteria. This research was planned to develop a comparative study of the biofilm production between potential, antimicrobial resistance of Pseudomonas aeruginosa isolated from mature environmental biofilm and clinical strain of the same species that did not derive from biofilm. It was observed that the Pseudomonas aeruginosa from environmental isolates were resistant to 15 prominent antibiotics, while clinical strain was comparatively resistant to only few of them. A confirmatory analysis of biofilm formation and antibiotic resistance pattern of these two groups of organisms was checked by 96-well microtiter plate and the disc diffusion method respectively. Finally, the results portrayed that the environmental strains with high drug resistance, potentially formed a considerable amount of biofilm in the period of a week whereas; clinical stains formed a negligible amount of biofilm within the same time frame.
Biofouling, 2014
The relationship between the environmental conditions of biofilm formation and resistance to disinfectants was studied. Anti-biofilm assays were performed against biofilms grown at 20, 30 and 37°C on stainless steel and polycarbonate, over 24 and 48 h. A rise in growth temperature increased the resistance of 24 h biofilms to disinfectants containing didecyldimethylammonium chloride and decreased it to a disinfectant containing alkyldimethylbenzylammonium chloride. The increase in growth temperature coupled with an incubation time of 24 h promoted increases in both matrix production and the membrane rigidity of sessile cells. An increase in incubation time also increased both matrix production and the membrane rigidity of sessile cells. Such phenomena resulted in an increased resistance to disinfectants of biofilms grown at 20 and 30°C. The resistance of 48 h biofilms to disinfectants decreased with an increase in growth temperature despite the increase in matrix production and the membrane rigidity of sessile cells.
Pseudomonas aeruginosa Biofilm Formation and Its Control
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Microbes are hardly seen as planktonic species and are most commonly found as biofilm communities in cases of chronic infections. Biofilms are regarded as a biological condition, where a large group of microorganisms gets adhered to a biotic or abiotic surface. In this context, Pseudomonas aeruginosa, a Gram-negative nosocomial pathogen is the main causative organism responsible for life-threatening and persistent infections in individuals affected with cystic fibrosis and other lung ailments. The bacteria can form a strong biofilm structure when it adheres to a surface suitable for the development of a biofilm matrix. These bacterial biofilms pose higher natural resistance to conventional antibiotic therapy due to their multiple tolerance mechanisms. This prevailing condition has led to an increasing rate of treatment failures associated with P. aeruginosa biofilm infections. A better understanding of the effect of a diverse group of antibiotics on established biofilms would be nec...
The Brazilian Journal of Infectious Diseases, 2011
Biofilm production is an important mechanism for bacterial survival and its occurrence together with antimicrobial resistance represents a challenge for clinical management. Here, we evaluated the ability for biofilm production among P. aeruginosa isolates from patients with or without cystic fibrosis (CF) using two distinct media, besides determining the antimicrobial susceptibility profile of these isolates for eight antimicrobial agents. The ability for biofilm production when TSB medium was used was higher than when used CF sputum media (p = 0.0198). However, P. aeruginosa isolates from CF have demonstrated similar performance for biofilm production, independently of the medium used. Besides, among the biofilm-producing isolates, those recovered from CF were more resistant to the carbapenems (meropenem and imipenem) agents than those isolates from non-CF isolates.
The Iraqi Journal of Agricultural science, 2020
The current study was designed to explore the association between the pigments production and biofilm construction in local Pseudomonas aeruginosa isolates. Out of 143 patients suffering from burns, urinary tract infections (UTI), respiratory tract infections and cystic fibrosis obtained from previous study by Mahmood (2015), twenty two isolates (15.38%) were identified from (11) hospitals in Iraq, splitted into three provinces, Baghdad, Al-Anbar and Karbala for the duration of June 2017 to April 2018. Characterization was carried out by using microscopical, morphological and biochemical methods which showed that all these isolates belong to P. aeruginosa. Screening of biofilm production isolates was carried out by using nutrient broth supplemented with glucose (0.25%) production medium which encourage this biofilm production. The percentage of pigmented isolates were collected from a total of 143 samples, 2.8% of the isolates from burns, 2.1% isolates from cystic fibrosis and 0.7% isolates from UTI. Quantitative assays for biofilm formation were conducted using ELIZA technique. The results showed that all (22) isolates produced biofilm except one (B1 isolate). Biofilm quantities were varied from strong to medium production in comparison with control (0.0663). Statistical analysis results using Fischer's Exact test (p<0.05) were nonsignificant, therefore the pigment production has no association with biofilm formation for all of them.
IUG Journal of Natural Studies, 2019
Biofilm forming ability is a major virulence factor of most of pathogenic bacteria. Over 65% of health care associated infections are caused by biofilm producing organisms. The primary objective of this cross sectional study is to evaluate the biofilm forming ability of Pseudomonas aeruginosa isolated from various departments of Al-Shifa hospital and to determine the susceptibility of the isolates for antimicrobial drugs and acetic acid. Swabs moistened with sterile normal saline were used to swab an area of three cm 2 . Samples were cultured and processed by standard microbiological procedures. P. aeruginosa isolates were tested for their antimicrobial susceptibility patterns, the minimum inhibitory concentration for acetic acid and the biofilm forming ability were determined using Tube method and Crystal violet assay method. Susceptibility of P. aeruginosa isolates for acetic acid was considered as high at Conc. = 0.0097 mg/ml (5%), moderate susceptibility (0.156 to 0.019 mg/ml) ...
Detection of Biofilm Formation in Pseudomonas aeruginosa Isolates from Clinical Specimens
ZANCO Journal of Pure and Applied Sciences, 2018
The ability of a microorganism to develop biofilm is considered as a marker of clinically relevant infection. Infections caused by P. aeruginosa are difficult to treat as the majority of isolates exhibit high level of innate resistance to many antibiotics and tendency to form biofilms. The study was undertaken to investigate biofilm-forming capabilities of P. aeruginosa isolated from clinical specimens by two main different methods tissue culture plate (TCP) method, and congo red agar (CRA) method. Further, to investigate the antimicrobial resistance profile among biofilm producing isolates. A total of 300 specimens were collected from patients. Ninety six isolates of P. aeruginosa were obtained from various clinical samples. We applied Vitek-2 automated system as a panel of antimicrobial agents. TCP method and CRA method assay was chosen to detect the biofilm formation. Out of the 96 isolates, the results by TCP method and CRA method were 84(87.5%) and 76(79.1%) isolates respective...
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