Three different human tumor cell lines contain different oncogenes (original) (raw)
Related papers
New rat cell line that is highly susceptible to transformation by several oncogenes
Molecular and cellular biology, 1984
We describe here a new cell line, EL2, which spontaneously arose from primary rat embryo fibroblasts and has the distinctive property of being highly susceptible to a number of different transforming genes. The high susceptibility is expressed not only in high transformation frequencies but, most importantly, in an unusually high rate of growth of EL2 transformants under selective conditions, i.e., in soft agar or as foci. The biological characteristics of EL2 cells greatly accelerate the isolation of transformants from known oncogenes and could be useful to detect new transforming genes.
Activation of mouse genes in transformed cells
Cell, 1983
We have used molecular hybridization and cDNA cloning techniques to isolate mouse cellular genes activated in SV4tMransformed cells and we show that many of the clones belong to one of four sets. We characterize the cytoplasmic transcripts and genomic sequences homologous to two of these sets. The Set 1 transcription unit(s) is activated in all SV40-transformed cell lines analyzed, and experiments with fsA-mutant-transformed lines show that activation appears to require functional large T-antigen. This transcription unit(s) is also activated in mouse fibroblasts transformed by other agents, including retroviruses and chemical carcinogens. Activation of the Set 2 transcription unit(s) is more restricted, being confined to cell lines transformed by SV40 and retroviruses with distinctive biological properties.
Transformation of mammalian cells with an amplifiable dominant-acting gene
Proceedings of the National Academy of Sciences, 1980
We have transferred a mutant hamster gene coding for an altered dihydrofolate reductase to wild-type cultured mouse cells by using total genomic DNA from methotrexate-resistant Chinese hamster ovary A29 cells as donor. By demonstrating the presence of hamster gene sequences in transformants we have provided direct evidence for gene transfer.
Transformation of human cells by DNAs ineffective in transformation of NIH 3T3 cells
Proceedings of the …, 1985
Neonatal human foreskin fibroblasts can be transformed to anchorage-independent growth by transfection with DNAs inefficient in transforming NIH 3T3 cells. Human cells transfected with DNA from GM 1312, a multiple myeloma cell line, or MOLT-4, a permanent lymphoblast line, grow without anchorage at a much higher frequency than do the parental cells and their DNAs can transform human cell recipients to anchorage-independent growth; they have extended but not indefinite life spans and are nontumorigenic. Human fibroblasts are also transformed by DNAs from two multiple myeloma lines that also transform 3T3 cells; however, restriction analysis suggests that different transforming genes in this DNA are acting in the human and murine systems. These results indicate that the human cell transfection system allows detection of transforming genes not effective in the 3T3 system and points out the possibility of detection of additional transforming sequences even in DNAs that do transform murine cells.
Passage of phenotypes of chemically transformed cells via transfection of DNA and chromatin
Proceedings of the National Academy of Sciences of the United States of America, 1979
DNA was prepared from 15 different mouse and rat cell lines transformed by chemical carcinogens in vitro and in vivo. These DNAs were applied to NIH3T3 mouse fibroblast cultures by using the calcium phosphate transfection technique. DNAs of five donor lines were able to induce foci on the recipient monolayers. Ten other donor DNAs yielded few or no foci. DNAs from control, nontransformed parental cell lines induced few or no foci. Chromosomes were transfected from one donor whose naked DNA was unable to induce foci, and morphologic transformation of recipients was observed. These experiments prove that in five of these cell lines the chemically induced phenotype is encoded in DNA, and the sequences specifying the transformed phenotype behave as a dominant allele in the NIH3T3 recipient cells. The sequences encoding the transformation are likely found on a single fragment of DNA.
Proceedings of the National Academy of Sciences, 1981
We transformed BALB/3T3 mouse cells with cellular DNA extracted from the Meth A sarcoma, a 3-methylcholanthrene-induced tumor ofBALB/c mice, and asked whether foci arising in the transfection possess the previously defined Meth A tumor-specific transplantation antigen (TSTA). Five of eight foci selected from one experiment possessed Meth A TSTA. DNA extracted from one of the five TSTA-positive clones was used in secondary rounds oftransfection transformation. Four out of five foci tested from the secondary transfections possessed Meth A TSTA. These results suggest that in the Meth A sarcoma a transforming gene and a genetic determinant of TSTA are intimately related: they may be identical or very closely linked; alternatively, a particular transforming gene might induce the expression of a particular TSTA. Another possible explanation for these results is that the cotransfer of certain cellular genes by DNA transfection is considerably higher than predicted from the limited studies presently available.