Electron microscopic analysis of the transcription of the Bacillus subtilis riboflavin operon inserted into the hybrid plasmid pLP102 (original) (raw)

We used electron microscopy and computer analysis to study the ~aciil~ ~b~~~~ ~bofla~n opcron transcription. The size of the ~~~avin opcron has been shown to be 2.4Ort 0.15 MDa. Tran~~ption maps of the riboflavin operon in vitro and in viva have been obtained. In the riboflavin operon region four tran-.%xipts are sythesizcd in vivo having the following approximate boundary positions in the map of pLPIO2: 46-52,46-72, 62-72 and 55-58 map units. The riboflavin operon is transcribed from three promoters, at 46,58 and 72 map positions. A scheme of the riboflavin operon transcription in B. subtih is suggested.