Ribosomes of rat liver catalyze ‘minimal’ donor reaction (original) (raw)

Rat liver ribosomes were shown to catalyze peptide bond formation using 2'(3')-O-(N-formylmethionyl) adenosine 5'-phosphate (pA-(fMet)) as a peptide donor. The ribosomes were isolated from rat liver homogenate and subjected to various conditions to assess their transferase activity. The results indicate that rat liver ribosomes, while less efficient than E. coli ribosomes, effectively catalyze the reaction with Phe-tRNA, further demonstrating structural similarities in the peptidyltransferase donor sites of procaryotic and eucaryotic ribosomes.