Elevated Sweat Chloride Level in Children Without Cystic Fibrosis Receiving Topiramate or Zonisamide (original) (raw)

"Background: Topiramate and zonisamide are used as anticonvulsant medications in children, and Topiramate is also used for migraine prophylaxis. There are reports of topiramate and zonisamide causing oligohydrosis as a side-effect (Clin Neuropharmacol 2008; 31: 339-346, Pediatr Neurol 2003; 28: 184-189), but their effect on sweat chloride levels has not been studied systematically (Indian Pediatr 2008; 45: 238-240). Hypothesis: Topiramate and zonisamide not only reduce sweat production, but they can also cause elevation of sweat electrolytes. Methods: Eighteen patients who were receiving topiramate or zonisamide therapy for at least 6 months were enrolled either from Neurology outpatient clinic or Epilepsy Monitoring Unit, and were only included in the study if they had no signs or symptoms and a negative family history of cystic fibrosis. Patients underwent pilocarpine iontopheresis and sweat collection via Macroduct system (Wescor Inc., Logan, UT) as per Clinical and Laboratory Standards Institute (CLSI) and Cystic Fibrosis Foundation (CFF) guidelines. The samples were analyzed the same day using a chloridometer and the mean sweat chloride values were compared to published normative data (J Pediatr 2008; 153: 758-63) by Student’s t-test. Results: Of the 18 pts (age range 3-22 years, mean age 9.94 years, sex ratio M:F = 0.44) that were enrolled for this study, 14 adequate samples (>15 µL volume) could be obtained from left arm, and 13 from right arm. There were 14 patients on topiramate and 4 were receiving zonisamide. The mean sweat chloride level was 41.77 ± 18.71 mEq/L from the left arm and 46.475 ± 19.29 mEq/L from the right arm for patients receiving topiramate. For patients on Zonisamide therapy, the mean sweat chloride level was 29.83 ± 10.00 mEq/L from the left arm, and 36.5 ± 3.54 mEq/L from the right arm. Sweat chloride values and volumes did not correlate with dose of either anticonvulsant (p=0.5). Overall 7/14 (50%) patients on topiramate or zonisamide had either a borderline (> 40 mEq/L but < 60 mEq/L) or elevated (> 60 mEq/L) sweat chloride test result. Comparing these results to the standard reference values for sweat chloride in the normal population, the mean sweat chloride level of patients on topiramate was noted to be significantly higher (p<0.001) for both arms. There was no significant difference in the mean values for sweat chloride between the topiramate and zonisamide groups for both sides (p>0.05). The insufficient sweat collection rate was 3/18 (17%) for both arms, and another 3/18 had insufficient sweat collected from one of the arms. This rate is much higher than the goal of <5% inadequate sweat collection rate that is established by CFF for accredited sweat testing laboratories, and suggests that drug-induced oligohydrosis is operative in this patient population. Conclusions: Topiramate and zonisamide can not only cause oligohydrosis, but they can also elevate sweat chloride levels in patients with no clinical features of cystic fibrosis. Further studies to explore whether this is due to interaction with CFTR or via a different mechanism, are underway."

Thirty Years of Sweat Chloride Testing at One Referral Center

Frontiers in pediatrics, 2017

To conduct a descriptive analysis of the sweat test (ST), associating ST results with epidemiological data, CFTR (cystic fibrosis transmembrane conductance regulator) mutations and reasons to indicate the ST, as well as correlating sweat sodium and sweat chloride concentrations in subjects. Retrospective survey and descriptive analysis of 5,721 ST at a university referral center. The inclusion of the subjects was based on clinical data related with cystic fibrosis (CF) phenotype. The samples were grouped by (i) sweat chloride concentrations (mEq/L): <30: 3,249/5,277 (61.6%); ≥30 to <60: 1,326/5,277 (25.1%); ≥60: 702/5,277 (13.3%) and (ii) age: (Group A--GA) 0 to <6 months; (Group B--GB) ≥6 months to <18 years; (Group C--GC) ≥18 years. Digestive symptoms showed higher prevalence ratio for the CF diagnosis as well as association between younger age and higher values of sweat chloride, sweat sodium, and chloride/sodium ratio. The indication of ST due to respiratory symptoms...

Topiramate reduced sweat secretion and aquaporin-5 expression in sweat glands of mice

Life sciences, 2007

Decreased sweat secretion is a primary side effect of topiramate in pediatric patients, but the mechanism underlying this effect remains unclear. This study aimed to better understand how topiramate decreases sweat secretion by examining its effect on the expression of carbonic anhydrase (CA) II and aquaporin-5 (AQP5), total CA activity, as well as on tissue morphology of sweat glands in mice. Both developing and mature mice were treated with a low (20 mg/kg/day) and high dose (80 mg/kg/day) of topiramate for 4 weeks. Sweat secretion was investigated by an established technique of examining mold impressions of hind paws. CA II and AQP5 expression levels were determined by immunofluorescence and immunoblotting and CA activity by a colorimetric assay. In mature mice, topiramate treatment decreased the number of pilocarpine reactive sweat glands from baseline in both the low and high dose groups by 83% and 75%, respectively. A similar decrease was seen in developing mice. Mature mice w...

Hypohidrosis and hyperthermia during topiramate treatment in children

The Turkish journal of pediatrics

Topiramate is one of the newer generation antiepileptic drugs with a beneficial clinical effect on various seizure types. In this study, we present the clinical findings of hypohidrosis and hyperthermia with topiramate in pediatric patients. The data were collected retrospectively on 173 patients diagnosed as epilepsy on topiramate treatment, and hypohidrosis-related symptoms induced by topiramate were found in 22 patients. Their mean age was 64.45 +/- 56.63 months. The mean duration of topiramate treatment was 7.09 +/- 2.46 months, and the mean dose was 5.37 +/- 1.75 mg/kg/day. All of the patients complained of hypohidrosis and hyperthermia. Six (27.2%) of them had facial flushing, 4 (18.1%) had heat sensation and only 1 (4.5%) had lethargy. Hypohidrosis-related symptoms resolved after discontinuation of the medication. In conclusion, children treated with topiramate should be cautioned regarding these potential adverse effects and advised to avoid its use during the hot summer sea...

Topiramate associated hypohidrosis and hyperthermia

Indian pediatrics, 2008

Topiramate is a new antiepileptic drug, used for treatment of partial onset seizure and refractory seizures. Although it is well tolerated in children, some adverse effects including hypohidrosis and hyperthermia are reported. We present two children with epilepsy who were treated with topiramate and developed hypohidrosis and hyperthermia.

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