Effect of three anthocyaninless genes on germination ability of the brown seed (bs) mutant in tomato (original) (raw)

Seed Science and Technology, 2012

Abstract

ABSTRACT The effect of three mutated genes affecting anthocyanin biosynthesis in tomato (anthocyaninless of Hoffmann - ah, anthocyaninwithout - aw and baby lea syndrome - bls) on germination capacity of the brown seed (bs) mutant was investigated. Mutations brown seed ( bs, bs 2, bs 4) controlling darkness of the seed testa are known to have a negative impact on the velocity of seed germination. In contrast, the three anthocyaninless mutants, characterised by absence of condensed tannins in the testa inner epidermal layer were found to enhance germination under optimal and stress conditions. The study was performed on a series of isogenic/near isogenic lines (IL/NILs) as follows: Ailsa Craig (AC), AC bs, AC ah, AC aw, AC bls, AC ah bs, AC aw bs and AC bls bs. Under optimal conditions, low temperature and salt stress, the anthocyaninless IL/NILs possessing brown seeds germinated more rapidly than the seeds of the anthocyanin-containing bs line (AC bs), the differences in time to 50% germination being significant. The histochemical tests of the seed coat provided evidence that, as in ah, aw and bls mutants, no condensed tannins were detected in the testa of double-mutant seeds. We concluded that the absence of condensed tannins in the testa of the brown seeds contributed to their more rapid germination.

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