Synthesis, characterization and evaluation of antibacterial efficacy of zinc oxide nanoparticles (original) (raw)
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Antimicrobial Activity of Zinc Oxide Nanoparticles
The purpose of this review is to summarize published data regarding synthesis and antimicrobial behaviour of zinc oxide nano particles. Zinc oxide is low cost, nontoxic, exhibit outstanding optical, physical, photocatalytic, and antimicrobial properties. Antimicrobial behaviour depends on shape and size of nano particles. Toxicity of zinc oxide nano particles toward both gram positive and gram-negative bacteria make them a potential antimicrobial agent. Due to this, zinc oxide nano particles are well accepted in the field of cosmetics, medicine, textile, food packaging etc. This paper reviews method of synthesis and application of synthesized material as anti-microbial agent. Zinc oxide nano particles are very much effective against Campylobacter jejuni, A. baumannii, S. aureus, S. Typhimurium, methicillin resistant S. aureus, Salmonella typhi and many more. They inhibit the growth of microorganisms by causing oxidative stress and by permeating into the cell membrane.
Synthesis of zinc oxide nanoparticles and evaluated its activity against bacterial isolates
2020
Zinc oxide nanoparticles (ZnO NPs) have acquired large attention against microbial activity because of their distinctive properties associated with their size and shape. In current research, ZnO NPs produced by the Sol-gel method at room temperature were applied to evaluate its antibacterial activity against pathogenic bacterial strains of Staphylococcus aureus and Escherichia coli obtained from Al-Sadr Hospital, Maysan, Iraq. The prepared nanoparticles were investigated by dynamic light scattering (DLS), X-ray diffraction (XRD), and scanning electron microscopy (SEM). Antibacterial activity was tested by using the agar diffusion method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) after 24 h of exposure to prepared ZnO NPs. The size distribution, characterization, and morphology of nanoparticles showed around 100 nm as observed by DLS, and the formed nanoparticles were spherically confirmed by SEM. Additionally, results of the disc diffusion ...
ANTIBACTERIAL ACTIVITY OF ZNO NANOPARTICLES ON S.AUREUS AND E.COLI.
In this study, the effects of zinc oxide (ZnO) nanoparticles (NPs) on, Gram?ve bacteria (E.coli ) and Gram+ve bacteria (S.aureus) were investigated. The bacterial strains were inoculated into media containing different concentrations of ZnO (16, 8, 4, 2 m M) and 1% of NP-free solution and incubated at 37 ?C for 24 h. The presence and characterization of ZnO nanoparticles on bacterial cells were investigated by scanning electron microscopy (SEM)The bacteria, for up to 12 h of incubation, the numbers of treated cells were within 1 log CFU/mL less than that of the control.Morphological changes of bacterial cells were observed, but many cells remained in normal shapes. Results indicate that ZnO nanoparticles has very inhibitory effects on bacteria.
Antibacterial activity of ZnO nanoparticle on Gram-positive and Gram-negative bacteria
African Journal of Microbiology Research, 2012
The aim of the present study is to determine the antimicrobial activity of ZnO nanoparticles against Gram-negative and Gram-positive bacteria. Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were used as test microorganisms. The effects of particle size and concentration on the antibacterial activity of ZnO nanoparticles was studied using bacteriological tests such as disc and well diffusion agar methods, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). These tests were performed in nutrient broth and nutrient agar following standard methods. In addition, the effect of different concentrations of ZnO nanoparticles on the growth of E. coli and S. aureus was measured with respect of time. The minimum inhibitory concentration was determined using seven different concentrations of ZnO nanoparticles including 16, 8, 4, 2, 1 and 0.5 mg/ml. The MIC value for E. coli and S. aureus was 1 and 0.5 mg/ml, respectively. The results showed that ZnO nanoparticles have antibacterial inhibition zone of 29 and 19 mm at the concentration of 10 mg/ml against E. coli and S. aureus, respectively. Gram-negative bacteria seemed to be more resistant to ZnO nanoparticles than Gram-positive bacteria. It was found that the antibacterial activity of ZnO nanoparticles increased with decreasing particle size and increasing powder concentration. The antibacterial effect of ZnO nanoparticles was time dependent and takes effect gradually. ZnO bulk powder showed no significant antibacterial activity.
Journal of Applied Biotechnology Reports, 2018
Introduction: Recent increases in microbial resistance to multiple antibiotics have led to the emergence of more economical methods for producing nanoparticles with physical, chemical effects and limited resistance. The aim of this research was to study zinc oxide (ZnO) nanoparticles synthesis and antibacterial properties against some gram-negative and gram-positive bacteria. Material and Methods: In this study, ZnO nanoparticle was synthesized using ultrasonic method and bioassayed on 10 human pathogenic bacteria by agar well diffusion method. In addition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined as well. Antibiotic resistance pattern of bacteria was determined to 9 antibiotics: gentamicin, ampicillin, nalidixic acid, amoxicillin, amikacin, ciprofloxacin, co-trimoxazole, norfloxacin and cephalexin by disc diffusion assay. Results: The nanoparticles were synthesized with suitable morphology and distribution. All gram-positive and gram-negative bacteria were inhibited at the low concentration of ZnO nanoparticles most bacteria had resistance to antibiotics. Conclusions: The findings suggest that the ZnO nanoparticles have potential applications as antibacterial compounds and their mechanism of action is dependent upon composition and surface modifications.
REVIEW OF THE ANTIBACTERIAL PROPERTIES OF ZnO NANOPARTICLES
Journal of the Maharaja Sayajirao University of Baroda , 2022
Developing antibiotic resistance in bacteria is one of the most pressing issues in world health care. The utilisation of metal nanoparticles and their oxides is a promising strategy for overcoming antibiotic resistance. Numerous research has demonstrated the remarkable antibacterial activity of zinc oxide nanoparticles (ZnO-NP) in respect to gram-positive and gram-negative bacteria, in particular Zinc oxide nanoparticles have been studied for their antibacterial properties in recent years, and the results of these investigations have been summarised in this mini-review. The antibacterial activity on E. coli and S. aureus is dependent on the size of the applied nanoparticles. Zinc oxide nanoparticle production methods and the most common modifications of NP-ZnO to improve antibacterial activity are also examined.
Background and Objectives: Along with the rapid development of human life, controlling harmful effects of microorganisms would be unavoidable. The objective of this study was to evaluate antibacterial efficacy of zinc oxide nanoparticles on different microbial strains. Material and Methods: This experimental study was done using gram negative and gram positive bacteria in nutrient media. Nanoparticle characterization was determined using X-ray diffraction)XRD(, scanning and transmission electron microscopy)SEM and TEM(. Bacterial sensitivity to nanoparticles was tested using a disk diffusion test and minimum inhibitory concentration)MIC(. Time-kill studies and other tests were carried out using 108 CFU/mL of bacteria at baseline. A point of zero charge, pHzpc, of nanoparticle was investigated using the batch equilibration method. Obtained data were managed by SPSS Ver.16 and were analyzed through the Pearson, analysis of variance (ANOVA) and Student's independent t-tests. 0.05 was selected as significant level for all tests. Results: Characterization results from XRD, SEM, and TEM showed that particles are in nano range and they do not contain any discernible crystalline impurity. The average ZnO nanoparticles diameter was 20 nm. The pHZPC for ZnO was found to be 7.51. The P. aeruginosa strain exhibited larger diameter inhibition zone)DIZ(to ZnO nanoparticle compared with other strains. Population of P.aeroginosa for 2 x MIC concentration was reduced to zero in the presence of nano ZnO within 150 min. The bacterial CFU had significant difference with contact time, nanoparticles loading, and bacterial strain)P<0.001(. Conclusion: This study demonstrated that antibacterial activity of ZnO can be a candidates for the elimination of gram negative and gram positive bacteria, particularly in nasocomial infection agent control.
Preparation of ZnO and ZnS nanoparticles and in-vitro study of their antimicrobial effect
2017
Zinc sulfide (ZnS) & zinc oxide (ZnO) nanoparticles was evaluated for their antimicrobial activity against four pathogenic strains. ZnS&ZnO nanoparticles were synthesized by simple aqueous chemical reaction in an aqueous solution. The main advantage of these nanoparticles (size of 10-30 nm) was that simply could be prepared by using cheap precursors in a cost effective and high throughput manner. Structural, morphological and chemical composition of the prepared nanoparticles were investigated by X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and energy dispersion X-ray dispersive fluorescence spectroscopy (EDAX). The antimicrobial effects of the ZnS&ZnOnanoparticls were studied by serial dilution technique and also by well diffusion technique against four pathogenic microorganism strains of Staphyloccusaureus, Escherichia coli, Pseudomonas aeroginosa and Candidiaalbicans. Both nanoparticles of ZnS&ZnO showed antimicrobial activity against both Gram positive Staphyloccu...
Review Article on Inorganic Nanoparticles as Antibacterial Agents
International Journal for Research in Applied Science and Engineering Technology IJRASET, 2020
Pathogenic bacteria are those bacteria that produce illness. These bacteria can cause diseases and infections when they get in the body of a healthy host and begin to reproduce very fast in the host body. For these bacterial diseases, researchers have developed antibacterial agents to aid infectious diseases, which are designed as promising chemotherapeutic agents. But the superbugs are smarter to develop resistance phenomenon against almost all the available antibacterial agents & hence make them ineffective. So there is an unmet need to develop antibacterial which do not develop fast resistance & can kill the superbugs so as to save the humanity against the cruel clutches of these superbugs. In this review, we discussed the recently discovered novel antibacterial based on inorganic nanoparticles.
Nanotechnology has attracted a lot of attention from every generation: either its new or old generation. Biosynthesized nanoparticles have been used mainly because of their medicinal properties. An important medicinal property of these nanoparticles is their antibacterial behavior against Gram positive and Gram negative bacteria. ZnO nanoparticles of Arisaema tortuosum (Wallich) Schott, and Rhus parviflora Roxb., leaves extract were synthesized by biochemical (precipitation) method and characterized by X-ray diffraction and UV-Visible spectroscopic analysis. At concentration 200 µg/ml, their antibacterial activity was evaluated against both Gram positive (Staphylococcus aureus) and Gram negative (Pseudomonas aeruginosa) bacteria and the results showed the antibacterial activity order as: ZnO nanoparticles (R. parviflora) > ZnO nanoparticles (A. tortuosum).