Residual Effects of Sucrose and Hormonal Treatments of the Tuberization Medium on in Vitro Germination of Potato (Solanum tuberosum L.) Microtubers (original) (raw)
Related papers
Advances in Bioscience and Biotechnology, 2013
The microtuber is considered one of the most effective means of spreading basic materials, as well as transporting and preserving potato germplasm varieties. To define the optimal conditions for the potato microtuber in vitro germination of Aida, Atlas and Odessa varieties, the effects of temperature, physiological age and grade (size) were evaluated. The study conducted at three different temperature levels has demonstrated that the most favorable temperature for microtuber germination at a higher and faster germination rate was 25˚C, regardless of the variety. In addition, microtubers of large caliber, greater than 4 mm, germinate more quickly, with a higher germination rate, than smaller size ones (less than 4 mm) for all genotypes. For Atlas, Aida and Odessa varieties, a germination rate equal to 86.66%, 70% and 70% respectively, was obtained for microtubers with a caliber superior to 4 mm. Physiological age influences microtuber germination. The mean length of sprouts, reached after a 7 week incubation period, was more marked at "multiple sprout" and "branched sprout" stages than at a "monosprout" stage. The average length was 2.35 cm, 2.48 cm and 1.5 cm, respectively. Thus, it is necessary to plant microtubers at a "multiple sprout" stage to optimize their yield in plants and minitubers.
2008
The use of in vitro tubers (microtubers) as the final products of potato micropropagation, in addition to or rather than in vitro plantlets has several advantages in seed tuber production. Moreover, microtubers are important in germplasm storage and they are used as experimental tools in basic research and in in vitro selection of agronomically important characters. Their reliable production has, therefore, been widely studied under different in vitro growing conditions, with varying efficacy regarding the number, size and weight of microtubers. Microtubers exhibit different periods of dormancy depending on the genotype and on the in vitro conditions during tuberization. Generally, the use of growth regulators for tuberization does not allow the maximal expression of the tuberization capacity that is determined by the genotype, and therefore, the production of microtubers in sufficient quantity and size. As a result, there are later problems in breaking tuber dormancy. Although the growth responses between in vitro and field-grown tubers are very similar, there is the possibility for the production of in vitro tubers without using growth regulators by modifying in vitro environmental factors, such as light, temperature and mineral nutrition, among others. This review discusses the results of in vitro potato tuberization performed in hormone-free systems on solidified medium, including the effects of environmental factors, composition of medium, genotype and explants. Furthermore, we assess the effects and post-effects of tuberization conditions on the dormancy and sprouting characteristics of microtubers originating from these hormone-free systems.
Effects of Growth Regulators, Media and Explant Types on Microtuberization of Potato
American Journal of Potato Research, 2020
Microtubers (MT) are an innovative approach for nuclear seed production in potato (Solanum tuberosum L.). They are produced under in vitro conditions using different protocols. However, content and type of growth media as well as explant types significantly affect the MT production efficiency. This study was conducted to develop an efficient protocol for MT production by evaluating different growth regulators at different concentrations, types of growth media and explants in two separate experiments. In Experiment 1, the effects of six different growth regulators, and their three application rates on MT formation were compared. The effects of two growth media and three explant types on MT production were compared in Experiment 2. As a result, our studies indicated that cotton based liquid MS medium containing 0.1 mg L −1 Thidiazuron, and using whole plants as explant can be used for efficient MT production in potato. Resumen Los microtubérculos (MT) son un enfoque innovador para la producción de semilla nuclear en papa (Solanum tuberosum L.). Se producen bajo condiciones in vitro usando diferentes protocolos. No obstante, el contenido y tipo de medio de cultivo, así como los tipos de explantes, afectan significativamente la eficiencia en la producción de microtubérculo. Este estudio se condujo para desarrollar un protocolo eficiente para la producción de MT mediante la evaluación de diferentes reguladores de crecimiento a diferentes concentraciones, tipo de medios de cultivo y explantes en dos experimentos separados. En el experimento 1, se compararon los efectos de seis diferentes reguladores de crecimiento y sus tres niveles de aplicación en la formación de MT. En el experimento 2, se compararon los efectos de dos medios de crecimiento y tres tipos de explante en la producción de microtubérculos. Como resultado, nuestros estudios indicaron que el medio líquido MS con base de algodón con 0.1 mg L-1 de thidiazurón, y usando plantas completas como explante puede usarse para la producción eficiente de microtubérculo en papa.
Influence of potato genotypes on "in vitro" production of microtubers
Romanian Biotechnological Letters
In vitro" microtuberization represents the transitory phase from "in vitro" multiplication of a healthy material to on field growing. Microtuber production is an efficient method for obtaining a healthy material, through which the process of potato production is reduced with 3-4 years. In the same time the microtubers are important because they can be produced in any period of the year, they are easy to be transported and stored.. Microtubers of semi-early Romanian varieties (Christian and Roclas) and of early Dutch variety (Ostara) were obtained from potato micro-cutting cultures on Murashige-Skoog medium enriched with Cumarin and Kinetin. The sucrose was the most important stimulus for inducing the microtubers. Microtuber inducing and growing was achieved in cultures maintained in darkness, at 18-20° C for 8-10 weeks.
in Vitro Cellular & Developmental Biology-plant, 2004
With the objective of using microtubers for conservation of potato germplasm, the main effects of genotype, abscisic acid (ABA), and sucrose level, and of their interactions on biomass production, microtuberization, microtuber dormancy, and dry matter content, were studied. ABA decreased both microtuber production and microtuber dormancy, whereas higher concentrations (60–80 gl−1) of sucrose promoted biomass production, microtuber production as well as microtuber dry matter content. Microtubers stored under diffused light had longer dormancy than those kept continuously in the dark. Interactions among various factors conditioned the main effects for some characters. In vitro performance of the genotypes studied was related to their known performance under in vivo conditions for most of the characters. Microtubers produced on media devoid of ABA and containing high sucrose concentrations and N6-benzyladenine (44.38 μM) could be stored for 12 mo. under diffused light at 6±1°C.
2018
The present experiment was aimed to study the effect of sucrose and growth regulators (kinetin and 6benzylaminopurine) on potato microtuber formation. For this purpose the Murashige and Skoog (MS) media, supplemented with different levels of sucrose concentration and growth regulators had been used. The result showed that MS medium with double level (60g) of sucrose without any growth regulator had significantly enhanced the tuber formation but T1 treatment (i.e.100g of sucrose) had no significant effect on tuber formation. It was revealed that sucrose had positive impacts on the microtuberization up to some extent but at very high concentration reduced the microtuber formation. The kinetin and 6benzylaminopurine (BAP) also enhanced the microtuber formation. Early tuber initiation was observed in kinetin treated plants as compared to other treatments and BAP treated plants showed increase in weight of microtubers. The study also revealed that increase in density of plantlets had neg...
Varietal performance of potato on induction and development of microtuber in response to sucrose
Annals of Agricultural Sciences, 2017
Tissue culture techniques are the most momentous and extensively method to produce disease-free quality plantlets and microtubers in potato. In spite of this, there has been a lack of research information into in vitro microtuberization for seed tuber production program in Bangladesh. In this experiment, we assessed the appropriateness of different concentrations of sucrose with especial accent on their effects for induction and development of microtuber in potato varieties. In vitro plantlets of three potato varieties; Asterix, Granola, and Diamant were treated with eight level of sucrose as 0, 3, 4, 6, 8, 10, 12 and 14% for 70 days of incubation producing microtuber. Asterix induces microtuber after 10.69 days, it was statistically shorter duration than other two varieties. Tuberization did not occur without sucrose and were required a minimum (8.92 days) with 8% sucrose, while it delayed with either increasing or decreasing rate of sucrose concentration. A single number of microtuber was not formed in absence of sucrose after 28 days of incubation. Microtuber plantlet À1 was the highest more or less in all varieties at harvest with 8% sucrose concentration. Hundred percent of microtuber below 250 mg induced in 3% sucrose concentration and from then the microtuber grade induction decreased with the increase of sucrose concentration. >500 mg grade microtuber produced by Granola (47.95%) with 10% sucrose while Diamant produced 50.15% above 500 mg grade microtuber with 14% sucrose. It is also noticeable by Astrerix variety where >500 mg microtuber were produced about 46.95% with 8% sucrose.
Acta Scientifica Naturalis, 2020
This work investigates the interactive effect of six culture media and three photoperiods (darkness, 16h/8h, 8h/16h) on the microtuberization of four potato varieties (Spunta, Désirée, Kondor and Bartina). The objective is to determine the best tuberization under these growing conditions. The measured parameters which are related to the suitability of the tuberization characteristics are: the morphological aspects (shape, position) and the biometric ones (number and diameter of tubers). The obtained results permit to valorize the meristems that constitute the starting explant, and their good organogenetic skills to provide the first generation of micropropagation with healthy vitroplants in a sufficient quantity. Thus, the grown vitroplants on the medium (MS/2+BAP+COU) presented the best values which are related to the percentage of tuberization, the number of microtubercles / vitroplants and weight of tubers. Moreover, the Bartina genotype showed a remarkable superiority over its m...
Azarian Journal of Agriculture
The present work was conducted to evaluate the varietal performance of three potato varieties namely-Asterix, Granola and Diamant with different microtubers weights of >500 mg, 250-500 mg and <250 mg on sprout characters. The variety Granola showed longer dormancy period (30.33 days) in the case of less weight microtuber and it was decreasing the rate with increasing of microtuber weight in all varieties. The variety Diamant produced slightly more sprout per microtuber for all weights while the variety Asterix showed higher number of sprout per microtuber by >500 mg. Asterix had significantly longer sprouts (28.43 mm) than other two varieties and the trend of the length of sprout was decreased with the decrease of microtuber weight. The larger microtubers (>500 mg) of the variety Diamant and Asterix tended to have higher values on fresh weight of sprouts and sprout mass per unit of sprout length than Granola variety.