Copper ion removal by Thiobacillus ferrooxidans biomass (original) (raw)
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Studies on multi-metal ion tolerance of Thiobacillus ferrooxidans
Minerals Engineering, 1997
The influence of different concentrations of base metal ions, such as Cu 2+, Zn 2+ and Fe 3+, when present either alone or in different possible binary and ternary combinationa in a 9K medium, on the ferrous ion oxidation ability of Thiobacillus ferrooxidans was studied. Levels and degree of toxicity of these ions have been quantified in terms of toxicity index (TI). Copper and zinc tolerant strains of the bacteria were developed through .,wrial subculturing and their activity tested in the presence of the above metal ions in comparison with the behavior of wild unadapted cells under similar conditions. Copper tolerant strains (25 g/L Cu 2+) were found to be more efficient in the bioleaching of both copper and zinc concentrates than wild unadapted strains, while zinc tolerant strains (,40 g/L Zn 2+) exhibited better leaching efficiency only in the bioleaching of sphalerite concentrates. The significance and relevance of multi-metal ion tolerance in ThiobaciUus ferrooxidans has been highlighted with respect to bioleaching of sulphide mineral concentrates.
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2000
Respirometric experiments demonstrated that the oxygen uptake by Thiobacillus ferrooxidans strain LR was not inhibited in the presence of 200 mM copper. Copper-treated and untreated cells from this T. ferrooxidans strain were used in growth experiments in the presence of cadmium, copper, nickel and zinc. Growth in the presence of copper was improved by the copper-treated cells. However, no growth was observed for these cells, within 190 h of culture, when cadmium, nickel and zinc were added to the media. Changes in the total protein synthesis pattern were detected by two-dimensional polyacrylamide gel electrophoresis for T. ferrooxidans LR cells grown in the presence of different heavy metals. Specific proteins were induced by copper (16, 28 and 42 kDa) and cadmium (66 kDa), whereas proteins that had their synthesis repressed were observed for all the heavy metals tested. Protein induction was also observed in the cytosolic and membrane fractions from T. ferrooxidans LR cells grown in the presence of copper. The level of protein phosphorylation was increased in the presence of this metal.
Iron and Sulfur Oxidation byThiobacillus Ferrooxidansand Tolerance to Copper and Zinc Ions
Biotechnology & Biotechnological Equipment, 1997
Three strains of Thiobacillus ferrooxidans have been isolated from copper mine in Bulgaria, and investigated. The levels of their ferrous-and sulfur-oxidizing activities have been tested as well as their tolerance to copper and zinc ions. The effect of both metal ions on ferrousoxidizing activity and oxygen uptake have been investigated too. A possible mechanisms for the tolerance of these bacteria to Zn 2 + and Cu 2 + ions is discussed.
Biosorption of Zn(II) and Cu(II) by the indigenous Thiobacillus thiooxidans
Chemical Engineering Journal, 2004
Biosorption of each of the heavy metals, Zn(II) and Cu(II), and of the binary mixture of these two metal ions by the indigenous Thiobacillus thiooxidans was investigated in this study. Equilibrium concentration (q m) and dissociation constant (K d) were calculated by fitting the experimental data with the Langmuir isotherms. The effects of pH, pretreatment of biomass, and temperature on the amount of metal uptake by this organism were also determined. Typically, the adsorption capacity increases with increasing pH in the ranges of 2.0-6.0 and 4.0-5.0 for Zn(II) and Cu(II), respectively. Chemical pretreatment of the biomass with 0.075 M NaOH has positive effects on its capacity for metal biosorption. Higher temperature yields higher biosorption capacity for both metals. The indigenous T. thiooxidans is in favor of Zn(II) uptake in the binary mixture. Biosorption of Cu(II) is inhibited by the existence of Zn(II). The total amount of metal adsorbed in the binary mixture decreases in comparison with biosorption of only one kind of metal ion.
Heavy metals removal by bioleaching using Thiobacillus ferrooxidans
2017
This paper presents research results obtained on laboratory column reactor filled in with soil sampled in natural state by bioleaching of contaminated soils with metals. In the first step of experiments (3 months of treatment), the concentration of metals (Pb, Cu, Zn, Mn) were determined from soil and leachate samples by inductive coupled plasma atomic emission spectrometry. Then the research was focused on a comparative study regarding the concentrations of pollutants in soil. In this condition were determined: the initial amount of metal in soil, the amount of metal in the natural state soil at the end of treatment by bioleaching and the amount of metal after a month of extraction process (post-treatment). This research has led to the conclusion that the natural state soil may be treated by in situ bioleaching, after 3 months of treatment high extraction yields being obtained (Pb 78–90%; Cu 68–84%; Zn 74–90%; Mn 85–95%).
THE TOLERANCE OF THIOBACILLUS FERROOXIDANS BACTERIA TO DIFFERENT CONCENTRATIONS OF FERROUS SULPHATE
A special importance for using bacteria of the genus Thiobacillus in the biosolubilization processes of heavy metals from acid mine tailings is represented by the resistance of these microorganisms to high concentrations of metal ions. Due to the toxic increased concentrations of metal ions the study of the tolerance of chemolithotrophic acidophilic bacteria potentially involved becomes necessary in biotechnological applications. Therefore, the increasing efficiency of the bioleaching processes implies the stability of the tolerance of chemolithotrophic acidophilic bacteria to heavy metals and also the selecting of strains aand populations with an increased resistance. In this context, in the present paper was pursued the study of tolerance to high concentrations of ferric iron (10-20g/l Fe 2+ ) of the bacteria of the genus Thiobacillus. At the concentrations of 10 and 12g/l Fe 2+ the bacterial populations had an intense metabolical activity, oxidating the whole amount of Fe 2+ in 6 days and at the concentrations of 14, 16g/l Fe 2+ the iron in the medium was oxidated in a longer period of time.
Journal of Physics: Conference Series, 2019
This research was conducted to remove heavy metal Cu2+ using bioremediation method with the utilization of mix culture of bacterium Thiobacillus sp and Clostridium sp. It started with the cultivation of artificial liquid growth media, Stone Mineral Salt solution (SMSs) using temperature (oC) and pollutant concentration of heavy metal Cu2+ (ppm) as the test parameters. The analysis was conducted with Atomic Absorption Spectrophotometry (AAS) to determine the concentration of heavy metal Cu2+ at the beginning and end of the research in order to calculate its removal efficiency. The exponential phase of mix bacterial culture growth was observed on day 5 with a pH value of 7. Furthermore, the culture was discovered to have the ability to live in several temperatures (oC) of 20, 25, 30 and 35, and remove heavy metal Cu2+ at 89.10%; 91.27%; 92%; and 90.27% respectively. The results also showed that at a contact time of 48 hours, the temperature of 30oC, and Cu2+ pollutant concentration of...