Physico- chemical investigation and antioxidant activity studies on extracts of Eruca sativa seed (original) (raw)
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E. sativa is a member of the Brassicaceae, which is considered to be an important chemopreventive plant family. The total polyphenols and flavonoids contents of E. sativa meal and oil have the highest of total polyphenols and flavonoids contents, which were 49.77 mg GAE/g and 11.82 mg GAE/g, respectively. Antioxidant activity of methanolic, hexane and heat-methanolic extracts and oil of E. sativa were determined by using FRAP, ABTS, and DPPH. The methanolic extract meal and oil of E. sativa have the highest reducing power which was ranged from 0.8925 to 2.0191 and 0.7596 to 1.4765 at the concentrations of 10 and 80 mg/ml, respectively. Also, by used (ABTS) it could be percentage were 72.19% (methanolic extract) and 65.23% (oil), While, by used (DPPH) it could be percentage of inhibition (IC 50) which were 0.059 to 0.041 for methanolic extract and 0.047 to 0.025 for oil at the concentrations of 250 and 1000 µg/ml, respectively.
Phytochemical analysis and antibacterial activity of Eruca sativa seed
Pak. J. Bot, 2011
Antibacterial activity of various solvent extracts of Eruca sativa seed as well as seed oil was investigated against Gram+ve and Gram-ve bacterial strains. Maximum zone of inhibition was observed from seed oil followed by methanolic seed extracts from all bacterial strains compared with broad spectrum antibiotics gentamicine. MIC values of seed oil were within the ranges of 52-72 µg/ml as compared to 56-70 µg/ml standard antibiotic (Gentamicine). Proximate and Phytochemical analysis of seed of E. sativa showed presence of all essential phyto constituents required for promising traditional medicine. Analysis of seed oil by gas chromatography revealed that there was high concentration of Erucic acid (51.2%) followed by oleic acid (15.1%) and cis-11-eicosenoic acid (12.5%). In addition, minor quantities of other essential and non essential fatty acids were also present. Therefore the present study supports effectiveness of E. sativa seeds for its use in traditional medicine used in various human disorders.
Food Chemistry, 2010
The present study investigates the antimicrobial activity of various solvent extracts of Eruca sativa (aerial and root) and seed oil against-antibiotic resistant Gram-negative (Escherichia coli, Pseudomoms aeruginosa and Shigella flexneri) and Gram-positive (Staphylococcus aureus and Bacillus subtilis) bacteria. Among the various preparations, seed oil was the most active, exhibiting a maximum zone inhibition of 97% for Gram-positive bacteria and of 74-97% for Gram-negative bacteria. The MIC of the seed oil was found to be 65-75 and 60-70 lg/ml for Gram-negative and Gram-positive bacteria, respectively. Analytical investigation on main volatile and non-volatile components was performed on seed oil. Among the formers allyl isothiocyanate (40 lg/g), 3-butenyl isothiocyanate (260 lg/g), 4-methylsulfinybutyl isothiocyanate (sulforaphane 743 lg/g), 2-phenylethyl isothiocyanate (159 lg/g) and bis(isothiocyanatobutyl)disulphide ($5000 lg/g) were determined by head space/SPME/GC-MS analysis. Free fatty acids were 1.6% w/w of the oil and overall 25 fatty acids were identified. Erucic and oleic acids were the main fatty acids both in the free (7.8 and 2.1 mg/ml) and esterified forms (50.6% w/w and 14.9% w/w of total fatty acids). Unsaponifiable fraction was 1.8% w/w. Seed oil of E. sativa has promising pharmacological efficacies and ensures the presence of bio-active components responsible for the observed beneficial effects. Our findings support its use in traditional medicine as antimicrobial bioagent and highlight the potential of this food plant for its possible clinical use.
Journal of Medicinal Plants Research, 2011
Phytochemical investigations of the aqueous extract of Eruca sativa fresh leaves, afforded the presence of nine natural flavonoid compounds which were isolated and identified as kaempferol 3-O-(2''-Omalonyl-β-D-glucopy-ranoside)-4'-O-β-D-glucopy-ranoside (1), kaempferol 3,4'-O-diglucopyranoside (2), rhamnocitrin 3-O-(2''-O-methylmalonyl-β-D-glucopyranoside)-4'-O-β-D-glucopyranoside (3), 3-Oglucopyranoside (4), 4'-O-glucopyranoside (5), rhamnocitrin 3-O-glucopyranoside (6), 4'-Oglucopyranoside (7), kaempferol (8) and rhamnocitrin (9). Compounds (1) and (3) appear to be novel. Elucidation of the chemical structures of all the isolated compounds was determined by different spectroscopic methods in addition to the chemical and physical methods of analysis. In vitro antitumour study of E. sativa 70% ethanolic extract (ES-EE) as well as Compounds (1) and (3) proved their cytotoxic activity in 4 different human tumor cell lines: HepG 2 (liver carcinoma), MCF 7 (breast carcinoma), HCT 116 (colon carcinoma), and Hep 2 (larynx carcinoma). On the basis of these results, the ES-EE as well as Compounds (1) and (3) seem to have potential as a novel cancer preventive agent.
Eruca Sativa LINN.: Pharmacognostical and Pharmacological Properties and Pharmaceutical Preparations
Asian Journal of Pharmaceutical and Clinical Research, 2019
Eruca sativa (jarjeer) is an annual herb (family Brassicaceae), which contains a wide range of chemicals and minerals with nutraceutical and organoleptic characteristics. Jarjeer was generally used as a food and traditionally mainly consumed due to its aphrodisiac properties. This crop known to contain various phytochemicals such as flavonoids, phenolic acids, terpens, carotenoids, tannins, glycosides, saponins, sterols, alkaloids, and other secondary metabolites. In leaves, kaempferol and its derivatives, glucosativin, are the main flavonoids and glucosinolate, respectively, while erucic acid and glucoerucin are the main fatty acid and glucosinolate, respectively. Medicinally, the plant has antibacterial, antidiabetic, antihypertensive, antiplatelet, and antioxidant activity and stimulates hair growth and other effects. Trails on topical pharmaceutical preparations involve the use of E. sativa which had been done. These preparations include creams and waxs which are intended to be ...
Antioxidant activity of Eria Alba extracts
The present study was performed to evaluate antioxidant activity of E. alba. The air dried powdered of plant material was extracted with petroleum ether, ethyl acetate and methanol and concentrated using rotary evaporator. The methanolic extract of E. alba and ethyl acetate fraction had significant scavenging effects with increasing concentration in the range of 100-400µg/ml. The methanolic extract showed significant antioxidant activity as compared to ethyl acetate and petroleum ether extract.
J. Pharm. Sci. & Res, 2017
The present study investigates the effect of the methanolic extract of E. sativa leaves on some oxidative stress markers as reactive oxygen species, total antioxidant capacity, metallothionein, cytochrome P450 (CYP1A1) and acetylcholinesterase concentrations and their correlations with hematological parameters (CBC profile) in male albino rats. Three experimental groups were used in this study which was 250 and 500 mg/kg body weight of 20 % methanolic of E. sativa leaves extract which given orally for 40 consecutive days and the third group treated with distilled water as a placebo-treated control group. The data of this study showed no significant differences in reactive oxygen species and cytochrome P450 concentrations between groups treated with E. sativa leaves extract and control group. Both dose 250 and 500 mg/ kg of E. sativa leaves extract could cause significant increasing in MT concentration while only 500 mg/ kg of E. sativa caused a significant increasing in acetylcholinesterase concentration compared with control group. In addition, there were non-significant differences in red blood cells count and indices while there were some significant differences in total and differential white blood cells count in treated male rats.
Iraqi journal of science, 2021
Eruca sativa, commonly known as rocket salad, is a popular vegetable to which a wide range of health benefits are attributed. This study aimed to examine the effects of the aqueous extract of E. sativa leaves on lipid profile and some minerals, such as calcium and magnesium, in blood of male albino mice under normal physiological condition. Two experiments were separately conducted, each with eighteen male albino mice divided into 3 equal groups, which included control, treated group (1), and treated group (2) which were orally administrated with 0, 0.2 and 0.4 mg/kg body weight, respectively, of the leaves extract for 28 days. In the first experiment, serum lipid profile, including cholesterol, triglyceride, HDL, LDL and VLDL, were estimated. In the second experiment, serum calcium and magnesium were estimated. The results showed that E. sativa have effects on lipid profile by decreasing cholesterol, triglyceride, and LDL levels, but increasing that of HDL. In addition, E. sativa i...
Anti-Inflammatory and Anti-Allergic Activity of Eruca Sativa Seed
International Journal of Current Pharmaceutical Research, 2015
The present study has been designed to examine the Anti-allergic and Anti-inflammatory activity of various seed extracts of Eruca sativa in-vivo model. Milk induced leukocytosis and Eosinophilia in mice, formaldehyde induced paw edema was carried out against n-hexane and ethanolic extracts in albino mice dose dependent manner. Results showed that n-hexane extract at 400 mg/kg p. o significantly inhibited (p < 0.01) the blood eosinophilia, leukocyte and differential leukocyte count. These results were also comparable to those of dexamethasone, the reference drug used in this study. In the model of edema induced by formalin, n-hexane fractions at 400 mg/kg body weight reduced significantly (p < 0.01) the formation of edema induced by formalin. The results suggested that the hexane of Eruca sativa seeds possessed potent anti-inflammatory and anti allergic properties. Keywords: Traditional medicines, Duncan’s Multiple Range Test, Green medicines, Eosinophils.
Molecules, 2022
Eruca sativa Mill. (E. sativa) leaves recently grabbed the attention of scientific communities around the world due to its potent bioactivity. Therefore, the present study investigates the metabolite profiling of the ethanolic crude extract of E. sativa leaves using high resolution-liquid chromatography-mass spectrometry (HR-LC/MS), including antibacterial, antioxidant and anticancer potential against human colorectal carcinoma cell lines. In addition, computer-aided analysis was performed for determining the pharmacokinetic properties and toxicity prediction of the identified compounds. Our results show that E. sativa contains several bioactive compounds, such as vitamins, fatty acids, alkaloids, flavonoids, terpenoids and phenols. Furthermore, the antibacterial assay of E. sativa extract showed inhibitory effects of the tested pathogenic bacterial strains. Moreover, the antioxidant activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) were found to be IC50 ...