Control of Ectoparasitic Mites in Honeybee (Apis mellifera L.) Colonies by Using Thymol and Oxalic Acid (original) (raw)

Thymol as control agent of mites (Varroa destructor) on honeybees (Apis mellifera)

Pakistan Journal of Agricultural Research, 2013

Studies were carried out on thymol as a controlling agent -1 for bee mites. In this study thymol powder (5mg hive ) was compared with formic acid 65 % and fluvalinate (strip) as standard control measure against Varroa mite. The mite mortality rate was recorded in honeybee colonies of Apis mellifera during 2011-12 at research farm of Beekeeping and Hill Fruit Pests Research Station, Rawalpindi. Analysis of variance for different treatments indicated (F=3.64, F>P=0.15) significantly different effect among treatments as well as for the control of mite. The average efficacy of thymol was 73.72% as compared to 69.21% by fluvalinate, 72.23% by formic acid and 13.1% in control. The reduction in the effectiveness of fluvalinate may be attributed to the development of resistance against fluvalinate strip.

Control of Ectoparasitic Mite Varroa Destructor in Honeybee (Apis Mellifera L.) Colonies by Using Different Concentrations of Oxalic Acid

Journal of Animal and Plant Sciences

The research work was carried out to determine the effects of oxalic acid (OA) on reducing ectoparasitic mite Varroa destructor Anderson and Trueman (Acari:Varroidae) populations in honeybee Apis mellifera linguistica (Hymenoptera: Apidae) colonies in the fall at Honey bee Research Institute, National Agricultural Research centre, Islamabad. Twenty honeybee colonies were used in this experiment. Colonies were divided into four groups of five colonies each. Oxalic acid was applied in sugar syrup with 4.2, 3. 2 and 2.1% concentrations. The OA with different concentrations was tricked directly on the adult honey bees in between two frames using a syringe applied thrice on different dates at five days interval. Average efficacy of OA with 3.2, 4.2 and 2.1 % was 95, 81 and 46 % respectively. No queens were lost, and there was no adult honeybee mortality in any of the colonies during the experiment. It can be concluded that 3.2% OA concentration are very effectively control varroa mite an...

Effect of Thymol and Formic Acid Against Ectoparasitic Brood Mite Tropilaelaps clareae in Apis mellifera Colonies

The study was conducted during March-April, 2007 with two groups each of four honeybee colonies at Honeybee Research Institute, NARC Islamabad. One group was treated with thymol and the other with formic acid. The 4 gm thymol (powdered form) and the formic acid 65% (20ml) were applied 4 times at weekly interval. It was found out that formic acid killed significantly higher number of mites as compared to thymol. The total honey production harvested from colonies treated with different acaricides remained the same.

Study of the Efficacy of Oxalic Acid and Thymovar (Thymol) against the Parasitic Mite of the Honey Bee, Varroa destrutor

Egyptian Academic Journal of Biological Sciences, 2020

Varroasis is a dangerous pathogen of the honey bee caused by the mite Varroa destructor, the aim of this work is to assess the effectiveness of two natural products (oxalic acid and thymol) in the fight against varroasis. The experiment covered 40 colonies in the central region of Algeria. Two doses of oxalic acid were used: 30 and 45 grams per liter of sugar syrup. For thymol (Thymovar), two treatments were carried out: ½ strip with one and two applications for each group of colonies. The 1/2 strip formulation in two applications at an interval of two weeks apart seems the most effective; the 45 gm solution of oxalic acid has a higher efficacy compared to that of 30 gm, that the effectiveness of oxalic acid is directly linked to the presence of brood at the time of the intervention. these two treatments are of control measure to be integrated by beekeepers as part of an alternative control strategy.

Assessing Repeated Oxalic Acid Vaporization in Honey Bee (Hymenoptera: Apidae) Colonies for Control of the Ectoparasitic Mite Varroa destructor

Journal of Insect Science, 2021

The American beekeeping industry continually experiences colony mortality with annual losses as high as 43%. A leading cause of this is the exotic, ectoparasitic mite, Varroa destructor Anderson & Trueman (Mesostigmata: Varroidae). Integrated Pest Management (IPM) options are used to keep mite populations from reaching lethal levels, however, due to resistance and/or the lack of suitable treatment options, novel controls for reducing mites are warranted. Oxalic acid for controlling V. destructor has become a popular treatment regimen among commercial and backyard beekeepers. Applying vaporized oxalic acid inside a honey bee hive is a legal application method in the U.S., and results in the death of exposed mites. However, if mites are in the reproductive stage and therefore under the protective wax capping, oxalic acid is ineffective. One popular method of applying oxalic is vaporizing multiple times over several weeks to try and circumvent the problem of mites hiding in brood cells...

Control of Tropilaelaps clareae Mite by Using Formic Acid and Thymol in Honey Bee Apis mellifera L. Colonies

Pakistan journal of zoology

The proposed research work was conducted in March, 2008 at Honeybee Research Institute of National Agricultural Research Centre, Islamabad on Apis mellifera lingustica honeybee colonies infested with the Tropilaelaps clareae. Treatments were given by dividing experimental 36 colonies into two groups (Formic acid and Thymol). Formic acid (70%) and Thymol were applied in six treatments including control (To=0.0 ml, T1=5ml, T2=10ml, T3=15ml, T4=20ml and T5= 25ml) and (To=0.0g, T1=5g, T2=10g, T3=15g, T4=20g, and T5=25g) respectively with three replications for five weeks. The treatments were given randomly by using complete randomized design (CRD). The maximum number 782 and 518 of mites collected in mite collection trays treated with 25 ml formic acid and 25 g of thymol. The highest efficacy of formic acid and thymol against T. clareae on honeybee was 97 and 95.3%. In formic acid group T5 (25 ml) yielded the maximum honey 22.54 kg/colony. In thymol group, T5 (25g) yielded the maximum h...

Efficacy of various botanicals and chemicals on ectoparasitic mite, Varroa destructor feeding on European honey bee, Apis mellifera

2017

The present investigation was carried out to evaluate the chemicals (Formic acid, Oxalic acid and Taufluvalinate) and botanicals (Artemesia annua, Matricaria chamomilla, Juglans regia and mixture A. annua + M. chamomilla + J. regia) under Complete Randomised Block Design (RCBD) for management of V. destructor during 2016 at Faculty of Agriculture, Wadura SKUAST-Kashmir. The results revealed that among chemicals, Taufluvalinate (2 strips per colony) was found highly effective against the V. destructor mite followed by Formic acid 85% @ 2 ml per colony but in case of botanicals green leaf extract mixture @ 150ml/colony was found highly effective against Varroa mite followed by M. chamomilla. The order of efficacy of treatments was Taufluvalinate (78.93%) > Formic acid (74.83%) > Oxalic acid (72.16 %) > GLE mixture (67.10 %) > M. chamomilla (63.83%) > A. annua (61.87%) > J. regia (58.34 %). Bio leaf extracts need to be integrated in such a way that there may be no haz...

Control of Varroa destructor Mite by Using Oxalic Acid, Formic Acid in Honey Bee Apis mellifera L. Colonies in Pakistan

2013

2 Abstract: The proposed research work was conducted in January, 2012 at Honeybee Research Institute of National Agricultural Research Centre, Islamabad on Apis mellifera lingustica honeybee colonies infested with the Varroa destructor. Treatments were given by dividing experimental colonies into three groups (formic acid, oxalic acid and control). Oxalic acid 3.2% (T ), Formic acid 65% (T ) and control (T ) were applied in seven 1 2 3 replications each for four weeks. The treatments were given randomly by using complete randomized design (CRD). The maximum mean number 635 ± 4.31 (Mean ± SE), 305±3.82 (Mean ± SE) and 103±3.30 (Mean ± SE) of mites collected in mite collection trays treated with T , T and T respectively and the difference between the 12 3 treatments was highly significant. The efficacy and honey yield from the experimental colonies were also highly significant difference than the controls. The highest mean efficacy 91± 0.26 (Mean ± SE) and honey yield 24± 0.44 (Mean ±...

Use of oxalic acid to control Varroa destructor in honeybee (Apis mellifera L.) colonies

Turkish Journal of Veterinary and Animal Sciences, 2009

This study was carried out to determine the effects of oxalic acid (OA) on reducing Varroa mite (Varroa destructor) populations in honeybee (Apis mellifera L.) colonies in the fall. Twenty honeybee colonies, in wooden Langstroth hives, were used in this experiment. Average Varroa infestation levels (%) of the OA and control groups were 25.87% and 24.57% on adult workers before the treatments. The OA treatments were applied twice, on 3 November and 13 November 2006. Average Varroa infestation levels were 5.24% and 31.43% after the first application and 2.87% and 41.74% after the second application in the OA and control groups, respectively. Average efficacy of OA was 93.40%. No queens were lost, and there was no brood or adult honeybee mortality in any of the colonies during the experiment.