Effects of Temperature, pH and Lactic Acid Concentration on the Phase Behaviour of Aqueous Solutions of Poly(ethylene glycol) and Poly(ethyleneimine) (original) (raw)

The present work is part of a broader study devoted to the application of aqueous two-phase systems (ATPSs) for in situ separation of low molar mass organic acids in the biotechnology industry. The ATPSs studied comprise poly(ethyleneglycol) (PEG) and poly(ethyleneimine) PEI. The PEG average molar mass was 4000 gmol -1 with a polydispersity index of 1.05, and the PEI molar mass was 25000 gmol -1 with a polydispersity index of about 2.5. Measurements of the binodal curves of the ATPSs were performed at T = (25 and 36) 0 C, p = 1 bar, 5 ≤ pH ≤ 9 and at two different concentrations of lactic acid. The partition coefficient of the lactic acid was also measured at T = 36 0 C and pH = 7.5.

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