The Physical and Genetic Map Surrounding theLystGene on Mouse Chromosome 13 (original) (raw)
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Immunogenetics, 1987
Previous work has demonstrated linkage between Ly-6, 11-30, and a locus, Ril-1, that affects susceptibility to radiation-induced leukemia. Results of preliminary linkage analyses suggested further that the cluster might be linked to Ly-11 on the proximal portion of mouse chromosome 2. Using molecular probes to examine somatic cell lines and recombinant inbred and congenic strains of mice, we have re-evaluated these linkage relationships. A cloned genomic DNA fragment derived from a retroviral site has been used to define a novel locus, Pol-5, that is tightly linked to both H-30 and Ril-1 as shown by analysis of the B6.C-H-30 ~ congenic mouse strain. Following the segregation of the Pol-5 mouse-specific DNA fragment in a series of somatic cell hybrids carrying various combinations of mouse chromosomes on a rat or Chinese hamster background mapped Pol-5 to mouse chromosome 15. During the course of these studies, re-A, agouti; Abl, cellular homolog of the Abelson leukemia virus oncogene; Ada, adenosine deaminase; Ak-1, adenylate kinase-1; AXB, A/J x C57BL/6J recombinant inbred strain; B2m, beta-2 microglobulin; BXA, C57BL/6J X A/J recombinant inbred strain; BXD, C57BL/6J x DBA/2J recombinant inbred strain; BXH, C57BL/6J×C3H/HeJ recombinant inbred strain; CXB, BALB/cBy × C57BL/6By recombinant inbred strain; DNA, deoxyribonucleic acid; Eh, hairy ears; Fpgs, folypolyglutamyl synthetase; FXI, fractionated x-irradiation; Gdc-l, glycerol phosphate debydrogenase-1; l12r,/I.-2 receptor; Ins-3, a novel insulinlike gene; Int-1, mammary tumor integration site-1; Itp, inosine triphosphatase; Krt-1, the locus designated here includes a cluster of at least three keratin genes; LTR, long terminal repeat; Ly, lymphocyte; Lv-6, lymphocyte antigen-6; Ly-11, lymphocyte antigen-11; MIH, minor histocompatibility;
Fine Haplotype Structure of a Chromosome 17 Region in the Laboratory and Wild Mouse
Genetics, 2008
Extensive linkage disequilibrium among classical laboratory strains represents an obstacle in the high-resolution haplotype mapping of mouse quantitative trait loci (QTL). To determine the potential of wild-derived mouse strains for fine QTL mapping, we constructed a haplotype map of a 250-kb region of the t-complex on chromosome 17 containing the Hybrid sterility 1 (Hst1) gene. We resequenced 33 loci from up to 80 chromosomes of five mouse (sub)species. Trans-species single-nucleotide polymorphisms (SNPs) were rare between Mus m. musculus (Mmmu) and Mus m. domesticus (Mmd). The haplotypes in Mmmu and Mmd differed and therefore strains from these subspecies should not be combined for haplotype-associated mapping. The haplotypes of t-chromosomes differed from all non-t Mmmu and Mmd haplotypes. Half of the SNPs and SN indels but only one of seven longer rearrangements found in classical laboratory strains were useful for haplotype mapping in the wild-derived M. m. domesticus. The largest Mmd haplotype block contained three genes of a highly conserved synteny. The lengths of the haplotype blocks deduced from 36 domesticus chromosomes were in tens of kilobases, suggesting that the wild-derived Mmd strains are suitable for fine interval-specific mapping.
American journal of human genetics, 1996
Chediak-Higashi syndrome (CHS) is an autosomal recessive disorder characterized by hypopigmentation or oculocutaneous albinism and severe immunologic deficiency with neutropenia and lack of natural killer (NK) cell function. Most patients die in childhood from pyogenic infections or an unusual lymphoma-like condition. A hallmark of the disorder is giant inclusion bodies seen in all granule-containing cells, including granulocytes, lymphocytes, melanocytes, mast cells, and neurons. Similar ultrastructural abnormalities occur in the beige mouse, which thus has been suggested to be homologous to human CHS. High-resolution genetic mapping has indicated that the bg gene region of mouse chromosome 13 is likely homologous to the distal portion of human chromosome 1q. Accordingly, we carried out homozygosity mapping using markers derived from distal human chromosome 1q in four inbred families or probands with CHS. Our results indicate that the human CHS gene maps to an 18.8-cM interval in c...
New polymorphic markers in the vicinity of the pearl locus on mouse Chromosome 13
Mammalian Genome, 1996
We have used a Mus domesticus/-Mus spretus congenic animal that was selected for retention of Mus spretus DNA around the pearl locus to create a highly polymorphic region suitable for screening new markers. Representation difference analysis (RDA) was performed with either DNA from the congenic animal or C57BL/6J as the driver for subtraction. Four clones were identified, characterized, and converted to PCR-based polymorphic markers. Three of the four markers equally subdivide a 10-cM interval containing the pearl locus, with the fourth located centromeric to it. These markers have been placed on the mouse genetic map by use of an interspecific backcross panel between Mus domesticus (C57BL/6J) and Mus spretus generated by The Jackson Laboratory.
Molecular biology and …, 1987
The presence of the Ll sequences, L 1 Md4 next to the pseudogene ph3 and 112 found in the twelfth intron of the albumin gene, in certain strains of laboratory mice but not of others has led to the suggestion that these sequences were recent insertions into the Mus mus domesticus genome. To be sure that they are really recent insertions and not relics of an ancestral chromosome, we investigated the presence or absence of these sequences in populations of wild mice belonging to the semispecies M. m. domesticus and M. m. muscuIus as well as in other species of the genus A4us and in related murids. The sequence 112 in the albumin gene was found in 34% of the chromosomes of the wild mice belonging to M. m. domesticus and to a lesser extent (6%) in A4. m. musculus. Of 114 M. m. domesticus chromosomes, L 1 Md4 was found in only nine, seven of which came from the same locality. Its presence was associated with the haplotype HbbP, which is relatively rare in European populations of M. musculus. Since there was no evidence for the presence of these two Ll sequences in more distantly related species, we conclude that they are recent insertions in the M. musculus genome.
Mammalian Genome, 1992
Genome Mapping in Lunteren, The Netherlands, held October 1991. This report is intended to complement the previous one (Blank et al. 1991) and has an abbreviated text. It is structured so as to emphasize new mapping data that have become available since the 1990 meeting. The format of the report and the accompanying genetic map have not been changed.
Genetics
Inheritance of restriction fragment length polymorphisms associated with four anonymous DNA markers (DI2Nyu1, 2, 3 and 4 ) , the Fos proto-oncogene, the Mh-9 viral integration site, and the a,antitrypsin (Aat-I) and immunoglobulin heavy chain (Igh) gene families in the mouse has been followed in a backcross experiment. A Bayesian multilocus map-building strategy yielded the map: centromere-D12Nyu2-10 cM-DI2Nyul-2 cM-D12Nyu3-15 cM-Fos-1 cM-D12Nyu4-2 cM-Mh-9-8 cM-Aat-1-17 cM-Igh-C. A map constructed from male meiotic data was substantially shorter than one constructed from female meiotic data. Significant interference was observed for the linkage group. T w o groups of markers studied in recombinant inbred strains of mice could be interpolated into the map: Es-25, D12Nyul0, D12Nyu7 and Apob form a cluster proximal to D12Nyu2, and Ly-18, Ah, and D12Nyu5 form a cluster between D12Nyu2 and Dl2Nyul. These data establish an unambiguously ordered linkage group including Zgh and Aat-1 that spans most of chromosome 12.